From 2010.igem.org
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| - | [[Team:Caltech/Notebook|Back to Notebook]]
| + | {{:Team:Caltech/WeekTemplate}} |
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| | ==Monday 6/14== | | ==Monday 6/14== |
Latest revision as of 22:56, 16 July 2010
iGEM 2010
Home
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Project Details
Protocols
Completed Systems
Notebook
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Human Impact
References
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Monday 6/14
- Discussed procuring items & lab space.
- Prepared for SURF start.
Tuesday 6/15
- Created GANTT Chart (right) detailing our work plan for the summer.
 GANTT Chart diagramming our planned progress throughout the summer. - Detailed possible side projects, open to any member as time permits:
- Agarase
- pH/Environmental Response
- Phototaxis
- Colored plastic
- Activa (transglutaminase)
- Procured supplies from stock room
- Cleaned out lab space
- Prepared LB-amp media & plates
Wednesday 6/16
- Procured remaining antibiotics & sample of XL1 Blue cells
- Prepare orders for Sigma, NEB, and others
- Sigma: Crosslinking compounds, substrates (linseed oil & soybean oil)
- NEB: Biobrick assembly kit, Ladders, polymerase
- Prepared list of biobricks to order
- Addl Lysis bricks
- Agarase bricks
Thursday 6/17
- Made stock solutions for transformation and subsequent culturing
- Transformed XL-1 Blue E. Coli with selected BioBricks
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_J09250 J09250], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124017 K124017], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124014 K124014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13601 I13601], pBAD 18 plasmid
- Ordered more BioBricks:
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112012 K112012]
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K108001 K108001]
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112806 K112806]
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284001 K284001]
- [http://partsregistry.org/wiki/index.php?title=Part:BBa_I737020 I737020]
(We also wanted to order sequencing primers [http://partsregistry.org/wiki/index.php?title=Part:BBa_G00100 G00100] & [http://partsregistry.org/wiki/index.php?title=Part:BBa_G00101 G00101], but apparently those aren't sent to teams.)
Friday 6/18
- Checked transformation
- Minipreped transformed bacteria
- Checked the plasmid concentration in the mini-prep solution
- Made glycerol stocks from the transformed plasmid
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