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Team:IvyTech-South Bend/Notebook
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{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=06 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=07 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=08 }} | {{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=06 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=07 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=08 }} | ||
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{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=09 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=10 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=11 }} | {{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=09 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=10 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=11 }} | ||
== 8/24/10 == | == 8/24/10 == | ||
| + | |||
Today we will be pouring new plates for streaking new transformed cells. | Today we will be pouring new plates for streaking new transformed cells. | ||
| Line 62: | Line 59: | ||
| - | + | We will be running a gel to determine if our DNA sample was successfully electroplated. | |
== 8/27/10 == | == 8/27/10 == | ||
| - | + | Today we will be electroplating part KBBa_3131010 | |
| - | + | ||
== 8/31/10 == | == 8/31/10 == | ||
| - | + | Results of transformation - the plates had growth but (no) distinct colony pattern. | |
| - | + | ||
== 8/31/10 == | == 8/31/10 == | ||
| - | + | After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH | |
| - | + | ||
== 9/1/10 == | == 9/1/10 == | ||
| - | + | Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock. | |
== 9/2/10 == | == 9/2/10 == | ||
| - | |||
Results from streaking – | Results from streaking – | ||
== 9/2/10 == | == 9/2/10 == | ||
| - | |||
Protocol For Making LB-Broth/Agar | Protocol For Making LB-Broth/Agar | ||
| - | |||
| - | |||
== 9/3/10 == | == 9/3/10 == | ||
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Today I’m finishing making the LB/Agar from yesterday. | Today I’m finishing making the LB/Agar from yesterday. | ||
== 9/9/10 == | == 9/9/10 == | ||
| - | |||
Due to conflict we will be changing from E Coli to yeast. | Due to conflict we will be changing from E Coli to yeast. | ||
== 9/10/10 == | == 9/10/10 == | ||
| - | |||
Lux Casette Right Promoter BBa_I1051 | Lux Casette Right Promoter BBa_I1051 | ||
| Line 112: | Line 99: | ||
== 9/14/10 == | == 9/14/10 == | ||
| - | + | Today we found growth from our Electroporated E – Coli parts | |
| - | + | ||
== 9/15/10 == | == 9/15/10 == | ||
| - | + | Today we will be extracting DNA from out electroporated cells/T9002 | |
| - | + | ||
| - | + | ||
| - | + | ||
== 9/16/10 == | == 9/16/10 == | ||
| - | + | Today we will wash | |
| - | + | ||
== 9/17/10 == | == 9/17/10 == | ||
| - | + | Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work! | |
| - | + | ||
== 9/21/10 == | == 9/21/10 == | ||
| - | + | Today we will electroporate Agrobacterium with part T9002 using protocol from pg 22 – 23 | |
| - | + | ||
== 9/22/10 == | == 9/22/10 == | ||
| - | + | Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample | |
| - | + | ||
== 9/22/10 == | == 9/22/10 == | ||
| - | + | The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens | |
| - | + | ||
== 9/23/10 == | == 9/23/10 == | ||
| - | + | Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces. | |
| - | + | ||
==9/24/10 == | ==9/24/10 == | ||
| - | + | Today I’m testing absorbance of our trials @ 395nm | |
| - | + | ||
== 9/28/10 == | == 9/28/10 == | ||
| - | + | Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10 | |
| - | + | ||
| - | + | ||
Revision as of 14:18, 12 October 2010
Contents |
Notebook
| June | ||||||
| M | T | W | T | F | S | S |
| 1 | 2 | 3 | 4 | 5 | 6 | |
| 7 | 8 | 9 | 10 | 11 | 12 | 13 |
| 14 | 15 | 16 | 17 | 18 | 19 | 20 |
| 21 | 22 | 23 | 24 | 25 | 26 | 27 |
| 28 | 29 | 30 | ||||
| July | ||||||
| M | T | W | T | F | S | S |
| 1 | 2 | 3 | 4 | |||
| 5 | 6 | 7 | 8 | 9 | 10 | 11 |
| 12 | 13 | 14 | 15 | 16 | 17 | 18 |
| 19 | 20 | 21 | 22 | 23 | 24 | 25 |
| 26 | 27 | 28 | 29 | 30 | 31 | |
| August | ||||||
| M | T | W | T | F | S | S |
| 1 | ||||||
| 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| 9 | 10 | 11 | 12 | 13 | 14 | 15 |
| 16 | 17 | 18 | 19 | 20 | 21 | 22 |
| 23 | 24 | 25 | 26 | 27 | 28 | 29 |
| 30 | 31 | |||||
| September | ||||||
| M | T | W | T | F | S | S |
| 1 | 2 | 3 | 4 | 5 | ||
| 6 | 7 | 8 | 9 | 10 | 11 | 12 |
| 13 | 14 | 15 | 16 | 17 | 18 | 19 |
| 20 | 21 | 22 | 23 | 24 | 25 | 26 |
| 27 | 28 | 29 | 30 | |||
| October | ||||||
| M | T | W | T | F | S | S |
| 1 | 2 | 3 | ||||
| 4 | 5 | 6 | 7 | 8 | 9 | 10 |
| 11 | 12 | 13 | 14 | 15 | 16 | 17 |
| 18 | 19 | 20 | 21 | 22 | 23 | 24 |
| 25 | 26 | 27 | 28 | 29 | 30 | 31 |
| November | ||||||
| M | T | W | T | F | S | S |
| 1 | 2 | 3 | 4 | 5 | 6 | 7 |
| 8 | 9 | 10 | 11 | 12 | 13 | 14 |
| 15 | 16 | 17 | 18 | 19 | 20 | 21 |
| 22 | 23 | 24 | 25 | 26 | 27 | 28 |
| 29 | 30 | |||||
8/24/10
Today we will be pouring new plates for streaking new transformed cells.
8/27/10
We will be running a gel to determine if our DNA sample was successfully electroplated.
8/27/10
Today we will be electroplating part KBBa_3131010
8/31/10
Results of transformation - the plates had growth but (no) distinct colony pattern.
8/31/10
After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH
9/1/10
Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock.
9/2/10
Results from streaking –
9/2/10
Protocol For Making LB-Broth/Agar
9/3/10
Today I’m finishing making the LB/Agar from yesterday.
9/9/10
Due to conflict we will be changing from E Coli to yeast.
9/10/10
Lux Casette Right Promoter BBa_I1051
9/14/10
Today we found growth from our Electroporated E – Coli parts
9/15/10
Today we will be extracting DNA from out electroporated cells/T9002
9/16/10
Today we will wash
9/17/10
Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work!
9/21/10
Today we will electroporate Agrobacterium with part T9002 using protocol from pg 22 – 23
9/22/10
Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample
9/22/10
The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens
9/23/10
Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces.
9/24/10
Today I’m testing absorbance of our trials @ 395nm
9/28/10
Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10
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