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Team:Freiburg Bioware/NoteBook/Labjournal
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| - | https://2010.igem.org/Team:Freiburg_Bioware/NoteBook => | + | <div style="height: 850px; width: 964px; margin: 15px 15px 15px 15px;"> |
| + | <div class="box_labjournal_195"> | ||
| + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/March"> | ||
| + | <h2 style="margin-left: 5px;">March</h2> | ||
| + | <p class="boxes_labjournal_day">Labday 1</p> | ||
| + | <img src="https://static.igem.org/mediawiki/2010/b/b4/Freiburg_10_labjournal_march_v1.JPG" class="image_labjournal" title="Our project starts with first sequence analysis."/> | ||
| + | <p class="boxes_labjournal_text">...watching gene sequences...</p> | ||
| + | </a> | ||
| + | </div> | ||
| - | < | + | <div class="box_labjournal_195"> |
| + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/April"> | ||
| + | <h2 style="margin-left: 5px;">April</h2> | ||
| + | <p class="boxes_labjournal_day">Labday 2- 5</p> | ||
| + | <img src="https://static.igem.org/mediawiki/2010/f/fc/Freiburg_10_labjournal_april_v1.JPG" class="image_labjournal" title="Scanning for iGEM restriction sites: | ||
| + | Vector plasmid; Thymidine kinase ;Cytosindeaminase" /> | ||
| + | <p class="boxes_labjournal_text">...ordering AAV-2 Helper-free System...</p> | ||
| + | </a> | ||
| + | </div> | ||
| - | < | + | <div class="box_labjournal_195"> |
| + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/May"> | ||
| + | <h2 style="margin-left: 5px;">May</h2> | ||
| + | <p class="boxes_labjournal_day">Labday 6- 17</p> | ||
| + | <img src="https://static.igem.org/mediawiki/2010/0/0f/Freiburg_10_labjournal_may_v1.JPG" class="image_labjournal" title="We took a closer look to the theoretical DNA- and amino acid sequence of the ITRs, questioned the role of the β-globin intron, had a closer look to the CMV promoter and performed our first cloning attempt." /> | ||
| + | <p class="boxes_labjournal_text">...theoretical cloning...</p> | ||
| + | </a> | ||
| + | </div> | ||
| - | < | + | <div class="box_labjournal_195"> |
| + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/June"> | ||
| + | <h2 style="margin-left: 5px;">June</h2> | ||
| + | <p class="boxes_labjournal_day">Labday 18- 45</p> | ||
| + | <img src="https://static.igem.org/mediawiki/2010/3/37/Freiburg_10_labjournal_june_v1.jpg" class="image_labjournal" title=" | ||
| + | We conducted our first site-directed mutagenesis. First cell culture steps: Splitting and seeding of AAV 293 and HT1080 cells; Calcium phosphate transfection; Transduction with virus particles containing YFP encoding sequence; Microscopy: Fluorescent transduced HT1080 cells. We planned to determine infectious virus titer via quantitative real-time PCR. For this purpose primers were designed, transduced HT1080 cells were harvested and prepared. Theoretical studies of the AAV structure: Impressions" /> | ||
| + | <p class="boxes_labjournal_text">...first transduced cells...</p> | ||
| + | </a> | ||
| + | </div> | ||
| - | < | + | <div class="box_labjournal_195"> |
| + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/July"> | ||
| + | <h2 style="margin-left: 5px;">July</h2> | ||
| + | <p class="boxes_labjournal_day">Labday 46- 75</p> | ||
| + | <img src="https://static.igem.org/mediawiki/2010/8/8d/Freiburg_10_labjournal_july_v1.JPG" class="image_labjournal" title="We developed a plan for modifying the surface exposed loops of the virus capsid. | ||
| + | For this purpose not only 5 iGEM restriction sites, but also the so called ViralBrick restriction sites located in the Rep/Cap sequence needed to be deleted: BamHI, SalI. BioBrick production of the inverted terminal repeats (ITRs) turned out to be difficult. Therefore the alternative “ITR fancy method” was developed. We successfully produced AAV2 left and right ITR fused to the RFC10 prefix. Further on we received first quantitative transduction results via flow cytometry. mGMK_TK30 was successfully cloned into the vector plasmid." /> | ||
