Team:KIT-Kyoto/Notebook/Promoters

From 2010.igem.org

(Difference between revisions)
(→Contents)
(→ahpC)
Line 554: Line 554:
:[[Image:a22.jpg]]
:[[Image:a22.jpg]]
 +
:<TABLE BORDER="0"><TR>
 +
:<TD>n=3</TD></TR>
 +
:<TR>
 +
:<TD><TABLE BORDER="1"><TR>
 +
:<TD ROWSPAN="1">10min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1μM</TD><TD>10μM</TD><TD>100μM</TD><TD>1mM</TD></TR>
 +
:<TR>
 +
:<TD>5.519</TD><TD>5.366</TD><TD>5.722</TD><TD>5.526</TD><TD>6.003</TD><TD>6.93</TD><TD>7.826</TD><TD>7.772</TD></TR>
 +
:<TR>
 +
:<TD>5.628</TD><TD>5.463</TD><TD>5.693</TD><TD>5.491</TD><TD>5.948</TD><TD>6.931</TD><TD>7.672</TD><TD>7.709</TD></TR>
 +
:<TR>
 +
:<TD>5.555</TD><TD>5.419</TD><TD>5.69</TD><TD>5.439</TD><TD>5.948</TD><TD>6.97</TD><TD>7.796</TD><TD>7.799</TD></TR>
 +
:</TABLE></TD></TR>
 +
:<TR>
 +
:<TD><TABLE BORDER="1"><TR>
 +
:<TD ROWSPAN="4">20min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1μM</TD><TD>10μM</TD><TD>100μM</TD><TD>1mM</TD></TR>
 +
:<TR>
 +
:<TD>6.114</TD><TD>5.922</TD><TD>5.68</TD><TD>5.54</TD><TD>5.765</TD><TD>7.362</TD><TD>8.28</TD><TD>10</TD></TR>
 +
:<TR>
 +
:<TD>6.045</TD><TD>5.887</TD><TD>5.504</TD><TD>5.428</TD><TD>5.571</TD><TD>7.169</TD><TD>7.957</TD><TD>9.709</TD></TR>
 +
:<TR>
 +
:<TD>6.076</TD><TD>5.928</TD><TD>5.551</TD><TD>5.51</TD><TD>5.665</TD><TD>7.278</TD><TD>8.215</TD><TD>9.976</TD></TR>
 +
:</TABLE></TD></TR>
 +
:<TR>
 +
:<TD><TABLE BORDER="1"><TR>
 +
:<TD ROWSPAN="4">30min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1μM</TD><TD>10μM</TD><TD>100μM</TD><TD>1mM</TD></TR>
 +
:<TR>
 +
:<TD>6.02</TD><TD>5.805</TD><TD>6.293</TD><TD>6.025</TD><TD>6.297</TD><TD>7.475</TD><TD>8.558</TD><TD>10.12</TD></TR>
 +
:<TR>
 +
:<TD>6.027</TD><TD>5.805</TD><TD>6.261</TD><TD>6.028</TD><TD>6.286</TD><TD>7.421</TD><TD>8.522</TD><TD>9.961</TD></TR>
 +
:<TR>
 +
:<TD>6.116</TD><TD>5.871</TD><TD>6.314</TD><TD>6.102</TD><TD>6.349</TD><TD>7.561</TD><TD>8.628</TD><TD>9.952</TD></TR>
 +
:</TABLE></TD></TR>
 +
:<TR>
 +
:<TD><TABLE BORDER="1"><TR>
 +
:<TD ROWSPAN="4">40min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1μM</TD><TD>10μM</TD><TD>100μM</TD><TD>1mM</TD></TR>
 +
:<TR>
 +
:<TD>6.217</TD><TD>5.9</TD><TD>6.459</TD><TD>6.009</TD><TD>5.743</TD><TD>12.2</TD><TD>8.663</TD><TD>10.28</TD></TR>
 +
:<TR>
 +
:<TD>6.302</TD><TD>6.069</TD><TD>6.606</TD><TD>6.202</TD><TD>5.831</TD><TD>12.71</TD><TD>8.892</TD><TD>10.38</TD></TR>
 +
:<TR>
 +
:<TD>6.392</TD><TD>6.145</TD><TD>6.663</TD><TD>6.317</TD><TD>6.002</TD><TD>12.94</TD><TD>9.101</TD><TD>10.52</TD></TR>
 +
:</TABLE></TD></TR>
 +
:<TR>
 +
:<TD><TABLE BORDER="1"><TR>
 +
:<TD ROWSPAN="4">50min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1μM</TD><TD>10μM</TD><TD>100μM</TD><TD>1mM</TD></TR>
 +
:<TR>
 +
