Team:IvyTech-South Bend/Notebook
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<a href="https://2010.igem.org/Team:IvyTech-South_Bend"> <img src="https://static.igem.org/mediawiki/2010/f/f3/Home1.png" width=12%></a> | <a href="https://2010.igem.org/Team:IvyTech-South_Bend"> <img src="https://static.igem.org/mediawiki/2010/f/f3/Home1.png" width=12%></a> | ||
- | <a href="https://2010.igem.org/Team:IvyTech-South_Bend/Project"> <img src="https://static.igem.org/mediawiki/2010/ | + | <a href="https://2010.igem.org/Team:IvyTech-South_Bend/Project"> <img src="https://static.igem.org/mediawiki/2010/5/58/Image-_SBProject.PNG" width=11%></a> |
<width=80%><a href="https://2010.igem.org/Team:IvyTech-South_Bend/Parts"> <img src="https://static.igem.org/mediawiki/2010/7/79/Parts.png" width=15%></a> | <width=80%><a href="https://2010.igem.org/Team:IvyTech-South_Bend/Parts"> <img src="https://static.igem.org/mediawiki/2010/7/79/Parts.png" width=15%></a> | ||
- | <a href="https://2010.igem.org/Team:IvyTech-South_Bend/Modeling"> <img src="https://static.igem.org/mediawiki/2010/ | + | <a href="https://2010.igem.org/Team:IvyTech-South_Bend/Modeling"> <img src="https://static.igem.org/mediawiki/2010/0/0b/Image-SBModeling.PNG" width=11%></a> |
<a href="https://2010.igem.org/Team:IvyTech-South_Bend/Notebook"> <img src="https://static.igem.org/mediawiki/2010/9/95/Notebook.png" width=11%></a> | <a href="https://2010.igem.org/Team:IvyTech-South_Bend/Notebook"> <img src="https://static.igem.org/mediawiki/2010/9/95/Notebook.png" width=11%></a> | ||
<a href="https://2010.igem.org/Team:IvyTech-South_Bend/Safety"> <img src="https://static.igem.org/mediawiki/2010/8/84/Safety.png" width=11%></a> | <a href="https://2010.igem.org/Team:IvyTech-South_Bend/Safety"> <img src="https://static.igem.org/mediawiki/2010/8/84/Safety.png" width=11%></a> | ||
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- | + | <a href="https://2010.igem.org/Talk:Team:IvyTech-South_Bend/Notebook">Discussion</a> | |
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{|align="justify" | {|align="justify" | ||
- | |||
+ | {|align="justify" | ||
- | {{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=09 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=10 }}{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=11 }} | + | ==Notebook== |
+ | <html> | ||
+ | <style type="text/css"> | ||
+ | table.calendar { margin: 0; padding: 10px; } | ||
+ | table.calendar td { margin: 0; padding: 2px; vertical-align: top; } | ||
+ | table.month .heading td { padding:2px; background-color:#0f6666; color:#00ff00; text-align:center; font-size:120%; font-weight:bold; } | ||
+ | table.month .dow td { color:#00ff00; text-align:center; font-size:110%; } | ||
+ | table.month td.today { background-color:#ddd;} | ||
+ | table.month td { | ||
+ | border: none; | ||
+ | margin: 0; | ||
+ | padding: 1.5pt 2pt; | ||
+ | font-weight: bold; | ||
+ | font-size: 10pt; | ||
+ | text-align: center; | ||
+ | background-color: #000000; | ||
+ | } | ||
+ | #bodyContent table.month a { background:none; padding:0 } | ||
+ | .day-active { color:#00FF00 } | ||
+ | .day-empty { color:#006666 } | ||
+ | </style> | ||
+ | </html> | ||
+ | {| cellpadding="20" align="center" | ||
+ | |{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=06 }} | ||
+ | |{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=07 }} | ||
+ | |{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=08 }} | ||
+ | |{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=09 }} | ||
+ | |{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=10 }} | ||
+ | |{{ #calendar: title=Talk:Team:IvyTech-South_Bend |year=2010 | month=11 }} | ||
