Team:KIT-Kyoto/Notebook/Promoters

From 2010.igem.org

(Difference between revisions)

Latest revision as of 21:48, 27 October 2010

Home/Home > Notebook > Promoters

Promoters

>>return to Sept.29^thu

K121013

n=1

10min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.373	0.310	0.290	0.293	0.328	0.272	0.317	0.338
	0.372	0.3130	0.274	0.285	0.325	0.261	0.300	0.327

20min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.346	0.329	0.321	0.272	0.366	0.281	0.479	0.324
	0.353	0.343	0.320	0.275	0.356	0.280	0.461	0.327

30min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.327	0.289	0.331	0.356	0.352	0.353	0.344	0.474
	0.339	0.293	0.333	0.365	0.358	0.368	0.340	0.467

40min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.439	0.463	0.416	0.466	0.461	0.319	0.475	0.419
	0.450	0.468	0.401	0.488	0.441	0.314	0.459	0.418

50min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.314	0.445	0.355	0.452	0.342	0.353	0.442	0.392
	0.310	0.491	0.370	0.471	0.338	0.348	0.424	0.392

60min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.468	0.478	0.459	0.459	0.446	0.354	0.515	0.420

70min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.452	0.4480	0.453	0.510	0.414	0.456	0.416	0.435
	0.434	0.434	0.521	0.400	0.421	0.428	0.402	0.423

80min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.439	0.458	0.450	0.417	0.510	0.248	0.401	0.428
	0.427	0.430	0.426	0.413	0.477	0.248	0.401	0.432

K121013	H₂O₂
	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
10min	0.373	0.311	0.281	0.289	0.326	0.266	0.307	0.333
20min	0.350	0.336	0.320	0.274	0.361	0.280	0.470	0.325
30min	0.333	0.291	0.332	0.361	0.355	0.361	0.342	0.471
40min	0.444	0.465	0.408	0.477	0.451	0.316	0.467	0.419
50min	0.312	0.468	0.363	0.462	0.340	0.351	0.433	0.392
60min	0.468	0.478	0.459	0.459	0.446	0.354	0.515	0.420
70min	0.443	0.441	0.487	0.455	0.417	0.442	0.409	0.429
80min	0.433	0.444	0.436	0.415	0.493	0.248	0.401	0.430

n=2

10min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.324	0.282	0.327	0.300	0.329	0.301	0.306	0.362
	0.324	0.286	0.315	0.299	0.320	0.306	0.306	0.367
	0.321	0.294	0.313	0.300	0.327	0.310	0.315	0.367

20min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.464	0.458	0.436	0.405	1.24	0.440	0.380	0.439
	0.438	0.432	0.400	0.376	1.22	0.425	0.350	0.452
	0.419	0.421	0.399	0.373	1.23	0.413	0.374	0.456

30min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.346	0.353	0.441	0.333	0.426	0.385	0.364	0.402
	0.353	0.345	0.432	0.340	0.409	0.372	0.368	0.416
	0.346	0.346	0.416	0.342	0.401	0.366	0.360	0.405

40min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.399	0.461	0.413	0.541	0.416	0.395	0.510	0.372
	0.370	0.461	0.396	0.524	0.403	0.377	0.506	0.377
	0.368	0.450	0.397	0.54	0.396	0.377	0.480	0.379

50min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.500	0.429	0.355	0.441	0.566	0.462	0.450	0.414
	0.469	0.407	0.341	0.405	0.564	0.421	0.418	0.408
	0.434	0.390	0.340	0.384	0.575	0.406	0.409	0.413

60min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.405	0.398	0.395	0.365	0.442	0.414	0.403	0.411
	0.388	0.405	0.386	0.365	0.415	0.406	0.413	0.409
	0.403	0.404	0.389	0.370	0.405	0.414	0.417	0.405

70min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.426	0.600	0.557	0.413	0.454	0.450	0.434	0.387
	0.426	0.545	0.504	0.421	0.454	0.462	0.429	0.393
	0.436	0.506	0.500	0.431	0.465	0.466	0.446	0.404

80min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.457	0.445	0.456	0.457	0.464	0.466	0.450	0.417
	0.474	0.458	0.468	0.471	0.477	0.484	0.465	0.430
	0.474	0.460	0.470	0.485	0.481	0.490	0.468	0.416

