Team:Freiburg Bioware/BioBricks/capsid coding

From 2010.igem.org

(Difference between revisions)
Line 7: Line 7:
-
<br><br><!---Basic Capsidcoding Constructs--->
+
<br><br><br><br><!---Basic Capsidcoding Constructs--->
<center><h1>Basic Capsidcoding Constructs</h1></center>
<center><h1>Basic Capsidcoding Constructs</h1></center>
<div style="width:935px; height:320px; ">
<div style="width:935px; height:320px; ">
Line 18: Line 18:
height="90px"/></a>
height="90px"/></a>
-
<br><br><!--- --->
+
<br><br><br><br><!--- --->
<center><h1>Hanna fragen...</h1></center>
<center><h1>Hanna fragen...</h1></center>
<div style="width:965px; height:320px; ">
<div style="width:965px; height:320px; ">
Line 31: Line 31:
-
<br><br><!---ViralBricks for Loop replacement--->
+
<br><br><br><br><!---ViralBricks for Loop replacement--->
<center><h1>ViralBricks for Loop replacement</h1></center>
<center><h1>ViralBricks for Loop replacement</h1></center>
<div style="width:935px; height:420px; ">
<div style="width:935px; height:420px; ">
Line 85: Line 85:
-
<br><br><!---Versions of the Rep-Vp123_p5-TATA-less--->
+
<br><br><br><br><!---Versions of the Rep-Vp123_p5-TATA-less--->
<center><h1>Versions of the Rep-Vp123_p5-TATA-less</h1></center>
<center><h1>Versions of the Rep-Vp123_p5-TATA-less</h1></center>
<div style="width:935px; height:320px; ">
<div style="width:935px; height:320px; ">
Line 122: Line 122:
height="110px"/></a>
height="110px"/></a>
-
<br><br><!---Versions of the pCMV_Vp123--->
+
<br><br><br><br><!---Versions of the pCMV_Vp123--->
<center><h1>Versions of the pCMV_Vp123 </h1></center>
<center><h1>Versions of the pCMV_Vp123 </h1></center>
<div style="width:935px; height:240px; ">
<div style="width:935px; height:240px; ">

Revision as of 10:46, 24 October 2010

Capsid Coding

<parttable>viral_vectors_aav_capsid</parttable>



Basic Capsidcoding Constructs





Hanna fragen...





ViralBricks for Loop replacement

ViralBricks are short nucleotide sequences derived from the gene of the viral coat proteins VP1-3 where they encode the two major surface exposed loops around the positions 453 and 587. Functional peptides are integrated in the loop tip which will be present on the surface of viral vectros that have been modified with the corresponding ViralBrick.

Different ViralBrick versions:

  • 453 integration site
  • 587 integration site
  • 587 integration site with HSPG knock-out

    • Different functional motifs:

  • Biotinylation Acceptor Peptide (BAP)
  • His-Affinity tag (His)
  • RGD-Motif for Integrin targeting
  • Z34C a antibody binding motif from proein A





    • Versions of the Rep-Vp123_p5-TATA-less





    Versions of the pCMV_Vp123

    The different versions of pCMV_VP123 are ment for the production of mosaic viruses by transfecting this plamid together with [AAV2]-Rep-VP123_P5-TATA-less. The composite part pCMV_VP123 contains the ORF of the Viral Coat Proteins VP123 under the control of the control of the Cytomegalovirus promoter. By providing modified and unmodified viral coat proteins the resulting viral vector will be a chimera. The ratio of modified to unmodified viral coat proteins will depend on the number of plasmids a cell was transfected with and has impect on the functionality of the viral vector.

    Retrieved from "http://2010.igem.org/Team:Freiburg_Bioware/BioBricks/capsid_coding"