|} | |} | ||
- | + | ===IGEM Part #s and Uses=== | |
- | + | ||
- | + | BBa_T9002 – Producer Controler Device–Plate2 Well 9A | |
- | + | ||
- | + | BBa_I732017 – LacZ with our GFP 1—Plate 2 Well 3K | |
- | + | ||
+ | BBa_F2620– LuxR with terminator– Plate 2 Well 6E | ||
+ | |||
+ | BBa_I13522 –Tet Prop with GFP—Plate 2 Well 8A | ||
+ | |||
+ | BBa_T13521 Red Flouro tet RFP—Plate 2 Well 6O | ||
+ | |||
+ | BBa_pSB1C3—Plasmid—Plate 1 Well 3A | ||
+ | |||
+ | BBa_pSB1T3—Tetracycline resistant plasmid—Plate 1 Well 7A | ||
+ | |||
+ | BBa_pSB1AT3--high copy number plasmid carrying ampicillin and tetracycline resistance--Plate 1 Well 13A | ||
+ | |||
+ | == 6/28/10 == | ||
+ | Preparing SOB | ||
+ | |||
+ | == 6/30/10 == | ||
+ | |||
+ | Richard Lab:Electroporation of E. coli | ||
+ | |||
+ | == 7/28/10 == | ||
+ | |||
+ | Usage and extraction | ||
+ | |||
+ | == 8/24/10 == | ||
+ | |||
+ | Today we will be pouring new plates for streaking new transformed cells. | ||
+ | |||
+ | == 8/27/10 == | ||
+ | |||
+ | |||
+ | We will be running a gel to determine if our DNA sample was successfully electroplated. | ||
+ | |||
+ | == 8/27/10 == | ||
+ | |||
+ | Today we will be electroplating part KBBa_3131010 | ||
+ | |||
+ | == 8/31/10 == | ||
+ | |||
+ | Results of transformation - the plates had growth but (no) distinct colony pattern. | ||
+ | |||
+ | == 8/31/10 == | ||
+ | |||
+ | After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH | ||
+ | |||
+ | == 9/1/10 == | ||
+ | |||
+ | Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock. | ||
+ | |||
+ | == 9/2/10 == | ||
+ | |||
+ | Results from streaking – | ||
+ | |||
+ | == 9/2/10 == | ||
+ | |||
+ | Protocol For Making LB-Broth/Agar | ||
+ | |||
+ | == 9/3/10 == | ||
+ | |||
+ | Today I’m finishing making the LB/Agar from yesterday. | ||
+ | |||
+ | == 9/9/10 == | ||
+ | |||
+ | Due to conflict we will be changing from E Coli to yeast. | ||
+ | |||
+ | == 9/10/10 == | ||
+ | |||
+ | Lux Casette Right Promoter BBa_I1051 | ||
+ | |||
+ | == 9/14/10 == | ||
+ | |||
+ | Today we found growth from our Electroporated E – Coli parts | ||
+ | |||
+ | == 9/15/10 == | ||
+ | |||
+ | Today we will be extracting DNA from out electroporated cells/T9002 | ||
+ | B-galactosidase Assay | ||
+ | |||
+ | == 9/16/10 == | ||
+ | |||
+ | Today we will wash | ||
+ | |||
+ | == 9/17/10 == | ||
+ | |||
+ | Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work! | ||
+ | |||
+ | |||
+ | == 10/18/10 == | ||
+ | |||
+ | cutting enzymes | ||
+ | |||
+ | == 9/21/10 == | ||
+ | |||
+ | Today we will electroporate Agrobacterium with part T9002 | ||
+ | |||
+ | Electrophoresis | ||
+ | |||
+ | == 9/22/10 == | ||
+ | |||
+ | Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample | ||
+ | |||
+ | == 9/22/10 == | ||
+ | |||
+ | The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens | ||
+ | |||
+ | == 9/23/10 == | ||
+ | |||
+ | Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces. | ||
+ | |||
+ | ==9/24/10 == | ||
+ | |||
+ | Today I’m testing absorbance of our trials @ 395nm | ||
+ | |||
+ | == 9/28/10 == | ||
+ | |||
+ | Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10 | ||
+ | |||
+ | == 9/30/10== | ||
+ | LacZ without GFP also with RBS and terminator | ||
+ | |||
+ | |||
+ | ==10/1/10== | ||
+ | |||
+ | electrophoresis | ||
+ | |||
+ | ==10/5/10== | ||
+ | |||
+ | pTet GFP | ||
+ | |||
+ | ==10/7/10== | ||
+ | |||
+ | Electroporating 2 parts with E-coli bacteria | ||
+ | |||
+ | ==10/8/10== | ||
+ | |||
+ | |||
+ | LB-Lennox Broth & Electroporation | ||
+ | |||
+ | ==10/12/10== | ||
+ | hold until Friday | ||
+ | |||
+ | ==10/13/10== | ||
+ | Discoveries! | ||
