Team:IvyTech-South Bend/Notebook

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<img src="https://static.igem.org/mediawiki/2010/2/2d/IvyTech-South_Bend_logo.png" align="left"><img src="https://static.igem.org/mediawiki/2010/7/76/Igem1.png" align="left"><a href="https://2010.igem.org/Team:IvyTech-South_Bend/Team">
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<img src="https://static.igem.org/mediawiki/2010/d/d7/2010.png"align="left"></a><img src="https://static.igem.org/mediawiki/2010/a/a3/Sb.png" align="left">
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<a href="https://2010.igem.org/Team:IvyTech-South_Bend"> <img src="https://static.igem.org/mediawiki/2010/f/f3/Home1.png" width=12%></a> 
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<a href="https://2010.igem.org/Team:IvyTech-South_Bend/Team"><img src="https://static.igem.org/mediawiki/2010/3/32/Team.png" width=12%></a>  
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<a href="https://2010.igem.org/Talk:Team:IvyTech-South_Bend/Notebook">Discussion</a>
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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This is a template page. READ THESE INSTRUCTIONS.
 
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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|You can write a background of your team here. Give us a background of your team, the members, etc. Or tell us more about something of your choosing.
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|[[Image:IvyTech-South_Bend_logo.png|200px|right|frame]]
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==Notebook==
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''Tell us more about your project. Give us background. Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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{| cellpadding="20" align="center"
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|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=06 }}
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|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=07 }}
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|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=08 }}
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|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=09 }}
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|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=10 }}
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|{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=11 }}
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|}
 +
===IGEM Part #s and Uses===
 +
 
 +
BBa_T9002 – Producer Controler Device–Plate2 Well 9A
 +
 
 +
BBa_I732017 – LacZ with our GFP 1—Plate 2 Well 3K
 +
 
 +
BBa_F2620– LuxR with terminator– Plate 2 Well 6E
 +
 
 +
BBa_I13522 –Tet Prop with GFP—Plate 2 Well 8A
 +
 
 +
BBa_T13521 Red Flouro tet RFP—Plate 2 Well 6O
 +
 
 +
BBa_pSB1C3—Plasmid—Plate 1 Well 3A
 +
 
 +
BBa_pSB1T3—Tetracycline resistant plasmid—Plate 1 Well 7A
 +
 
 +
BBa_pSB1AT3--high copy number plasmid carrying ampicillin and tetracycline resistance--Plate 1 Well 13A
 +
 
 +
== 6/28/10 ==
 +
Preparing SOB
 +
 
 +
== 6/30/10 ==
 +
 
 +
Richard Lab:Electroporation of E. coli
 +
 
 +
== 7/28/10 ==
 +
 
 +
Usage and extraction
 +
 
 +
== 8/24/10 ==
 +
 
 +
Today we will be pouring new plates for streaking new transformed cells.
 +
 
 +
== 8/27/10 ==
 +
 
 +
 
 +
We will be running a gel to determine if our DNA sample was successfully electroplated.
 +
 
 +
== 8/27/10 ==
 +
 
 +
Today we will be electroplating part KBBa_3131010
 +
 
 +
== 8/31/10 ==
 +
 
 +
Results of transformation - the plates had growth but (no) distinct colony pattern.
 +
 
 +
== 8/31/10 ==
 +
 
 +
After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH
 +
 
 +
== 9/1/10 ==
 +
 
 +
Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock.
 +
 
 +
== 9/2/10 ==
 +
 
 +
Results from streaking –
 +
 
 +
== 9/2/10 ==
 +
 
 +
Protocol For Making LB-Broth/Agar
 +
 
 +
== 9/3/10 ==
 +
 
 +
Today I’m finishing making the LB/Agar from yesterday.
 +
 
 +
== 9/9/10 ==
 +
 
 +
Due to conflict we will be changing from E Coli to yeast.
 +
 
 +
== 9/10/10 ==
 +
 
 +
Lux Casette Right Promoter BBa_I1051
 +
 
 +
== 9/14/10 ==
 +
 
 +
Today we found growth from our Electroporated E – Coli parts
 +
 
 +
== 9/15/10 ==
 +
 
 +
Today we will be extracting DNA from out electroporated cells/T9002
 +
B-galactosidase Assay
 +
 
 +
== 9/16/10 ==
 +
 
 +
Today we will wash
 +
 
 +
== 9/17/10 ==
 +
 
 +
Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work!
 +
 
 +
 
 +
== 10/18/10 ==
 +
 
 +
cutting enzymes
 +
 
 +
== 9/21/10 ==
 +
 
 +
Today we will electroporate Agrobacterium with part T9002
 +
 
 +
Electrophoresis
 +
 
 +
== 9/22/10 ==
 +
 
 +
Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample
 +
 
 +
== 9/22/10 ==
 +
 
 +
The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens
 +
 
 +
== 9/23/10 ==
 +
 
 +
Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces.
 +
 
 +
==9/24/10 ==
 +
 
 +
Today I’m testing absorbance of our trials @ 395nm
 +
 
 +
== 9/28/10 ==
 +
 
 +
Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10
 +
 
 +
== 9/30/10==
 +
LacZ without GFP also with RBS and terminator
 +
 
 +
 
 +
==10/1/10==
 +
 
 +
electrophoresis
 +
 
 +
==10/5/10==
 +
 
 +
pTet GFP
 +
 
 +
==10/7/10==
 +
 
 +
Electroporating 2 parts with E-coli bacteria
 +
 
 +
==10/8/10==
 +
 
 +
 
 +
LB-Lennox Broth & Electroporation
 +
 
 +
==10/12/10==
 +
hold until Friday
 +
 
 +
==10/13/10==
 +
Discoveries!
 +
 
 +
==10/15/10==
 +
 
 +
Using openwetware.org protocol
 +
 
 +
B-galactosidase Assay
 +
 
 +
BBL Trypticase soy Broth and Agar
 +
 
 +
DPBS
 +
 
 +
== 10/19/10==
 +
Protocol-- Electroporation man for E-Coli
 +
== 10/21/10 ==
 +
High Efficiency Electrotransformation of E.coli
 +
 
