Team:Tokyo Tech/Parts

From 2010.igem.org

iGEM Tokyo Tech 2010 "E.coli with Humanity"


New parts which were characterized and worked as designed

Apple Reporter

NamePart typeRegistry Link
MpAAT1Generator<partinfo>BBa_K395602</partinfo>
rbs-crtWTranslational unit<partinfo>BBa_K395705</partinfo>
pBad/araC-crtZWGenerator<partinfo>BBa_K395706</partinfo>

Artificial Cooperation System

NamePart typeRegistry Link
(PluxR rep weak-GFP) improvedReporter<partinfo>BBa_K395105</partinfo>
PluxR act-Cmsignalling<partinfo>BBa_K395162</partinfo>

"Wolfcoli"zation

NamePart typeRegistry Link
PompC(CB)-GFP improvedReporter<partinfo>BBa_K395305</partinfo>
PompC(CS1)-GFPReporter<partinfo>BBa_K395306</partinfo>

New parts which were characterized and did not worked as designed

"Wolfcoli"zation

NamePart typeRegistry Link
PompC(C)-GFP Reporter<partinfo>BBa_K395304</partinfo>
PompC(WT)-GFP Reporter<partinfo>BBa_K395307</partinfo>

Existing parts which were characterized through our activity

Apple Reporter

NamePart typeRegistry Link
crtZ (beta-carotene hydroxylase) coding sequence Coding<partinfo>BBa_I742157</partinfo>
crtZ (beta-carotene hydroxylase) with native rbs Translational Unit<partinfo>BBa_I742158</partinfo>

In order to characterize these parts, following 3 improved parts were constructed.


(Name,Parts,registry Number)

(placIQ-crtZ ,Generator,<partinfo>BBa_K395701</partinfo>)

(pBad/araC-crtZ ,Generator,<partinfo>BBa_K395702</partinfo>)

(pBad/araC-crtZ-crtEBIY ,Generator,<partinfo>BBa_K395704</partinfo>)



“Wolfcoli”zation

NamePart typeRegistry Link
LacI-Mutant Coding<partinfo>BBa_K082005</partinfo>
LacIM1-B0015Composite<partinfo>BBa_K082016</partinfo>
B0034-LacIM1-B0015Generator<partinfo>BBa_K082026</partinfo>

In order to characterize these parts, following 2 improved parts were constructed.


(Name,Parts,registry Number)

(Pbad/araC-lacIM1 ,Composite,<partinfo>BBa_K395401</partinfo>)

(Pbad/araC-lacI ,Composite,<partinfo>BBa_K395402</partinfo>)


Artificial Cooperation System

NamePart typeRegistry Link
PluxR repRegulatory<partinfo>BBa_R0061</partinfo>

In order to characterize this part, following improved part was constructed.


(Name,Parts,registry Number)

(GFP reporter repressed by LuxR and 3OC6HSL ,Reporter,<partinfo>BBa_K395101</partinfo>)
NamePart typeRegistry Link
PluxR actRegulatory<partinfo>BBa_R0062</partinfo>

In order to characterize this part, following part was constructed.


(Name,Parts,registry Number)

(GFP reporter activated by LuxR and 3OC6HSL,Reporter,<partinfo>BBa_K395100</partinfo>)

All of the parts we registered

<groupparts>iGEM010 Tokyo_Tech</groupparts>


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