Team:Caltech/Week 3

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==Tuesday 6/29==
==Tuesday 6/29==
* Transformation results: Transformation of the ampicillin-resistant BioBricks seemed to be successful.  However, no bacterial growth was present in the plates and liquid cultures with tetracyclin present.  This seems to indicate a problem with the tetracyclin used.
* Transformation results: Transformation of the ampicillin-resistant BioBricks seemed to be successful.  However, no bacterial growth was present in the plates and liquid cultures with tetracyclin present.  This seems to indicate a problem with the tetracyclin used.
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** Of the plates with bacterial growth, there was evidence of dye production, indicating a successful transformation.
+
** Of the plates with bacterial growth ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 K274100 (red)], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 K274200 (orange)]), there was evidence of dye production, indicating a successful transformation.
 +
* Transformed 7 more bricks (pigments & our AND gate components):
 +
**
==Wednesday 6/30==
==Wednesday 6/30==

Revision as of 23:44, 29 June 2010


iGEM 2010



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Contents

Monday 6/28

  • Tested the success of the construct we made using digest and gel electrophoresis.
    • Digested ligation product from 6/26 with EcoRI and PstI.
    • Ran digests on gel along with 1kb and 100kb molecular ladders.
  • Gel result: No bands were visible other than those of the ladders. Amount of DNA used was most likely too low and thus the ligation product could not be confirmed.
Image of a test gel of our third ligation product. Note that the middle two lanes are sadly empty.
  • Transformed additional BioBricks from 2010 Spring DNA Distribution into DH5alpha cells
    • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 K274100], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 K274200], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274002 K274002], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274003 K274003], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274004 K274004]

Tuesday 6/29

  • Transformation results: Transformation of the ampicillin-resistant BioBricks seemed to be successful. However, no bacterial growth was present in the plates and liquid cultures with tetracyclin present. This seems to indicate a problem with the tetracyclin used.
    • Of the plates with bacterial growth ([http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 K274100 (red)], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 K274200 (orange)]), there was evidence of dye production, indicating a successful transformation.
  • Transformed 7 more bricks (pigments & our AND gate components):

Wednesday 6/30

Thursday 7/1

Friday 7/2

Weekend 7/3-4

 
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