90min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.469	0.458	0.491	0.475	0.483	0.444	0.533	0.446
	0.482	0.464	0.450	0.481	0.493	0.443	0.543	0.450
	0.491	0.467	0.510	0.486	0.506	0.45	0.540	0.448

K121013	H₂O₂
	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
10min	0.323	0.287	0.318	0.299	0.326	0.306	0.309	0.365
20min	0.440	0.437	0.412	0.385	1.23	0.426	0.368	0.449
30min	0.348	0.348	0.429	0.338	0.412	0.374	0.364	0.408
40min	0.468	0.409	0.345	0.410	0.568	0.430	0.425	0.412
50min	0.399	0.402	0.390	0.367	0.420	0.411	0.411	0.408
60min	0.429	0.550	0.520	0.422	0.457	0.459	0.436	0.395
70min	0.469	0.455	0.465	0.471	0.474	0.480	0.461	0.421
80min	0.481	0.463	0.483	0.481	0.494	0.446	0.539	0.448

n=3

10min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.674	0.662	0.684	0.663	0.723	0.646	0.667	0.682
	0.675	0.667	0.679	0.667	0.723	0.655	0.659	0.698
	0.679	0.662	0.682	0.661	0.731	0.661	0.647	0.693

20min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.715	0.704	0.712	0.705	0.695	0.696	0.768	1.06
	0.715	0.690	0.698	0.706	0.694	0.695	0.753	1.04
	0.714	0.708	0.705	0.699	0.709	0.701	0.736	1.04

30min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.809	0.770	0.664	0.704	0.759	0.686	0.844	0.726
	0.792	0.760	0.659	0.715	0.759	0.692	0.806	0.717
	0.811	0.775	0.681	0.711	0.758	0.699	0.816	0.716

40min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.768	0.729	0.713	0.704	0.800	0.790	0.776	0.742
	0.766	0.726	0.703	0.710	0.796	0.788	0.769	0.750
	0.772	0.731	0.707	0.717	0.802	0.785	0.773	0.759

50min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.722	0.729	0.755	0.784	0.814	0.842	0.838	0.918
	0.733	0.732	0.759	0.786	0.820	0.848	0.843	0.930
	0.740	0.735	0.767	0.793	0.825	0.846	0.838	0.936

60min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.901	0.885	0.981	0.866	0.834	0.888	0.844	0.968
	0.908	0.897	0.988	0.873	0.819	0.895	0.835	0.963
	0.914	0.898	0.985	0.887	0.843	0.881	0.833	0.956

70min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.942	0.985	0.967	0.917	0.914	0.910	0.970	0.932
	0.941	0.972	0.955	0.900	0.911	0.897	0.940	0.931
	0.942	0.966	0.957	0.900	0.903	0.879	0.953	0.941

80min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	1.04	1.01	0.994	1.02	1.03	1.01	0.995	1.17
	1.02	1.01	0.987	1.01	0.969	0.995	0.974	1.14
	1.03	1.02	1.01	1.00	0.976	0.999	0.979	1.14

90min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	0.979	1.01	1.05	1.02	1.04	0.989	0.933	1.12
	0.997	1.01	1.06	1.01	0.995	1.01	0.951	1.11
	0.982	1.01	1.07	1.04	1.02	1.00	0.949	1.10

K121013	H₂O₂
	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
10min	0.676	0.663	0.682	0.664	0.726	0.654	0.658	0.691
20min	0.715	0.701	0.705	0.703	0.700	0.698	0.752	1.05
30min	0.804	0.768	0.668	0.710	0.759	0.693	0.822	0.720
40min	0.769	0.725	0.707	0.710	0.799	0.788	0.772	0.750
50min	0.732	0.732	0.760	0.787	0.820	0.845	0.840	0.928
60min	0.942	0.974	0.960	0.905	0.909	0.895	0.955	0.935
70min	1.03	1.02	0.997	1.01	0.990	1.00	0.983	1.15
80min	0.986	1.01	1.06	1.02	1.02	0.999	0.944	1.11
90min	0.986	1.01	1.06	1.03	1.02	0.999	0.944	1.11

ahpC

n=1

10min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	6.36	6.55	6.64	5.95	7.19	9.85	10.4	12.0
	6.48	6.71	6.73	6.11	7.34	10.2	11.0	12.4
	6.52	6.72	6.78	6.15	7.46	10.4	11.3	12.8

