Team:IvyTech-South Bend/Notebook
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Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work! | Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work! | ||
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+ | == 10/18/10 == | ||
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+ | cutting enzymes | ||
== 9/21/10 == | == 9/21/10 == | ||
- | Today we will electroporate Agrobacterium with part T9002 | + | Today we will electroporate Agrobacterium with part T9002 |
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+ | Electrophoresis | ||
== 9/22/10 == | == 9/22/10 == | ||
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== 9/28/10 == | == 9/28/10 == | ||
- | Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10 | + | Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10 |
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+ | == 9/30/10== | ||
+ | LacZ without GFP also with RBS and terminator | ||
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+ | |||
+ | ==10/1/10== | ||
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+ | electrophoresis | ||
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+ | ==10/5/10== | ||
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+ | pTet GFP | ||
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+ | ==10/7/10== | ||
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+ | Electroporating 2 parts with E-coli bacteria | ||
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+ | ==10/8/10== | ||
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+ | LB-Lennox Broth & Electroporation | ||
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+ | ==10/12/10== | ||
+ | hold until Friday | ||
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+ | ==10/13/10== | ||
+ | Discoveries! | ||
+ | |||
+ | ==10/15/10== | ||
+ | |||
+ | Using openwetware.org protocol | ||
+ | |||
+ | B-galactosidase Assay | ||
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+ | BBL Trypticase soy Broth and Agar | ||
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+ | DPBS | ||
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== 10/19/10== | == 10/19/10== | ||
Protocol-- Electroporation man for E-Coli | Protocol-- Electroporation man for E-Coli | ||
== 10/21/10 == | == 10/21/10 == | ||
High Efficiency Electrotransformation of E.coli | High Efficiency Electrotransformation of E.coli | ||
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+ | == 10/25/10 == | ||
+ | |||
+ | DNA from BlueGuy | ||
+ | |||
+ | == 10/26/10 == | ||
+ | Final steps pack and ship | ||
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|[[Image:IvyTech-South_Bend_team.png|right|frame|Your team picture]] | |[[Image:IvyTech-South_Bend_team.png|right|frame|Your team picture]] | ||
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- | |[[Image:IvyTech-South_Bend_logo.png|200px|right|frame]] | + | |align="center"|[[Image:IvyTech-South_Bend_logo.png|200px|right|frame]] |
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Latest revision as of 00:44, 28 October 2010
Discussion
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IGEM Part #s and Uses
BBa_T9002 – Producer Controler Device–Plate2 Well 9A
BBa_I732017 – LacZ with our GFP 1—Plate 2 Well 3K
BBa_F2620– LuxR with terminator– Plate 2 Well 6E
BBa_I13522 –Tet Prop with GFP—Plate 2 Well 8A
BBa_T13521 Red Flouro tet RFP—Plate 2 Well 6O
BBa_pSB1C3—Plasmid—Plate 1 Well 3A
BBa_pSB1T3—Tetracycline resistant plasmid—Plate 1 Well 7A
BBa_pSB1AT3--high copy number plasmid carrying ampicillin and tetracycline resistance--Plate 1 Well 13A
6/28/10
Preparing SOB
6/30/10
Richard Lab:Electroporation of E. coli
7/28/10
Usage and extraction
8/24/10
Today we will be pouring new plates for streaking new transformed cells.
8/27/10
We will be running a gel to determine if our DNA sample was successfully electroplated.
8/27/10
Today we will be electroplating part KBBa_3131010
8/31/10
Results of transformation - the plates had growth but (no) distinct colony pattern.
8/31/10
After agar is dissolved, and allowed to cool, I will add Amp in different doses to specific plates. JH
9/1/10
Today the team will be making up the same plates as I made yesterday. Professor T. wants new Amp stock made to document how much is used into the stock.
9/2/10
Results from streaking –
9/2/10
Protocol For Making LB-Broth/Agar
9/3/10
Today I’m finishing making the LB/Agar from yesterday.
9/9/10
Due to conflict we will be changing from E Coli to yeast.
9/10/10
Lux Casette Right Promoter BBa_I1051
9/14/10
Today we found growth from our Electroporated E – Coli parts
9/15/10
Today we will be extracting DNA from out electroporated cells/T9002 B-galactosidase Assay
9/16/10
Today we will wash
9/17/10
Today we will be doing a DNA Extraction from/of part T9002 for electroporation this will determine if we have a stable host for our beast this will also Work!
10/18/10
cutting enzymes
9/21/10
Today we will electroporate Agrobacterium with part T9002
Electrophoresis
9/22/10
Today we have growth from electroporation done yesterday we are going to test by hitting with (AHL) the 20 mL grew not 5 mL sample
9/22/10
The only one that should glow should be (C)/(3) E – Coli w/transformed part in LB For Supernate of A.Tumafaciens
9/23/10
Today I have been setting up for the trial of our part T9002 inside agrobacteria to see if in the presence of AHL it floureces.
9/24/10
Today I’m testing absorbance of our trials @ 395nm
9/28/10
Today we will be picking 2 colonies from the Lacz, LuxR electroporated plates that were streaked on 9/22/10
9/30/10
LacZ without GFP also with RBS and terminator
10/1/10
electrophoresis
10/5/10
pTet GFP
10/7/10
Electroporating 2 parts with E-coli bacteria
10/8/10
LB-Lennox Broth & Electroporation
10/12/10
hold until Friday
10/13/10
Discoveries!
10/15/10
Using openwetware.org protocol
B-galactosidase Assay
BBL Trypticase soy Broth and Agar
DPBS
10/19/10
Protocol-- Electroporation man for E-Coli
10/21/10
High Efficiency Electrotransformation of E.coli
10/25/10
DNA from BlueGuy
10/26/10
Final steps pack and ship
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