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BIOTEC Dresden/Notepad/21 August 2010
From 2010.igem.org
Restriction digest of the PCR purified samples from 20.08.2010 was done with DpnI for 2 hours. The digested samples were purified and then run on an agarose gel to check for the specific bands. The concentration was measured.
Different primers (ASP and SP - 100 uM) were used for the PCR amplification of the backbone. The samples were then PCR purified and then run on an agarose gel and nanodropped.
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