IGEM Part #s and Uses
BBa_T9002 – Producer Controler Device–Plate2 Well 9A
BBa_I732017 – LacZ with our GFP 1—Plate 2 Well 3K
BBa_F2620– LuxR with terminator– Plate 2 Well 6E
BBa_I13522 –Tet Prop with GFP—Plate 2 Well 8A
BBa_T13521 Red Flouro tet RFP—Plate 2 Well 6O
BBa_pSB1C3—Plasmid—Plate 1 Well 3A
BBa_pSB1T3—Tetracycline resistant plasmid—Plate 1 Well 7A
BBa_pSB1AT3--high copy number plasmid carrying ampicillin and tetracycline resistance--Plate 1 Well 13A
Richard Lab:Electroporation of E. coli
Usage and extraction
Today we will be pouring new plates for streaking new transformed cells.
We will be running a gel to determine if our DNA sample was successfully electroplated.
Today we will be electroplating part KBBa_3131010
Results of transformation - the plates had growth but (no) distinct colony pattern.
After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH
Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock.
Results from streaking –
Protocol For Making LB-Broth/Agar
Today I’m finishing making the LB/Agar from yesterday.
Due to conflict we will be changing from E Coli to yeast.
Lux Casette Right Promoter BBa_I1051
Today we found growth from our Electroporated E – Coli parts
Today we will be extracting DNA from out electroporated cells/T9002 B-galactosidase Assay
Today we will wash
Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work!
Today we will electroporate Agrobacterium with part T9002
Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample
The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens
Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces.
Today I’m testing absorbance of our trials @ 395nm
Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10
LacZ without GFP also with RBS and terminator
Electroporating 2 parts with E-coli bacteria
LB-Lennox Broth & Electroporation
hold until Friday
Using openwetware.org protocol
BBL Trypticase soy Broth and Agar
Protocol-- Electroporation man for E-Coli
High Efficiency Electrotransformation of E.coli
DNA from BlueGuy
Final steps pack and ship
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