+ | |||
+ | ==10/15/10== | ||
+ | |||
+ | Using openwetware.org protocol | ||
+ | |||
+ | B-galactosidase Assay | ||
+ | |||
+ | BBL Trypticase soy Broth and Agar | ||
+ | |||
+ | DPBS | ||
+ | |||
+ | == 10/19/10== | ||
+ | Protocol-- Electroporation man for E-Coli | ||
+ | == 10/21/10 == | ||
+ | High Efficiency Electrotransformation of E.coli | ||
+ | |||
+ | == 10/25/10 == | ||
+ | |||
+ | DNA from BlueGuy | ||
+ | |||
+ | == 10/26/10 == | ||
+ | Final steps pack and ship | ||
+ | |||
+ | |||
|[[Image:IvyTech-South_Bend_team.png|right|frame|Your team picture]] | |[[Image:IvyTech-South_Bend_team.png|right|frame|Your team picture]] | ||
|- | |- | ||
+ | |||
+ | |align="center"|[[Image:IvyTech-South_Bend_logo.png|200px|right|frame]] | ||
+ | |- | ||
+ | | | ||
+ | |||
+ | | | ||
| | | | ||
- | |||
|} | |} | ||
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!align="center"|[[Team:IvyTech-South_Bend/Safety|Safety]] | !align="center"|[[Team:IvyTech-South_Bend/Safety|Safety]] | ||
|} | |} | ||
- | |||
- | |||
- | |||
- | |||
- |
Latest revision as of 00:44, 28 October 2010
Discussion
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IGEM Part #s and Uses
BBa_T9002 – Producer Controler Device–Plate2 Well 9A
BBa_I732017 – LacZ with our GFP 1—Plate 2 Well 3K
BBa_F2620– LuxR with terminator– Plate 2 Well 6E
BBa_I13522 –Tet Prop with GFP—Plate 2 Well 8A
BBa_T13521 Red Flouro tet RFP—Plate 2 Well 6O
BBa_pSB1C3—Plasmid—Plate 1 Well 3A
BBa_pSB1T3—Tetracycline resistant plasmid—Plate 1 Well 7A
BBa_pSB1AT3--high copy number plasmid carrying ampicillin and tetracycline resistance--Plate 1 Well 13A
6/28/10
Preparing SOB
6/30/10
Richard Lab:Electroporation of E. coli
7/28/10
Usage and extraction
8/24/10
Today we will be pouring new plates for streaking new transformed cells.
8/27/10
We will be running a gel to determine if our DNA sample was successfully electroplated.
8/27/10
Today we will be electroplating part KBBa_3131010
8/31/10
Results of transformation - the plates had growth but (no) distinct colony pattern.
8/31/10
After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH
9/1/10
Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock.
9/2/10
Results from streaking –
9/2/10
Protocol For Making LB-Broth/Agar
9/3/10
Today I’m finishing making the LB/Agar from yesterday.
9/9/10
Due to conflict we will be changing from E Coli to yeast.
9/10/10
Lux Casette Right Promoter BBa_I1051
9/14/10
Today we found growth from our Electroporated E – Coli parts
9/15/10
Today we will be extracting DNA from out electroporated cells/T9002 B-galactosidase Assay
9/16/10
Today we will wash
9/17/10
Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work!
10/18/10
cutting enzymes
9/21/10
Today we will electroporate Agrobacterium with part T9002
Electrophoresis
9/22/10
Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample
9/22/10
The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens
9/23/10
Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces.
9/24/10
Today I’m testing absorbance of our trials @ 395nm
9/28/10
Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10
9/30/10
LacZ without GFP also with RBS and terminator
10/1/10
electrophoresis
10/5/10
pTet GFP
10/7/10
Electroporating 2 parts with E-coli bacteria
10/8/10
LB-Lennox Broth & Electroporation
10/12/10
hold until Friday
10/13/10
Discoveries!
10/15/10
Using openwetware.org protocol
B-galactosidase Assay
BBL Trypticase soy Broth and Agar
DPBS
10/19/10
Protocol-- Electroporation man for E-Coli
10/21/10
High Efficiency Electrotransformation of E.coli
10/25/10
DNA from BlueGuy
10/26/10
Final steps pack and ship
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