:<TD>6.59</TD><TD>6.478</TD><TD>6.767</TD><TD>6.743</TD><TD>6.917</TD><TD>8.139</TD><TD>9.588</TD><TD>12.06</TD></TR>
 +
:<TR>
 +
:<TD>6.724</TD><TD>6.662</TD><TD>6.953</TD><TD>6.901</TD><TD>7</TD><TD>8.334</TD><TD>9.738</TD><TD>12.09</TD></TR>
 +
:<TR>
 +
:<TD>6.859</TD><TD>6.801</TD><TD>7.103</TD><TD>7.051</TD><TD>7.109</TD><TD>8.47</TD><TD>10.04</TD><TD>12.34</TD></TR>
 +
:</TABLE></TD></TR>
 +
:<TR>
 +
:<TD><TABLE BORDER="1"><TR>
 +
:<TD ROWSPAN="4">60min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1μM</TD><TD>10μM</TD><TD>100μM</TD><TD>1mM</TD></TR>
 +
:<TR>
 +
:<TD>6.356</TD><TD>7.38</TD><TD>7.051</TD><TD>7.147</TD><TD>7.835</TD><TD>8.82</TD><TD>10.36</TD><TD>11.71</TD></TR>
 +
:<TR>
 +
:<TD>6.433</TD><TD>7.603</TD><TD>7.245</TD><TD>7.381</TD><TD>7.938</TD><TD>8.852</TD><TD>10.47</TD><TD>11.75</TD></TR>
 +
:<TR>
 +
:<TD>6.707</TD><TD>7.79</TD><TD>7.424</TD><TD>7.559</TD><TD>8.102</TD><TD>9.022</TD><TD>10.63</TD><TD>11.9</TD></TR>
 +
:</TABLE></TD></TR>
 +
:<TR>
 +
:<TD><TABLE BORDER="1"><TR>
 +
:<TD ROWSPAN="4">70min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1μM</TD><TD>10μM</TD><TD>100μM</TD><TD>1mM</TD></TR>
 +
:<TR>
 +
:<TD>9.194</TD><TD>9.162</TD><TD>9.247</TD><TD>9.085</TD><TD>8.83</TD><TD>11.65</TD><TD>12.54</TD><TD>12.95</TD></TR>
 +
:<TR>
 +
:<TD>9.395</TD><TD>9.308</TD><TD>9.208</TD><TD>9.154</TD><TD>8.706</TD><TD>11.53</TD><TD>12.44</TD><TD>13.27</TD></TR>
 +
:<TR>
 +
:<TD>9.605</TD><TD>9.564</TD><TD>9.367</TD><TD>9.358</TD><TD>8.712</TD><TD>11.78</TD><TD>12.62</TD><TD>13.42</TD></TR>
 +
:</TABLE></TD></TR>
 +
:<TR>
 +
:<TD><TABLE BORDER="1"><TR>
 +
:<TD ROWSPAN="4">80min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1μM</TD><TD>10μM</TD><TD>100μM</TD><TD>1mM</TD></TR>
 +
:<TR>
 +
:<TD>10.99</TD><TD>11.08</TD><TD>11.51</TD><TD>10.92</TD><TD>12.04</TD><TD>13.71</TD><TD>15.42</TD><TD>15.37</TD></TR>
 +
:<TR>
 +
:<TD>11.17</TD><TD>11.32</TD><TD>11.64</TD><TD>11.07</TD><TD>12.24</TD><TD>13.5</TD><TD>15.22</TD><TD>15.26</TD></TR>
 +
:<TR>
 +
:<TD>11.34</TD><TD>11.67</TD><TD>11.84</TD><TD>11.64</TD><TD>12.37</TD><TD>13.85</TD><TD>15.8</TD><TD>15.35</TD></TR>
 +
:</TABLE></TD></TR>
 +
:<TR>
 +
:<TD><TABLE BORDER="1"><TR>
 +
:<TD ROWSPAN="4">90min</TD><TD>less</TD><TD>1nM</TD><TD>10nM</TD><TD>100nM</TD><TD>1μM</TD><TD>10μM</TD><TD>100μM</TD><TD>1mM</TD></TR>
 +
:<TR>
 +
:<TD>16.03</TD><TD>17.62</TD><TD>14.97</TD><TD>14.62</TD><TD>15.31</TD><TD>16.37</TD><TD>17.11</TD><TD>17.3</TD></TR>
 +
:<TR>
 +
:<TD>16.2</TD><TD>17.81</TD><TD>15.08</TD><TD>14.67</TD><TD>15.46</TD><TD>16.44</TD><TD>17.19</TD><TD>17.45</TD></TR>
 +
:<TR>
 +
:<TD>16.39</TD><TD>18.07</TD><TD>15.34</TD><TD>14.96</TD><TD>15.7</TD><TD>16.64</TD><TD>17.38</TD><TD>17.66</TD></TR>
 +
:</TABLE></TD></TR></TABLE>
 +
 +
:[[Image:a3.jpg]]
=== sufA ===
=== sufA ===