20min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	7.82	6.91	7.31	6.92	7.26	11.4	12.6	13.6
	7.89	7.04	7.47	6.97	7.37	11.4	12.9	13.9

30min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	8.17	8.20	8.62	8.71	8.97	11.3	13.6	16.1
	8.32	8.30	8.79	8.83	8.98	11.4	13.9	16.1

40min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	8.37	8.75	9.20	8.52	8.86	11.5	14.9	16.0
	8.39	8.73	9.19	8.55	9.05	11.9	15.3	16.2

50min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	10.3	10.5	11.5	11.1	11.9	11.1	16.6	19.8
	10.5	10.8	11.8	11.5	12.3	11.7	17.2	20.2

60min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	11.9	13.3	13.0	13.3	13.8	16.0	19.4	22.5
	12.1	13.7	13.3	13.5	14.0	16.3	19.8	23.1

70min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	16.7	16.0	15.8	15.7	18.0	21.2	24.2	25.0
	17.2	16.6	16.4	16.0	18.5	21.5	24.9	25.2

80min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	21.2	27.0	22.2	23.4	23.5	25.6	28.9	31.3
	22	27.7	22.8	24.1	24.1	25.7	29.1	31.6

ahpC	H₂O₂
	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
10min	6.45	6.66	6.72	6.07	7.33	10.2	10.9	12.4
20min	7.86	6.98	7.39	6.94	7.32	11.4	12.8	13.7
30min	8.25	8.25	8.71	8.77	8.98	11.3	13.8	16.1
40min	8.38	8.74	9.20	8.53	8.95	11.7	15.1	16.1
50min	10.4	10.6	11.7	11.3	12.1	11.4	16.9	20.0
60min	12.0	13.5	13.2	13.4	13.9	16.1	19.6	22.8
70min	16.9	16.3	16.1	15.9	18.3	21.3	24.6	25.1
80min	16.9	16.3	16.1	15.9	18.3	21.3	24.6	25.1

n=2

10min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	7.33	7.58	7.41	7.47	8.27	8.71	10.8	10.7
	7.32	7.53	7.30	7.35	8.26	8.49	11.3	11.6
	7.34	7.52	7.28	7.29	8.26	8.48	11.5	11.8

20min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	8.05	8.17	8.03	8.19	9.05	10.7	11.4	13.2
	8.19	8.27	8.19	8.54	9.21	10.9	11.6	13.5
	8.34	8.35	8.33	8.57	9.31	11.1	11.8	13.8

30min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	8.70	8.65	8.79	8.76	10.7	11.1	12.4	14.5
	8.89	8.76	8.97	8.84	10.7	10.9	12.5	14.6
	8.96	8.94	9.10	8.91	10.8	11.1	12.9	15.0

40min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	9.36	9.20	8.83	9.08	10.5	12.7	13.2	15.8
	9.60	9.38	8.96	9.20	10.6	12.8	3.44	15.9
	9.69	9.40	9.12	9.32	10.8	12.8	13.6	15.9

50min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	10.1	10.5	10.3	10.2	11.3	12.8	14.6	17.9
	10.5	10.9	10.6	10.7	11.5	13.1	14.8	18.2
	10.7	11.1	10.8	10.8	11.8	13.4	15.1	18.4

60min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	11.2	12.0	11.9	12.5	12.8	14.4	16.5	19.2
	11.3	12.2	12.1	12.7	13.1	14.5	16.6	19.2
	11.5	12.5	12.4	13.1	13.3	14.9	16.9	19.3

70min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	13.2	12.3	13.6	13.4	15.8	17.6	18.4	21.3
	12.8	12.3	13.3	13.5	15.4	17.4	18.0	20.5
	12.9	13.1	14.2	13.7	16.1	17.9	18.9	20.9

80min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	16.2	18.1	17.9	18.3	18.3	20.9	17.5	22.9
	16.1	18.2	17.9	18.5	18.1	20.5	17.3	22.3
	16.4	18.9	18.4	19.3	18.7	21.1	18.2	23.4

