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Team:Tokyo Tech/Parts
From 2010.igem.org
New parts which were characterized and worked as designed
Apple Reporter
| Name | Part type | Registry Link |
|---|---|---|
| MpAAT1 | Generator | <partinfo>BBa_K395602</partinfo> |
| rbs-crtW | Translational unit | <partinfo>BBa_K395705</partinfo> |
| pBad/araC-crtZW | Generator | <partinfo>BBa_K395706</partinfo> |
Artificial Cooperation System
| Name | Part type | Registry Link |
|---|---|---|
| (PluxR rep weak-GFP) improved | Reporter | <partinfo>BBa_K395105</partinfo> |
| PluxR act-Cm | signalling | <partinfo>BBa_K395162</partinfo> |
"Wolfcoli"zation
| Name | Part type | Registry Link |
|---|---|---|
| PompC(CB)-GFP improved | Reporter | <partinfo>BBa_K395305</partinfo> |
| PompC(CS1)-GFP | Reporter | <partinfo>BBa_K395306</partinfo> |
New parts which were characterized and did not worked as designed
"Wolfcoli"zation
| Name | Part type | Registry Link |
|---|---|---|
| PompC(C)-GFP | Reporter | <partinfo>BBa_K395304</partinfo> |
| PompC(WT)-GFP | Reporter | <partinfo>BBa_K395307</partinfo> |
Existing parts which were characterized through our activity
Apple Reporter
| Name | Part type | Registry Link |
|---|---|---|
| crtZ (beta-carotene hydroxylase) coding sequence | Coding | <partinfo>BBa_I742157</partinfo> |
| crtZ (beta-carotene hydroxylase) with native rbs | Translational Unit | <partinfo>BBa_I742158</partinfo> |
In order to characterize these parts, following 3 improved parts were constructed.
(Name,Parts,registry Number)
(placIQ-crtZ ,Generator,<partinfo>BBa_K395701</partinfo>)
(pBad/araC-crtZ ,Generator,<partinfo>BBa_K395702</partinfo>)
(pBad/araC-crtZ-crtEBIY ,Generator,<partinfo>BBa_K395704</partinfo>)
“Wolfcoli”zation
| Name | Part type | Registry Link |
|---|---|---|
| LacI-Mutant | Coding | <partinfo>BBa_K082005</partinfo> |
| LacIM1-B0015 | Composite | <partinfo>BBa_K082016</partinfo> |
| B0034-LacIM1-B0015 | Generator | <partinfo>BBa_K082026</partinfo> |
In order to characterize these parts, following 2 improved parts were constructed.
(Name,Parts,registry Number)
(Pbad/araC-lacIM1 ,Composite,<partinfo>BBa_K395401</partinfo>)
(Pbad/araC-lacI ,Composite,<partinfo>BBa_K395402</partinfo>)
Artificial Cooperation System
| Name | Part type | Registry Link |
|---|---|---|
| PluxR rep | Regulatory | <partinfo>BBa_R0061</partinfo> |
In order to characterize this part, following improved part was constructed.
(Name,Parts,registry Number)
(GFP reporter repressed by LuxR and 3OC6HSL ,Reporter,<partinfo>BBa_K395101</partinfo>)
| Name | Part type | Registry Link |
|---|---|---|
| PluxR act | Regulatory | <partinfo>BBa_R0062</partinfo> |
In order to characterize this part, following part was constructed.
(Name,Parts,registry Number)
(GFP reporter activated by LuxR and 3OC6HSL,Reporter,<partinfo>BBa_K395100</partinfo>)
All of the parts we registered
<groupparts>iGEM010 Tokyo_Tech</groupparts>
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