"
Team:Freiburg Bioware/BioBricks/vector plasmid
From 2010.igem.org
(Difference between revisions)
VolkerMorath (Talk | contribs) |
VolkerMorath (Talk | contribs) |
||
| Line 13: | Line 13: | ||
<div style="width:935px; height:320px; "> | <div style="width:935px; height:320px; "> | ||
<div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> | <div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> | ||
| - | + | Vectorplasmid BioBricks allow it to reconstitute a vectorplasmid of your choice. Either the provided parts are assembled or an other Gene of Interest (GOI) can be integrated into an vectorplasmid. Beside the packaging capacity of 4.7 kbb, the possiblilities are virtually endless. <br> | |
| - | The Inverted Terminal Repeats (ITRs) are constructs form highly stable secondary structures that are recognized by the Rep proteins and function as a packiging signal. There for the ITRs have to flank the later | + | The Inverted Terminal Repeats (ITRs) are constructs form highly stable secondary structures that are recognized by the Rep proteins and function as a packiging signal. There for the ITRs have to flank the later vectorplasmid. <br> |
The Beta-globin and the human growth hormon polyadenylation sequence (hGH)are regulatory sequences that are ment to enhace the expression of the used GOI. | The Beta-globin and the human growth hormon polyadenylation sequence (hGH)are regulatory sequences that are ment to enhace the expression of the used GOI. | ||
| Line 49: | Line 49: | ||
<div style="width:935px; height:320px; "> | <div style="width:935px; height:320px; "> | ||
<div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> | <div style="float:left; width:500px; height:auto; margin: 0px 10px 0px 10px; text-align:justify;"> | ||
| - | + | For several applications users of the Virus Construction Kit will only want to insert their own GOI and not change the promoter and regulatory elements in a first experiment. For these applications we provide precursors of our vectorplasmid that reduce the required cloning on two steps. | |
</div> | </div> | ||
<div style="float:right; width:480px; height:auto; /*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/1/1c/Freiburg10_Vectorplasmid_cloning.png" width="480" | <div style="float:right; width:480px; height:auto; /*border: 1px solid black;*/"><img src="http://partsregistry.org/wiki/images/1/1c/Freiburg10_Vectorplasmid_cloning.png" width="480" | ||
Revision as of 12:37, 24 October 2010
Vectorplasmid BioBricks
Vectorplasmid BioBricks allow it to reconstitute a vectorplasmid of your choice. Either the provided parts are assembled or an other Gene of Interest (GOI) can be integrated into an vectorplasmid. Beside the packaging capacity of 4.7 kbb, the possiblilities are virtually endless.
The Inverted Terminal Repeats (ITRs) are constructs form highly stable secondary structures that are recognized by the Rep proteins and function as a packiging signal. There for the ITRs have to flank the later vectorplasmid.
The Beta-globin and the human growth hormon polyadenylation sequence (hGH)are regulatory sequences that are ment to enhace the expression of the used GOI.
The Inverted Terminal Repeats (ITRs) are constructs form highly stable secondary structures that are recognized by the Rep proteins and function as a packiging signal. There for the ITRs have to flank the later vectorplasmid.
The Beta-globin and the human growth hormon polyadenylation sequence (hGH)are regulatory sequences that are ment to enhace the expression of the used GOI.
Create your own Vectorplasmid with the PrecursorBricks
For several applications users of the Virus Construction Kit will only want to insert their own GOI and not change the promoter and regulatory elements in a first experiment. For these applications we provide precursors of our vectorplasmid that reduce the required cloning on two steps.
Assembled Vectorplasmid - ready for transfection