11.6

ahpC	H₂O₂
	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
10min	7.33	7.54	7.33	7.37	8.26	8.56	11.2	11.4
20min	8.20	8.26	8.18	8.43	9.19	10.9	13.5
30min	8.85	8.78	8.95	8.84	10.7	11.0	12.6	14.7
40min	9.55	9.33	8.97	9.20	10.6	12.8	13.4	15.8
50min	10.4	10.8	10.6	10.6	11.5	13.1	14.8	18.2
60min	11.3	12.2	12.2	12.8	13.1	14.6	16.7	19.2
70min	13.0	12.5	13.7	13.5	15.8	17.6	18.4	20.9
80min	16.3	18.4	18.1	18.7	18.4	20.8	17.7	22.9

n=3

10min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	5.52	5.37	5.72	5.53	6.00	6.93	7.63	7.77
	5.63	5.46	5.69	5.49	5.95	6.93	7.67	7.71
	5.56	5.42	5.69	5.44	5.95	6.97	7.80	7.80

20min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	6.11	5.92	5.70	5.54	5.77	7.36	8.28	10.0
	6.05	5.89	5.50	5.43	5.57	7.17	7.96	9.71
	6.08	5.93	5.55	5.51	5.67	7.28	8.22	9.98

30min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	6.02	5.81	6.29	6.03	6.30	7.78	8.56	10.1
	6.03	5.81	6.26	6.03	6.29	7.42	8.52	9.96
	6.12	5.87	6.31	6.10	6.35	7.56	8.63	9.95

40min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	6.22	5.90	6.46	6.01	5.74	12.2	8.66	10.3
	6.30	6.07	6.61	6.20	5.83	12.7	8.89	10.4
	6.39	6.15	6.66	6.32	6.00	12.9	9.10	10.5

50min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	6.59	6.48	6.77	6.74	6.92	8.14	9.59	12.1
	6.72	6.66	6.95	6.90	7.00	8.33	9.74	12.1
	6.86	6.80	7.10	7.05	7.11	8.47	10.0	12.3

60min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	6.36	7.38	7.05	7.15	7.84	8.82	10.4	11.7
	6.43	7.60	7.25	7.38	7.94	8.85	10.5	11.8
	6.71	7.79	7.42	7.56	8.10	9.02	10.6	11.9

70min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	9.19	9.16	9.25	9.09	8.83	11.7	12.5	13.0
	9.40	9.31	9.21	9.15	8.71	11.5	12.4	13.3
	9.61	9.56	9.37	9.36	8.71	11.8	12.6	13.4

80min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	11.0	11.1	11.5	10.9	12.0	13.7	15.4	15.4
	11.2	11.3	11.6	11.1	12.2	13.5	15.2	15.3
	11.3	11.7	11.8	11.6	12.4	13.9	15.8	15.4

90min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	16.0	17.6	15.0	14.6	15.3	16.4	17.1	17.3
	16.2	17.8	15.1	14.7	15.5	16.4	17.2	17.5
	16.4	18.1	15.3	15.0	15.7	16.6	17.4	17.7

sufA

n=1

10min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	1.24	1.13	1.33	1.26	1.29	1.49	2.62	4.24
	1.278	1.16	1.34	1.29	1.27	1.52	2.74	4.28
	1.29	1.16	1.33	1.29	1.27	1.55	2.75	4.36

20min	1.34	1.27	1.39	1.32	1.40	1.53	3.07	5.20
	1.34	1.25	1.38	1.32	1.43	1.56	3.08	5.09

30min	1.31	1.25	1.39	1.30	1.34	1.65	2.91	5.03
30min	1.33	1.29	1.41	1.32	1.37	1.67	2.94	4.97

40min	1.40	1.41	1.39	1.36	1.42	1.62	3.07	5.31
40min	1.38	1.38	1.37	1.34	1.39	1.64	3.17	5.27

50min	1.72	0.983	1.23	1.38	1.40	1.53	3.17	5.49
50min	1.23	0.994	1.26	1.35	1.39	1.59	3.17	5.58

60min	1.37	1.50	1.62	1.23	1.58	1.90	3.40	5.85
60min	1.43	1.58	1.69	1.27	1.61	1.92	3.49	5.92

70min	1.77	1.77	2.46	1.82	2.11	2.20	4.00	6.13
70min	1.90	1.87	2.58	1.92	2.18	2.24	4.00	6.10

