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BIOTEC Dresden/Notepad/8 September 2010
From 2010.igem.org
Parts Assembly
Ligation of the digested samples from 07.09.2010 was done. A 1:1 and 1:3 ligation was done. The ligation mix was transformed by electroporation and plated and left overnight at 37 degrees.
4a, 9b, 14f, 20f, 21f
The concentration of all the plasmid backbones containing chloramphenicol was measured by nanodrop.
Fusion Protein
A new ligation with aiiA and protein A was set up. This time only the molar ratio 1:1 was used for triple ligation. For the double ligations again 1:1 and 1:3 was used. Ligation reactions were incubated for 1 hour at room temperature. The whole reaction was later used for electroporation.
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