Team:Freiburg Bioware/NoteBook/Labjournal/March

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1. Labday 31.03.2010

Sequence analysis

Investigators: Christian W., Bea, Kira, Achim, Kerstin, Hanna, Christian L. (Tobias, Kristian)

Theoretical cloning

Sequence analysis (Alignement) of Stratagene Vector with

AAV2
AAV5
AAV7
AAV8

Result:

Media:AAV_alignment.pdf
Notably, the alignment showed differences in the various AAV2 sequences, some of which are leading to frameshifts. The sequences date from 1983 and might not be completely accurate. We therefore rely on the Stratagene sequence from now on.

To do:

download and compare further AAV serotypes. (in Geneious)
compare literature
find out possible ways to modify the capsid

=> Go to Labjournal April (labday 2 - 5)

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 <li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/September">September part 1 (labday 107 - 123)</a></li>
 <li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/September2">September part 2 (labday 124 - 135)</a></li>
-<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October">October part 1 (labday 136 - 145 )</a></li>
+<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October">October part 1 (labday 136 - 149 )</a></li>
-<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October2">October part 2 (labday 146 - 155 )</a></li>
+<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October2">October part 2 (labday 150 - 166 )</a></li>
-<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October3">October part 3 (labday 156 - 166 )</a></li>
 <li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/November">November  (labday 167 - 170 )</a></li>
-<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/Cellculture">Cellculture </a></li>
+<li><a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/Cellculture">Cellculture</a></li>
 </ul>
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 ===<p style="font-size:17px; background-color:#00dd77;">1. Labday 31.03.2010</p>===
@@ Line 36: / Line 36: @@
 '''Theoretical cloning'''
 Sequence analysis (Alignement) of Stratagene Vector with
@@ Line 44: / Line 43: @@
 *AAV8
-'''result:'''
+'''Result:'''
 [[Media:AAV_alignment.pdf]]
 <br>
@@ Line 50: / Line 50: @@
 '''To do:'''
-*download and compare further AAV serotypes. (In Geneious)
+*download and compare further AAV serotypes. (in Geneious)
 *compare literature
-*find out possible ways to modify the Capsid
+*find out possible ways to modify the capsid
-<center>[https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/April '''=> Go to Labjournal April (labday 2 - 5)''']</center>
+<center>[https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/April '''=> Go to Labjournal April (labday 2 - 5)''']</center><br><br><br><br>
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