80min	2.81	2.76	2.86	2.84	1.80	3.20	5.44	3.32
80min	3.07	3.01	2.17	3.01	1.91	3.29	5.52	3.26

sufA	H₂O₂
	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
10min	1.27	1.15	1.33	1.28	1.28	1.52	2.70	4.29
20min	1.34	1.26	1.38	1.32	1.42	1.54	3.07	5.15
30min	1.32	1.27	1.40	1.31	1.35	1.66	2.92	5.00
40min	1.39	1.39	1.38	1.35	1.40	1.63	3.12	5.29
50min	1.47	0.989	1.24	1.36	1.40	1.56	3.17	5.53
60min	1.40	1.54	1.65	1.25	1.60	1.91	3.45	5.89
70min	1.84	1.82	2.52	1.87	2.14	2.22	3.99	6.11
80min	2.94	2.89	2.51	2.93	1.86	3.24	5.48	3.29

n=2

10min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	3.03	3.12	3.16	2.96	3.15	3.12	3.49
	3.03	3.05	3.10	2.93	3.10	3.08	3.54	4.07
	3.01	3.07	3.13	2.95	3.11	3.06	3.46	4.07

20min	3.11	3.10	2.94	2.99	3.48	2.95	3.67	4.41
	3.07	3.06	2.91	2.57	3.46	3.00	3.65	4.52
	3.03	3.10	2.94	3.05	3.46	2.98	3.89	4.43

30min	3.30	1.93	3.28	3.00	3.19	3.18	3.80	4.57
	3.21	1.81	3.16	2.98	3.15	3.15	3.74	4.59
	3.17	1.90	3.16	3.03	3.22	3.17	3.80	4.67

40min	3.09	3.15	2.97	2.82	3.14	3.12	3.48	4.53
	3.10	3.26	3.00	2.88	3.15	3.14	3.60	4.51
	3.07	3.26	3.01	2.90	3.14	3.17	3.63	4.54

50min	3.15	3.06	3.06	3.10	3.14	3.37	3.78	4.84
	3.11	3.11	3.13	3.18	3.25	3.39	3.86	4.84
	3.11	3.10	3.09	3.14	3.22	3.37	3.82	4.83

60min	3.07	3.11	3.07	3.38	3.21	3.31	3.69	4.84
	3.00	3.10	3.05	3.41	3.27	3.39	3.75	4.93
	3.01	3.14	3.07	3.45	3.26	3.38	3.73	5.01

70min	3.64	3.29	3.37	3.52	3.33	3.41	4.08	4.99
	3.51	3.11	3.21	3.36	3.14	3.26	3.37	4.75
	3.59	3.12	3.27	3.40	3.19	3.26	3.78	4.69

80min
	3.79	3.28	4.29	3.07	3.27	3.39	4.04	4.81
	3.77	3.29	4.28	3.03	3.22	3.39	4.01	4.71
	3.76	3.30	4.27	3.04	3.26	3.45	3.99	4.82

sufA	H₂O₂
	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
10min	3.02	3.08	3.13	2.95	3.12	3.09	3.50	4.11
20min	3.07	3.09	2.93	2.87	3.47	2.98	3.73	4.45
30min	3.23	1.88	3.21	3.01	3.18	3.17	3.78	4.61
40min	3.09	3.22	2.99	2.87	3.15	3.14	3.57	4.53
50min	3.12	3.09	3.09	3.14	3.20	3.38	3.82	4.84
60min	3.02	3.12	3.07	3.41	3.24	3.36	3.72	4.93
70min	3.58	3.17	3.28	3.43	3.22	3.31	3.74	4.81
80min	3.77	3.29	4.28	3.05	3.25	3.41	4.01	4.78

n=3

10min	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
	2.10	1.87	2.03	2.09	2.08	2.15	2.82	3.49
	2.07	1.87	1.99	2.07	2.00	2.12	2.76	3.51
	2.02	1.85	1.98	2.05	1.98	2.12	2.81	3.59

20min
	2.29	2.62	2.24	2.07	2.03	2.15	2.83	4.25
	2.24	2.00	2.20	2.08	2.01	2.14	2.86	4.19
	2.25	2.02	2.19	2.09	2.02	2.16	2.88	4.29

