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From 2010.igem.org

Today we will wash our electrocomp grown t/pos bacteria with 1.2 mL Glycerol then electroporate

Following protocol from page 15

First prof. T sterilized the glycerol passing it through the .2 micron filter into 50 mL sterile centrifuge tube 1 –

then we added 1 mL 10 percent Glycerol to suspended cells 2 –

then centrifuged at 1000 rpm for 10 min -

then removed supernate with BR 1000 micropipettor - then add 1 mL 10 percent Glycerol then repeat steps 1 & 2 “4” times

then remove sup. and freeze cells at -80 degrees C freezer.

- Today we also set up a new IGem work area in back lab

look at pic