Team:Tokyo Tech/Parts

From 2010.igem.org

iGEM Tokyo Tech 2010 "E.coli with Humanity"


New parts which were characterized and worked as designed

Apple Reporter

NamePart typeRegistry Link
MpAAT1GeneratorBBa_K395602
rbs-crtWTranslational unitBBa_K395705
pBad/araC-crtZWGeneratorBBa_K395706

Artificial Cooperation System

NamePart typeRegistry Link
(PluxR rep weak-GFP) improvedReporterBBa_K395105
PluxR act-CmsignallingBBa_K395162

"Wolfcoli"zation

NamePart typeRegistry Link
PompC(CB)-GFP improvedReporterBBa_K395305
PompC(CS1)-GFPReporterBBa_K395306

New parts which were characterized and did not worked as designed

"Wolfcoli"zation

NamePart typeRegistry Link
PompC(C)-GFP ReporterBBa_K395304
PompC(WT)-GFP ReporterBBa_K395307

Existing parts which were characterized through our activity

Apple Reporter

NamePart typeRegistry Link
crtZ (beta-carotene hydroxylase) coding sequence CodingBBa_I742157
crtZ (beta-carotene hydroxylase) with native rbs Translational UnitBBa_I742158

In order to characterize these parts, following 3 improved parts were constructed.


(Name,Parts,registry Number)

(placIQ-crtZ ,Generator,BBa_K395701)

(pBad/araC-crtZ ,Generator,BBa_K395702)

(pBad/araC-crtZ-crtEBIY ,Generator,BBa_K395704)



“Wolfcoli”zation

NamePart typeRegistry Link
LacI-Mutant CodingBBa_K082005
LacIM1-B0015CompositeBBa_K082016
B0034-LacIM1-B0015GeneratorBBa_K082026

In order to characterize these parts, following 2 improved parts were constructed.


(Name,Parts,registry Number)

(Pbad/araC-lacIM1 ,Composite,BBa_K395401)

(Pbad/araC-lacI ,Composite,BBa_K395402)


Artificial Cooperation System

NamePart typeRegistry Link
PluxR repRegulatoryBBa_R0061

In order to characterize this part, following improved part was constructed.


(Name,Parts,registry Number)

(GFP reporter repressed by LuxR and 3OC6HSL ,Reporter,BBa_K395101)
NamePart typeRegistry Link
PluxR actRegulatoryBBa_R0062

In order to characterize this part, following part was constructed.


(Name,Parts,registry Number)

(GFP reporter activated by LuxR and 3OC6HSL,Reporter,BBa_K395100)

All of the parts we registered

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