30min
	2.04	1.95	1.89	1.96	2.09	2.07	2.90	4.09
	2.00	1.86	1.87	1.96	2.06	2.09	2.96	4.09
	2.01	1.91	1.91	1.98	2.08	2.11	2.99	4.10

40min	1.95	1.93	2.16	2.04	2.06	2.10	2.70	4.33
	1.94	1.87	2.17	2.06	2.08	2.15	2.81	4.34
	1.95	1.88	2.21	2.09	2.07	2.18	2.84	4.36

50min	1.92	2.06	2.03	2.06	1.93	2.23	2.63	4.63
	1.92	2.09	2.04	2.09	1.94	2.22	2.67	4.62
	1.92	2.07	2.06	2.10	2.08	2.22	2.70	4.64

60min	1.83	1.96	2.09	2.03	2.02	2.26	2.75	4.65
	1.84	1.99	2.12	2.05	2.03	2.25	2.80	4.65
	1.87	2.00	2.12	2.08	2.02	2.27	2.77	4.67

70min	2.13	2.22	2.22	2.09	2.23	2.30	2.90	4.32
	2.12	2.21	2.22	2.04	2.22	2.26	2.85	4.26
	2.15	2.24	2.24	2.07	2.23	2.28	2.86	4.26

80min	2.16	2.20	2.25	1.97	2.24	2.29	2.87	4.45
	2.15	2.21	2.23	1.94	2.23	2.28	2.84	4.37
	2.17	2.22	2.27	1.97	2.25	2.27	2.85	4.36

90min	2.29	2.13	2.26	2.16	2.19	2.28	3.08	4.30
	2.30	2.16	2.27	2.17	2.13	2.29	3.08	4.31
	2.28	2.19	2.30	2.20	2.16	2.31	3.14	4.38

sufA	H₂O₂
	less	1nM	10nM	100nM	1μM	10μM	100μM	1mM
10min	2.06	1.86	2.00	2.07	2.02	2.13	2.80	3.53
20min	2.26	2.21	2.21	2.08	2.02	2.15	2.86	4.24
30min	2.01	1.91	1.89	1.97	2.08	2.09	2.95	4.09
40min	1.95	1.89	2.18	2.07	2.07	2.14	2.78	4.34
50min	1.92	2.07	2.05	2.08	1.98	2.23	2.67	4.63
60min	1.85	1.98	2.11	2.05	2.02	2.26	2.77	4.65
70min	2.13	2.22	2.23	2.06	2.22	2.28	2.87	4.28
80min	2.16	2.21	2.25	1.96	2.24	2.28	2.85	4.39
90min	2.29	2.14	2.28	2.18	2.16	2.30	3.10	4.33

tetR

n=1

10min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	14.4	14.6	14.3	15.0	14.7	15.0	15.0	13.8
	14.5	14.8	14.3	15.0	14.5	15.0	14.8	13.8

20min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	15.2	15.2	15.0	16.0	14.9	14.7	14.8	15.1
	15.5	15.5	15.2	15.2	14.8	14.8	14.6	14.7

30min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	15.7	15.9	16.0	16.3	15.2	16.5	16.3	15.4
	16.0	16.2	16.3	16.4	15.3	16.6	16.5	15.6

40min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	13.3	15.2	14.4	14.5	14.8	15.6	15.5	15.3
	14.4	16.1	15.0	15.0	15.5	15.8	15.9	15.4

50min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	18.1	18.3	17.4	18.4	17.0	18.3	18.2	19.7
	18.3	18.6	17.8	18.7	17.4	18.5	18.5	20.2

60min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
60min	18.5	19.8	17.6	18.3	18.7	19.2	19.0	21.1

70min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	21.8	21.4	20.9	20.8	21.9	21.8	20.7	20.2
	21.9	21.3	20.7	20.8	21.9	21.5	20.6	20.1