 +
== 10/25/10 ==
 +
 
 +
DNA from BlueGuy
 +
 
 +
== 10/26/10 ==
 +
Final steps pack and ship
 +
 
 +
 
|[[Image:IvyTech-South_Bend_team.png|right|frame|Your team picture]]
|[[Image:IvyTech-South_Bend_team.png|right|frame|Your team picture]]
|-
|-
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|align="center"|[[Image:IvyTech-South_Bend_logo.png|200px|right|frame]]
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|
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|align="center"|[[Team:IvyTech-South_Bend | Team Example]]
 
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<!--- The Mission, Experiments --->
<!--- The Mission, Experiments --->
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{| style="color:#1b2c8a;background-color:#006644;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
!align="center"|[[Team:IvyTech-South_Bend|Home]]
!align="center"|[[Team:IvyTech-South_Bend|Home]]
!align="center"|[[Team:IvyTech-South_Bend/Team|Team]]
!align="center"|[[Team:IvyTech-South_Bend/Team|Team]]
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!align="center"|[[Team:IvyTech-South_Bend/Safety|Safety]]
!align="center"|[[Team:IvyTech-South_Bend/Safety|Safety]]
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==Notebook==
 
-
 
-
You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
 

Latest revision as of 00:44, 28 October 2010

Discussion

Contents

Notebook

June
MTWTFSS
  1 2 3 4 5 6
7 8 9 10 11 12 13
14 15 16 17 18 19 20
21 22 23 24 25 26 27
28 29 30
July
MTWTFSS
      1 2 3 4
5 6 7 8 9 10 11
12 13 14 15 16 17 18
19 20 21 22 23 24 25
26 27 28 29 30 31
August
MTWTFSS
            1
2 3 4 5 6 7 8
9 10 11 12 13 14 15
16 17 18 19 20 21 22
23 24 25 26 27 28 29
30 31
September
MTWTFSS
    1 2 3 4 5
6 7 8 9 10 11 12
13 14 15 16 17 18 19
20 21 22 23 24 25 26
27 28 29 30
October
MTWTFSS
        1 2 3
4 5 6 7 8 9 10
11 12 13 14 15 16 17
18 19 20 21 22 23 24
25 26 27 28 29 30 31
November
MTWTFSS
1 2 3 4 5 6 7
8 9 10 11 12 13 14
15 16 17 18 19 20 21
22 23 24 25 26 27 28
29 30

IGEM Part #s and Uses

BBa_T9002 – Producer Controler Device–Plate2 Well 9A

BBa_I732017 – LacZ with our GFP 1—Plate 2 Well 3K

BBa_F2620– LuxR with terminator– Plate 2 Well 6E

BBa_I13522 –Tet Prop with GFP—Plate 2 Well 8A

BBa_T13521 Red Flouro tet RFP—Plate 2 Well 6O

BBa_pSB1C3—Plasmid—Plate 1 Well 3A

BBa_pSB1T3—Tetracycline resistant plasmid—Plate 1 Well 7A

BBa_pSB1AT3--high copy number plasmid carrying ampicillin and tetracycline resistance--Plate 1 Well 13A

6/28/10

Preparing SOB

6/30/10

Richard Lab:Electroporation of E. coli

7/28/10

Usage and extraction

8/24/10

Today we will be pouring new plates for streaking new transformed cells.

8/27/10

We will be running a gel to determine if our DNA sample was successfully electroplated.

8/27/10

Today we will be electroplating part KBBa_3131010

8/31/10

Results of transformation - the plates had growth but (no) distinct colony pattern.

8/31/10

After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH

9/1/10

Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock.

9/2/10

Results from streaking –

9/2/10

Protocol For Making LB-Broth/Agar

9/3/10

Today I’m finishing making the LB/Agar from yesterday.

9/9/10

Due to conflict we will be changing from E Coli to yeast.

9/10/10

Lux Casette Right Promoter BBa_I1051

9/14/10

Today we found growth from our Electroporated E – Coli parts

9/15/10

Today we will be extracting DNA from out electroporated cells/T9002 B-galactosidase Assay

9/16/10

Today we will wash

9/17/10

Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work!


10/18/10

cutting enzymes

9/21/10

Today we will electroporate Agrobacterium with part T9002

Electrophoresis

9/22/10

Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample

9/22/10

The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens

9/23/10

Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces.

9/24/10

Today I’m testing absorbance of our trials @ 395nm

9/28/10

Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10

9/30/10

LacZ without GFP also with RBS and terminator


10/1/10

electrophoresis

10/5/10

pTet GFP

10/7/10

Electroporating 2 parts with E-coli bacteria

10/8/10

LB-Lennox Broth & Electroporation

10/12/10

hold until Friday

10/13/10

Discoveries!

10/15/10

Using openwetware.org protocol

B-galactosidase Assay

BBL Trypticase soy Broth and Agar

DPBS

10/19/10

Protocol-- Electroporation man for E-Coli

10/21/10

High Efficiency Electrotransformation of E.coli

10/25/10

DNA from BlueGuy

10/26/10

Final steps pack and ship


Your team picture
IvyTech-South Bend logo.png


Home Team Official Team Profile Project Parts Submitted to the Registry Modeling Notebook Safety
Retrieved from "http://2010.igem.org/Team:IvyTech-South_Bend/Notebook"