Revision as of 12:58, 26 October 2010



Home > Notebook > Promoters

各種プロモーター活性

Contents

K121013
ahpC
sufA
tetR

Data

K121013

n=1
10minless1nM10nM100nM1µM10µM100µM1mM
0.3730.3100.2900.2930.3280.2720.3170.338
0.37200.31300.2740.2850.3250.2610.3000.327
20minless1nM10nM100nM1µM10µM100µM1mM
0.3460.3290.3210.2720.3660.2810.4790.324
0.3530.3430.3200.2750.3560.2800.4610.327
30minless1nM10nM100nM1µM10µM100µM1mM
0.3270.2890.3310.3560.3520.3530.3440.474
0.3390.2930.3330.3650.3580.3680.3400.467
40minless1nM10nM100nM1µM10µM100µM1mM
0.4390.4630.4160.4660.4610.3190.4750.419
0.4500.4680.4010.4880.4410.3140.4590.418
50minless1nM10nM100nM1µM10µM100µM1mM
0.3140.4450.3550.4520.3420.3530.4420.392
0.3100.4910.3700.4710.3380.3480.4240.392
60minless1nM10nM100nM1µM10µM100µM1mM
0.4680.4780.4590.4590.4460.3540.5150.420
70minless1nM10nM100nM1µM10µM100µM1mM
0.4520.44800.4530.5100.4140.4560.4160.435
0.4340.4340.5210.4000.4210.4280.4020.423
80minless1nM10nM100nM1µM10µM100µM1mM
0.4390.4580.4500.4170.5100.2480.4010.428
0.4270.4300.4260.4130.4770.2480.4010.432
K121013H2O2
less1nM10nM100nM1µM10µM100µM1mM
10min0.3730.3110.2810.2890.3260.2660.3070.333
20min0.3500.3360.3200.2740.3610.2800.4700.325
30min0.3330.2910.3320.3610.3550.3610.3420.471
40min0.4440.4650.4080.4770.4510.3160.4670.419
50min0.3120.4680.3630.4620.3400.3510.4330.392
60min0.4680.4780.4590.4590.4460.3540.5150.420
70min0.4430.4410.4870.4550.4170.4420.4090.429
80min0.4330.4440.4360.4150.4930.2480.4010.430
K12.jpg
n=2
10minless1nM10nM100nM1µM10µM100µM1mM
0.3240.2820.3270.3000.3290.3010.3060.362
0.3240.2860.3150.2990.3200.3060.3060.367
0.3210.2940.3130.3000.3270.3100.3150.367
20minless1nM10nM100nM1µM10µM100µM1mM
0.4640.4580.4360.4051.240.4400.3800.439
0.4380.4320.4000.3761.220.4250.3500.452
0.4190.4210.3990.3731.230.4130.3740.456
30minless1nM10nM100nM1µM10µM100µM1mM
0.3460.3530.4410.3330.4260.3850.3640.402
0.3530.3450.4320.3400.4090.3720.3680.416
0.3460.3460.4160.3420.4010.3660.3600.405
40minless1nM10nM100nM1µM10µM100µM1mM
0.3990.4610.4130.5410.4160.3950.5100.372
0.3700.4610.3960.5240.4030.3770.5060.377
0.3680.4500.3970.540.3960.3770.4800.379
50minless1nM10nM100nM1µM10µM100µM1mM
0.5000.4290.3550.4410.5660.4620.4500.414
0.4690.4070.3410.4050.5640.4210.4180.408
0.4340.3900.3400.3840.5750.4060.4090.413
60minless1nM10nM100nM1µM10µM100µM1mM
0.4050.3980.3950.3650.4420.4140.4030.411
0.3880.4050.3860.3650.4150.4060.4130.409
0.4030.4040.3890.3700.4050.4140.4170.405
70minless1nM10nM100nM1µM10µM100µM1mM
0.4260.6000.5570.4130.4540.4500.4340.387
0.4260.5450.5040.4210.4540.4620.4290.393
0.4360.5060.5000.4310.4650.4660.4460.404