80min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	22.9	25.2	22.6	22.1	21.6	22.8	22.1	20.8
	23.2	24.8	22.8	22.1	21.8	22.9	22.4	20.8

tetR	H₂O₂
	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
10min	14.5	14.7	14.3	15.0	15.0	15.0	14.9	13.8
20min	15.4	15.4	15.1	15.6	14.8	14.7	14.7	14.9
30min	15.9	16.1	16.1	16.4	15.3	16.5	16.4	15.5
40min	13.8	15.6	14.7	14.7	15.1	15.7	15.7	15.3
50min	18.2	18.5	17.6	18.6	17.2	18.4	18.4	19.9
60min	18.5	19.8	17.6	18.3	18.7	19.2	19.0	21.1
70min	21.9	21.4	20.8	20.8	21.9	21.6	20.7	20.1
80min	23.0	25.0	22.7	22.1	21.7	22.8	22.3	20.8

n=2

10min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	26.2	26.8	26.2	26.4	21.8	25.3	25.5	24.3
	26.2	26.,6	25.9	26.1	21.6	25.8	26.4	24.5
	26.1	26.8	26.1	26.2	22.0	26.3	26.4	24.8

20min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	28.9	28.1	24.5	26.8	26.8	27.7	25.7	25.0
	29.4	27.8	24.5	26.6	26.4	27.3	25.5	25.4
	29.8	28.0	24.9	27.1	26.7	27.7	25.9	25.8

30min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	28.7	28.0	2,9.0	28.1	27.0	27.4	27.9	28.4
	29.3	28.5	29.6	28.6	27.3	28.1	27.8	28.3
	29.7	28.8	30.0	28.9	27.9	28.3	28.2	28.2

40min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	26.6	26.9	19.8	27.5	29.3	27.1	26.3	28.5
	27.7	28.3	20.8	29.2	30.6	29.3	27.0	27.8
	28.2	29.0	20.9	29.2	31.2	29.5	27.3	28.0

50min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	32.9	29.3	29.6	29.6	31.5	31.6	31.3	30.6
	33.0	29.6	29.9	29.7	31.7	32.0	31.5	30.7
	33.0	30.0	30.2	30.2	32.0	32.3	31.8	30.9

60min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	35.0	29.1	28.8	26.9	26.8	27.1	30.0	27.9
	34.9	27.1	28.8	26.9	26.8	27.1	29.6	27.9
	34.7	27.8	28.6	26.9	26.4	27.4	29.6	27.9

70min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	37.9	34.5	33.6	33.0	32.4	32.4	31.1	33.7
	37.8	34.6	33.6	33.2	32.7	32.2	31.4	33.6
	38.3	35.3	34.1	33.5	33.1	33.1	31.9	34.1

80min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	38.5	35.2	36.7	35.9	38.1	36.9	32.5	34.6
	38.9	35.9	37.2	36.8	39.0	37.9	33.2	35.3
	39.4	36.3	37.3	37.0	39.1	38.1	33.4	35.4

90min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	40.1	38.6	37.9	36.7	37.1	37.1	38.3	34.4
	40.5	38.8	38.5	36.7	37.4	38.5	39.0	35.2
	40.7	39.2	39.0	37.3	37.5	38.6	39.4	35.7

tetR	H₂O₂
	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
10min	26.2	26.7	26.1	26.2	21.8	25.8	26.1	24.5
20min	29.4	27.9	24.6	26.8	26.6	27.6	25.7	25.4
30min	29.5	28.4	29.5	28.5	27.4	27.9	28.0	28.3
40min	33.0	29.6	29.9	29.8	31.7	32.0	31.5	30.7
50min	34.9	28.0	28.8	26.9	26.7	27.2	29.6	27.9
60min	38.0	34.8	33.8	33.2	32.7	32.5	31.5	33.8
70min	38.9	35.8	37.1	36.6	38.7	37.6	33.0	35.1
80min	40.4	38.9	38.4	36.9	37.3	38.2	38.9	35.1

n=3

10min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	28.3	30.6	28.5	27.8	29.6	33.6	31.8	24.1
	28.9	30.3	28.1	27.6	29.6	33.5	32.0	24.3
	28.9	30.3	28.0	27.4	29.5	33.5	32.1	24.2

20min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	33.5	30.2	30.2	31.2	33.2	31.4	31.3	31.2
	33.4	30.0	29.9	30.8	32.9	31.0	31.3	31.2
	33.4	30.4	29.9	30.9	32.8	31.3	31.5	31.2

30min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	33.5	34.0	34.8	25.8	30.3	35.7	31.0	32.1
	33.3	33.9	34.8	25.6	30.6	35.4	31.2	32.0
	33.2	34.1	34.9	26.1	30.6	36.3	31.6	32.0