| + | <p class="boxes_labjournal_text">...organizing the lab...</p> | ||
| + | </a> | ||
| + | </div> | ||
| - | < | + | <div class="box_labjournal_195"> |
| - | + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/August"> | |
| - | < | + | <h2 style="margin-left: 5px;">August I</h2> |
| - | < | + | <p class="boxes_labjournal_day">Labday 76- 92</p> |
| - | < | + | <img src="https://static.igem.org/mediawiki/2010/8/8b/Freiburg_10_labjournal_august1_v1.JPG" class="image_labjournal" /> |
| - | < | + | <p class="boxes_labjournal_text">...cloning parts...</p> |
| - | < | + | </a> |
| - | < | + | </div> |
| - | < | + | |
| - | < | + | <div class="box_labjournal_195"> |
| - | < | + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/August2"> |
| - | < | + | <h2 style="margin-left: 5px;">August II</h2> |
| - | < | + | <p class="boxes_labjournal_day">Labday 93- 106</p> |
| - | < | + | <img src="https://static.igem.org/mediawiki/2010/e/ec/Freiburg_10_labjournal_august2_v1.png" class="image_labjournal" /> |
| - | </ | + | <p class="boxes_labjournal_text">...assembled vector plasmid is working...</p> |
| + | </a> | ||
| + | </div> | ||
| + | |||
| + | <div class="box_labjournal_195"> | ||
| + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/September"> | ||
| + | <h2 style="margin-left: 5px;">September I</h2> | ||
| + | <p class="boxes_labjournal_day">Labday 107- 123</p> | ||
| + | <img src="https://static.igem.org/mediawiki/2010/2/25/Freiburg_10_labjournal_september1_v1.JPG" class="image_labjournal" /> | ||
| + | <p class="boxes_labjournal_text">...simply producing BioBricks...</p> | ||
| + | </a> | ||
| + | </div> | ||
| + | |||
| + | <div class="box_labjournal_195"> | ||
| + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/September2"> | ||
| + | <h2 style="margin-left: 5px;">September II</h2> | ||
| + | <p class="boxes_labjournal_day">Labday 124- 135</p> | ||
| + | <img src="https://static.igem.org/mediawiki/2010/1/18/Freiburg_10_labjournal_october2_v1.JPG" class="image_labjournal" /> | ||
| + | <p class="boxes_labjournal_text">...21x mutated RepCap is working...</p> | ||
| + | </a> | ||
| + | </div> | ||
| + | |||
| + | <div class="box_labjournal_195"> | ||
| + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October"> | ||
| + | <h2 style="margin-left: 5px;">October I</h2> | ||
| + | <p class="boxes_labjournal_day">Labday 136- 149</p> | ||
| + | <img src="https://static.igem.org/mediawiki/2010/e/e0/Freiburg_10_labjournal_october_v1.JPG" class="image_labjournal" /> | ||
| + | <p class="boxes_labjournal_text">...testing BioBricks by virus production...</p> | ||
| + | </a> | ||
| + | </div> | ||
| + | |||
| + | <div class="box_labjournal_195"> | ||
| + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/October2"> | ||
| + | <h2 style="margin-left: 5px;">October II</h2> | ||
| + | <p class="boxes_labjournal_day">Labday 150- 166</p> | ||
| + | <img src="https://static.igem.org/mediawiki/2010/2/22/Freiburg10_Oktober.png" class="image_labjournal" /> | ||
| + | <p class="boxes_labjournal_text">...Capsid modification works...</p> | ||
| + | </a> | ||
| + | </div> | ||
| + | |||
| + | <div class="box_labjournal_195"> | ||
| + | <a href="https://2010.igem.org/Team:Freiburg_Bioware/NoteBook/Labjournal/November"> | ||
| + | <h2 style="margin-left: 5px;">November</h2> | ||
| + | <p class="boxes_labjournal_day">Labday 166- 170</p> | ||
| + | <img src="https://static.igem.org/mediawiki/2010/9/93/Freiburg10_November.png" class="image_labjournal" /> | ||
| + | <p class="boxes_labjournal_text">...Jamboree!!!...</p> | ||
| + | </a> | ||
| + | </div> | ||
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Latest revision as of 03:17, 28 October 2010