80minless1nM10nM100nM1µM10µM100µM1mM
0.4570.4450.4560.4570.4640.4660.4500.417
0.4740.4580.4680.4710.4770.4840.4650.430
0.4740.4600.4700.4850.4810.4900.4680.416
90minless1nM10nM100nM1µM10µM100µM1mM
0.4690.4580.4910.4750.4830.4440.5330.446
0.4820.4640.4500.4810.4930.4430.5430.450
0.4910.4670.5100.4860.5060.450.5400.448
K121013H2O2
less1nM10nM100nM1µM10µM100µM1mM
10min0.3230.2870.3180.2990.3260.3060.3090.365
20min0.4400.4370.4120.3851.230.4260.3680.449
30min0.3480.3480.4290.3380.4120.3740.3640.408
40min0.4680.4090.3450.4100.5680.4300.4250.412
50min0.3990.4020.3900.3670.4200.4110.4110.408
60min0.4290.5500.5200.4220.4570.4590.4360.395
70min0.4690.4550.4650.4710.4740.4800.4610.421
80min0.4810.4630.4830.4810.4940.4460.5390.448
K22.jpg
n=3
10minless1nM10nM100nM1µM10µM100µM1mM
0.6740.6620.6840.6630.7230.6460.6670.682
0.6750.6670.6790.6670.7230.6550.6590.698
0.6790.6620.6820.6610.7310.6610.6470.693
20minless1nM10nM100nM1µM10µM100µM1mM
0.7150.7040.7120.7050.6950.6960.7681.06
0.7150.6900.6980.7060.6940.6950.7531.04
0.7140.7080.7050.6990.7090.7010.7361.04
30minless1nM10nM100nM1µM10µM100µM1mM
0.8090.7700.6640.7040.7590.6860.8440.726
0.7920.7600.6590.7150.7590.6920.8060.717
0.8110.7750.6810.7110.7580.6990.8160.716
40minless1nM10nM100nM1µM10µM100µM1mM
0.7680.7290.7130.7040.8000.7900.7760.742
0.7660.7260.7030.7100.7960.7880.7690.750
0.7720.7310.7070.7170.8020.7850.7730.759
50minless1nM10nM100nM1µM10µM100µM1mM
0.7220.7290.7550.7840.8140.8420.8380.918
0.7330.7320.7590.7860.8200.8480.8430.930
0.7400.7350.7670.7930.8250.8460.8380.936
60minless1nM10nM100nM1µM10µM100µM1mM
0.9010.8850.9810.8660.8340.8880.8440.968
0.9080.8970.9880.8730.8190.8950.8350.963
0.9140.8980.9850.8870.8430.8810.8330.956
70minless1nM10nM100nM1µM10µM100µM1mM
0.9420.9850.9670.9170.9140.9100.9700.932
0.9410.9720.9550.9000.9110.8970.9400.931
0.9420.9660.9570.9000.9030.8790.9530.941
80minless1nM10nM100nM1µM10µM100µM1mM
1.041.010.9941.021.031.010.9951.17
1.021.010.9871.010.9690.9950.9741.14
1.031.021.011.000.9760.9990.9791.14
90minless1nM10nM100nM1µM10µM100µM1mM
0.9791.011.051.021.040.9890.9331.12
0.9971.011.061.010.9951.010.9511.11
0.9821.011.071.041.021.000.9491.10
K121013H2O2
less1nM10nM100nM1µM10µM100µM1mM
10min0.6760.6630.6820.6640.7260.6540.6580.691
20min0.7150.7010.7050.7030.7000.6980.7521.05
30min0.8040.7680.6680.7100.7590.6930.8220.720
40min0.7690.7250.7070.7100.7990.7880.7720.750
50min0.7320.7320.7600.7870.8200.8450.8400.928
60min0.9420.9740.9600.9050.9090.8950.9550.935
70min1.031.020.9971.010.9901.000.9831.15
80min0.9861.011.061.021.020.9990.9441.11
90min0.9861.011.061.031.020.9990.9441.11
K32.jpg