40min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	30.9	34.0	36.1	34.2	34.1	31.5	31.6	32.9
	31.0	34.0	36.5	34.5	34.8	31.4	32.4	33.2
	31.5	34.4	37.1	35.4	35.4	32.4	33.1	33.7

50min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	34.2	35.3	36.0	35.6	35.0	35.6	37.3	36.4
	34.6	35.8	36.6	36.5	35.6	36.3	38.0	36.8
	35.1	36.1	37.0	36.9	36.0	36.8	38.3	37.0

60min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	37.4	35.5	33.0	37.0	38.2	34.7	36.7	34.1
	37.6	36.0	33.3	38.0	39.0	35.2	37.3	34.6
	38.3	36.5	33.7	38.0	39.5	35.6	37.9	35.2

70min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	41.5	42.8	40.0	40.7	39.4	37.9	37.2	36.7
	41.6	42.3	40.2	40.7	40.3	38.6	38.0	37.4
	41.8	42.5	40.1	40.9	40.5	39.1	38.4	37.7

80min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	41.4	39.8	43.4	42.8	41.9	42.3	43.2	39.6
	41.6	39.9	43.9	43.0	42.5	43.4	43.1	39.6
	41.9	40.2	44.3	43.3	42.9	44.0	43.6	39.9

90min	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
	41.8	39.7	40.2	39.2	39.9	38.9	37.0	35.7
	41.9	39.7	40.0	38.8	39.6	38.3	36.7	36.0
	41.9	39.7	40.3	38.8	39.8	38.6	37.1	35.9

tetR	H₂O₂
	less	1nM	10nM	100nM	1µM	10µM	100µM	1mM
10min	28.7	30.4	28.2	27.6	29.5	33.5	31.9	24.2
20min	33.4	30.2	30.0	31.0	32.9	31.2	31.3	31.2
30min	33.3	34.0	34.8	25.8	30.5	35.8	31.3	32.0
40min	31.1	34.1	36.6	34.7	34.7	31.8	32.4	33.3
50min	34.7	35.7	36.5	36.3	35.5	36.3	37.9	36.7
60min	41.6	42.5	40.1	40.7	40.1	38.5	37.9	37.3
70min	41.6	40.0	43.9	43.0	42.4	43.3	43.3	39.7
80min	41.9	39.7	40.2	39.0	39.8	38.6	37.0	35.9
90min	41.9	39.7	40.2	39.0	39.8	38.6	37.0	35.9

@@ Line 27: / Line 27: @@
 === K121013 ===
 :<TABLE BORDER="0"><TR>
-:<TD><TABLE BORDER="1"><TR>
 :<TD>n=1</TD></TR>
 :<TR>
+:<TD><TABLE BORDER="1"><TR>
 :<TD ROWSPAN="3">10min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
 :<TR>
@@ Line 556: / Line 556: @@
 :<TR>
 :<TD><TABLE BORDER="1"><TR>
-:<TD ROWSPAN="1">10min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1μM</TD><TD>10μM</TD><TD>100μM</TD><TD>1mM</TD></TR>
+:<TD ROWSPAN="4">10min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1μM</TD><TD>10μM</TD><TD>100μM</TD><TD>1mM</TD></TR>
 :<TR>
 :<TD>5.52</TD><TD>5.37</TD><TD>5.72</TD><TD>5.53</TD><TD>6.00</TD><TD>6.93</TD><TD>7.63</TD><TD>7.77</TD></TR>
@@ Line 1,227: / Line 1,227: @@
 :<TD>tetR</TD><TD>H<SUB>2</SUB>O<SUB>2</SUB></TD></TR>
 :<TR>
-:<TD>less</TD><TD>1nM</tD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
+:<TD></TD><TD>less</TD><TD>1nM</tD><TD>10nM</TD><TD>100nM</TD><TD>1µM</TD><TD>10µM</TD><TD>100µM</TD><TD>1mM</TD></TR>
 :<TR>
 :<TD>10min</TD><TD>28.7</TD><TD>30.4</TD><TD>28.2</TD><TD>27.6</TD><TD>29.5</TD><TD>33.5</TD><TD>31.9</TD><TD>24.2</TD></TR>

Team:KIT-Kyoto/Notebook/Promoters

From 2010.igem.org

Latest revision as of 21:48, 27 October 2010

Promoters

Contents

K121013

ahpC

sufA

tetR