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Team:Caltech/Week 1
From 2010.igem.org
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| - | + | {{Caltech_iGEM_10| | |
| + | Content= | ||
| + | {{:Team:Caltech/WeekTemplate}} | ||
| - | ==Monday== | + | ==Monday 6/14== |
* Discussed procuring items & lab space. | * Discussed procuring items & lab space. | ||
* Prepared for SURF start. | * Prepared for SURF start. | ||
| - | ==Tuesday== | + | ==Tuesday 6/15== |
| - | * Created GANTT Chart ( | + | * Created GANTT Chart (right) detailing our work plan for the summer. |
[[Image:IGEM_Gantt_Chart.gif|thumb|GANTT Chart diagramming our planned progress throughout the summer.]] | [[Image:IGEM_Gantt_Chart.gif|thumb|GANTT Chart diagramming our planned progress throughout the summer.]] | ||
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* Cleaned out lab space | * Cleaned out lab space | ||
* Prepared LB-amp media & plates | * Prepared LB-amp media & plates | ||
| - | ==Wednesday== | + | ==Wednesday 6/16== |
* Procured remaining antibiotics & sample of XL1 Blue cells | * Procured remaining antibiotics & sample of XL1 Blue cells | ||
* Prepare orders for Sigma, NEB, and others | * Prepare orders for Sigma, NEB, and others | ||
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** Addl Lysis bricks | ** Addl Lysis bricks | ||
** Agarase bricks | ** Agarase bricks | ||
| + | ==Thursday 6/17== | ||
| + | *Made stock solutions for transformation and subsequent culturing | ||
| + | *Transformed XL-1 Blue E. Coli with selected BioBricks | ||
| + | **[http://partsregistry.org/wiki/index.php?title=Part:BBa_J09250 J09250], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124017 K124017], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K124014 K124014], [http://partsregistry.org/wiki/index.php?title=Part:BBa_R0082 R0082], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0015 B0015], [http://partsregistry.org/wiki/index.php?title=Part:BBa_B0034 B0034], [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13601 I13601], pBAD 18 plasmid | ||
| + | * Ordered more BioBricks: | ||
| + | ** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112012 K112012] | ||
| + | ** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K108001 K108001] | ||
| + | ** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K112806 K112806] | ||
| + | ** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K284001 K284001] | ||
| + | ** [http://partsregistry.org/wiki/index.php?title=Part:BBa_I737020 I737020] | ||
| + | (We also wanted to order sequencing primers [http://partsregistry.org/wiki/index.php?title=Part:BBa_G00100 G00100] & [http://partsregistry.org/wiki/index.php?title=Part:BBa_G00101 G00101], but apparently those aren't sent to teams.) | ||
| + | ==Friday 6/18== | ||
| + | *Checked transformation | ||
| + | *Minipreped transformed bacteria | ||
| + | **Checked the plasmid concentration in the mini-prep solution | ||
| + | *Made glycerol stocks from the transformed plasmid | ||
| + | }} | ||
Latest revision as of 22:56, 16 July 2010
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People
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 Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15
Monday 6/14
Tuesday 6/15
Wednesday 6/16
Thursday 6/17
(We also wanted to order sequencing primers [http://partsregistry.org/wiki/index.php?title=Part:BBa_G00100 G00100] & [http://partsregistry.org/wiki/index.php?title=Part:BBa_G00101 G00101], but apparently those aren't sent to teams.) Friday 6/18
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