ahpC

n=1
10minless1nM10nM100nM1µM10µM100µM1mM
6.366.556.645.957.199.8510.412.0
6.486.716.736.117.3410.211.012.4
6.526.726.786.157.4610.411.312.8
20minless1nM10nM100nM1µM10µM100µM1mM
7.826.917.316.927.2611.412.613.6
7.897.047.476.977.3711.412.913.9
30minless1nM10nM100nM1µM10µM100µM1mM
8.178.208.628.718.9711.313.616.1
8.328.308.798.838.9811.413.916.1
40minless1nM10nM100nM1µM10µM100µM1mM
8.378.759.208.528.8611.514.916.0
8.398.739.198.559.0511.915.316.2
50minless1nM10nM100nM1µM10µM100µM1mM
10.310.511.511.111.911.116.619.8
10.510.811.811.512.311.717.220.2
60minless1nM10nM100nM1µM10µM100µM1mM
11.913.313.013.313.816.019.422.5
12.113.713.313.514.016.319.823.1
70minless1nM10nM100nM1µM10µM100µM1mM
16.716.015.815.718.021.224.225.0
17.216.616.416.018.521.524.925.2
80minless1nM10nM100nM1µM10µM100µM1mM
21.227.022.223.423.525.628.931.3
2227.722.824.124.125.729.131.6
ahpCH2O2
less1nM10nM100nM1µM10µM100µM1mM
10min6.456.666.726.077.3310.210.912.4
20min7.866.987.396.947.3211.412.813.7
30min8.258.258.718.778.9811.313.816.1
40min8.388.749.208.538.9511.715.116.1
50min10.410.611.711.312.111.416.920.0
60min12.013.513.213.413.916.119.622.8
70min16.916.316.115.918.321.324.625.1
80min16.916.316.115.918.321.324.625.1
A12.jpg
n=2
10minless1nM10nM100nM1μM10μM100μM1mM
7.3337.587.4117.4678.2688.71310.8410.7
7.3177.537.2967.3488.2638.49211.311.55
7.3357.5217.2797.2948.2578.47811.5111.82
20minless1nM10nM100nM1μM10μM100μM1mM
8.058.1748.0318.1949.05310.7411.3613.23
8.1948.2688.1918.5359.2110.9211.6213.46
8.3438.3468.338.5739.30511.0711.8313.77
30minless1nM10nM100nM1μM10μM100μM1mM
8.6958.6468.7858.76410.6611.0912.4214.49
8.8888.7598.9668.83710.6910.8912.514.62
8.9618.9439.0978.90810.8211.0812.914.99
40minless1nM10nM100nM1μM10μM100μM1mM
9.3589.2028.8259.0810.4512.6913.2315.75
9.5959.3788.9619.20210.612.833.4415.86
9.6889.4039.1169.32410.7712.7713.6315.91
50minless1nM10nM100nM1μM10μM100μM1mM
10.1310.5210.310.1911.2512.8114.5917.94
10.4910.8610.6310.6511.5413.0914.8318.18
10.6911.0910.8410.8111.8113.3615.0818.36
60minless1nM10nM100nM1μM10μM100μM1mM
11.1611.9611.9312.5412.8214.4316.5319.15
11.2612.2312.1112.7113.0514.4816.6219.21
11.5412.4512.4113.0713.314.9216.9119.32
70minless1nM10nM100nM1μM10μM100μM1mM
13.2312.2913.6113.4415.8217.6118.3521.25
12.7612.2513.2813.4615.4217.3818.0120.47
12.9113.0514.1713.7416.1217.8918.8720.93
