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Team:British Columbia/Notebook Biofilm
From 2010.igem.org
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<h3>Protocol</h3> | <h3>Protocol</h3> | ||
| - | <p>Day 1</p> | + | <p><b>Day 1</b></p> |
| + | <p>1. PIck an isolated colony from a Trypticase Soy Agar (TSA) plate and incubate in 5 mL of Trypticase Soy Broth (TSB) without shaking overnight.</p> | ||
| + | <p><b>Day 2</b></p | ||
| + | <p>1. Vortex the overnight culture and dilute 30 uL of culture by 1:100 in a 1% glucose solution diluted with TSB. | ||
| + | 2. akjhskjh</p> | ||
<br></br> | <br></br> | ||
</div> <!-- end SubWrapper --> | </div> <!-- end SubWrapper --> | ||
Revision as of 08:35, 23 October 2010
Protocol
Day 1
1. PIck an isolated colony from a Trypticase Soy Agar (TSA) plate and incubate in 5 mL of Trypticase Soy Broth (TSB) without shaking overnight.
Day 2
1. Vortex the overnight culture and dilute 30 uL of culture by 1:100 in a 1% glucose solution diluted with TSB. 2. akjhskjh
