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BIOTEC Dresden/Notepad/25 September 2010
From 2010.igem.org
Parts Assembly
The PCR step from 21.09.2010 was repeated for the following parts and the plasmid backbone.
4a, 14f, 20f, 21f
The PCR products were run on an agarose gel for gel purification and the purified samples were nanodropped. A restriction digest of these samples were done.
4a, 14f, 20f, 21f
The digest was purified by PCR purification and a 1:3 and 1:1 ligation was done for the digested samples. The ligation mix was tranformed by electroporation and plated and left for overnight incubation.
Fusion Protein
A Miniprep and glycerol stocks from the overnight culturesof the fusion protein were prepared. A control digest to varify the correct insert was carried out. Unfortunatley not enough DNA was used to confirm the insertion.
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