80minless1nM10nM100nM1μM10μM100μM1mM
16.2318.1317.8618.318.3320.9117.4622.93
16.1118.2417.918.5318.0520.5117.3422.31
16.4418.8818.419.2918.6921.118.1523.41
ahpCH2O2
less1nM10nM100nM1μM10μM100μM1mM
10min7.3283333337.5436666677.3286666677.3696666678.2626666678.56111.2166666711.35666667
20min8.1956666678.2626666678.1848.4349.18933333310.91 11.6033333313.48666667
30min8.8488.7826666678.9493333338.83633333310.7233333311.0212.6066666714.7
40min9.5479.3276666678.9673333339.20210.6066666712.7633333313.4333333315.84
50min10.4366666710.8233333310.5910.5511.5333333313.0866666714.8333333318.16
60min11.3212.2133333312.1512.7733333313.0566666714.6116.6866666719.22666667
70min12.9666666712.5313.6866666713.5466666715.7866666717.6266666718.4120.88333333
80min16.2618.4166666718.0533333318.7066666718.3566666720.8417.6522.88333333
A22.jpg
n=3
10minless1nM10nM100nM1μM10μM100μM1mM
5.5195.3665.7225.5266.0036.937.8267.772
5.6285.4635.6935.4915.9486.9317.6727.709
5.5555.4195.695.4395.9486.977.7967.799
20minless1nM10nM100nM1μM10μM100μM1mM
6.1145.9225.685.545.7657.3628.2810
6.0455.8875.5045.4285.5717.1697.9579.709
6.0765.9285.5515.515.6657.2788.2159.976
30minless1nM10nM100nM1μM10μM100μM1mM
6.025.8056.2936.0256.2977.4758.55810.12
6.0275.8056.2616.0286.2867.4218.5229.961
6.1165.8716.3146.1026.3497.5618.6289.952
40minless1nM10nM100nM1μM10μM100μM1mM
6.2175.96.4596.0095.74312.28.66310.28
6.3026.0696.6066.2025.83112.718.89210.38
6.3926.1456.6636.3176.00212.949.10110.52
50minless1nM10nM100nM1μM10μM100μM1mM
6.596.4786.7676.7436.9178.1399.58812.06
6.7246.6626.9536.90178.3349.73812.09
6.8596.8017.1037.0517.1098.4710.0412.34
60minless1nM10nM100nM1μM10μM100μM1mM
6.3567.387.0517.1477.8358.8210.3611.71
6.4337.6037.2457.3817.9388.85210.4711.75
6.7077.797.4247.5598.1029.02210.6311.9
70minless1nM10nM100nM1μM10μM100μM1mM
9.1949.1629.2479.0858.8311.6512.5412.95
9.3959.3089.2089.1548.70611.5312.4413.27
9.6059.5649.3679.3588.71211.7812.6213.42
80minless1nM10nM100nM1μM10μM100μM1mM
10.9911.0811.5110.9212.0413.7115.4215.37
11.1711.3211.6411.0712.2413.515.2215.26
11.3411.6711.8411.6412.3713.8515.815.35
90minless1nM10nM100nM1μM10μM100μM1mM
16.0317.6214.9714.6215.3116.3717.1117.3
16.217.8115.0814.6715.4616.4417.1917.45
16.3918.0715.3414.9615.716.6417.3817.66
A3.jpg

sufA

tetR

Retrieved from "http://2010.igem.org/Team:KIT-Kyoto/Notebook/Promoters"