Team:KIT-Kyoto/Protocol
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<tr><td>DNA溶液</td><td>DNA量が1 μgになるように調整する</td></tr> | <tr><td>DNA溶液</td><td>DNA量が1 μgになるように調整する</td></tr> | ||
<tr><td>制限酵素 | <tr><td>制限酵素 | ||
- | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=ECO-111&us_id=&us_pwd=&bun_id=0200100100410&iv_trademark=ECO-1&kskh_kbn=01&ksk_jkn=ECO-1<I>Eco</I>R I] | + | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=ECO-111&us_id=&us_pwd=&bun_id=0200100100410&iv_trademark=ECO-1&kskh_kbn=01&ksk_jkn=ECO-1 <I>Eco</I>R I] |
- | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=XBA- | + | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=XBA-101W&us_id=&us_pwd=&bun_id=0200100100970&iv_trademark=XBA-1&kskh_kbn=01&ksk_jkn=XBA-1 <I>Xba</I> I] |
- | 101W&us_id=&us_pwd=&bun_id=0200100100970&iv_trademark=XBA-1&kskh_kbn=01&ksk_jkn=XBA-1<I>Xba</I> I] | + | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=PST-111&us_id=&us_pwd=?us_id=&bun_id=0200100100760&iv_trademark=PST-1&kskh_kbn=01&ksk_jkn=PST-1 <I>Pst</I> I] |
- | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=PST- | + | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=SPE-101&us_id=&us_pwd=?us_id=&bun_id=0200100100900&iv_trademark=SPE-1&kskh_kbn=01&ksk_jkn=SPE-1 <I>Spe</I> I]</td><td>3ユニットになるように調整する</td></tr> |
- | 111&us_id=&us_pwd=?us_id=&bun_id=0200100100760&iv_trademark=PST-1&kskh_kbn=01&ksk_jkn=PST-1<I>Pst</I> I] | + | |
- | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=SPE- | + | |
- | 101&us_id=&us_pwd=?us_id=&bun_id=0200100100900&iv_trademark=SPE-1&kskh_kbn=01&ksk_jkn=SPE-1<I>Spe</I> I]</td><td>3ユニットになるように調整する</td></tr> | + | |
<tr><td>ddH2O</td><td>全量が20 μlになるように調整する</td></tr> | <tr><td>ddH2O</td><td>全量が20 μlになるように調整する</td></tr> | ||
<tr><td> </td><td>全量 20 μl</td></tr></table></td><td> </td><td><table border=1 width="300px"> | <tr><td> </td><td>全量 20 μl</td></tr></table></td><td> </td><td><table border=1 width="300px"> | ||
<tr><td width="100px">10 x Buffer | <tr><td width="100px">10 x Buffer | ||
- | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=H-BUFF&us_id=&us_pwd=&bun_id=020010020&iv_trademark=BUF-1&kskh_kbn=01&ksk_jkn=BUF- | + | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=H-BUFF&us_id=&us_pwd=&bun_id=020010020&iv_trademark=BUF-1&kskh_kbn=01&ksk_jkn=BUF-1 H Buffer] |
- | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=M- | + | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=M-BUFF&us_id=&us_pwd=&bun_id=020010020&iv_trademark=BUF-1 M Buffer]</td><td width="200px">2 μl</td></tr> |
- | BUFF&us_id=&us_pwd=&bun_id=020010020&iv_trademark=BUF- | + | |
<tr><td>DNA solution</td><td>Adjust for the amount of DNA to become 1 μg.</td></tr> | <tr><td>DNA solution</td><td>Adjust for the amount of DNA to become 1 μg.</td></tr> | ||
<tr><td>Restriction enzyme | <tr><td>Restriction enzyme | ||
- | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=ECO- | + | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=ECO-111&us_id=&us_pwd=&bun_id=0200100100410&iv_trademark=ECO-1&kskh_kbn=01&ksk_jkn=ECO-1 <I>Eco</I>R I] |
- | 111&us_id=&us_pwd=&bun_id=0200100100410&iv_trademark=ECO-1&kskh_kbn=01&ksk_jkn=ECO-1<I>Eco</I>R I] | + | |
:[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=XBA-101W&us_id=&us_pwd=&bun_id=0200100100970&iv_trademark=XBA-1&kskh_kbn=01&ksk_jkn=XBA-1<I>Xba</I> I] | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=XBA-101W&us_id=&us_pwd=&bun_id=0200100100970&iv_trademark=XBA-1&kskh_kbn=01&ksk_jkn=XBA-1<I>Xba</I> I] | ||
- | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=PST-111&us_id=&us_pwd=?us_id=&bun_id=0200100100760&iv_trademark=PST-1&kskh_kbn=01&ksk_jkn=PST-1<I>Pst</I> I] | + | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=PST-111&us_id=&us_pwd=?us_id=&bun_id=0200100100760&iv_trademark=PST-1&kskh_kbn=01&ksk_jkn=PST-1 <I>Pst</I> I] |
- | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=SPE-101&us_id=&us_pwd=?us_id=&bun_id=0200100100900&iv_trademark=SPE-1&kskh_kbn=01&ksk_jkn=SPE-1<I>Spe</I> I]</td><td>Adjust to become 3 units.</td></tr> | + | :[http://www.bio.toyobo.co.jp/bio01/cgi-bin/catalog_shohin_index?item=SPE-101&us_id=&us_pwd=?us_id=&bun_id=0200100100900&iv_trademark=SPE-1&kskh_kbn=01&ksk_jkn=SPE-1 <I>Spe</I> I]</td><td>Adjust to become 3 units.</td></tr> |
<tr><td>ddH2O</td><td>Adjust to become total 20 μl.</td></tr> | <tr><td>ddH2O</td><td>Adjust to become total 20 μl.</td></tr> | ||
<tr><td> </td><td>20 μl system</td></tr></table></td></tr> | <tr><td> </td><td>20 μl system</td></tr></table></td></tr> |
Revision as of 20:18, 23 October 2010
Language : English / Japanese |
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