Team:Caltech/Week 3

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** Ran digests on gel along with 1kb and 100kb molecular ladders.
** Ran digests on gel along with 1kb and 100kb molecular ladders.
* Gel result: No bands were visible other than those of the ladders. Amount of DNA used was most likely too low and thus the ligation product could not be confirmed.
* Gel result: No bands were visible other than those of the ladders. Amount of DNA used was most likely too low and thus the ligation product could not be confirmed.
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[[Image:CIT6-28gel.jpg|thumb]]
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[[Image:CIT6-28gel.jpg|thumb|Alt=An image of the test gel of our ligation product. The middle two lanes should have bands, but apparently have none. Lane 1: 100bp ladder, Lane 2: 3uL raw ligation product, Lane 3: 15uL ligation digest, Lane 4: 1kb ladder.|Image of a test gel of our third ligation product. Note that the middle two lanes are sadly empty.]]
* Transformed additional BioBricks from 2010 Spring DNA Distribution into DH5alpha cells
* Transformed additional BioBricks from 2010 Spring DNA Distribution into DH5alpha cells
** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 K274100], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 K274200], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274002 K274002], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274003 K274003], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274004 K274004]
** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 K274100], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 K274200], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274002 K274002], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274003 K274003], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274004 K274004]

Revision as of 01:24, 29 June 2010


iGEM 2010



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Contents

Monday 6/28

  • Tested the success of the construct we made using digest and gel electrophoresis.
    • Digested ligation product from 6/26 with EcoRI and PstI.
    • Ran digests on gel along with 1kb and 100kb molecular ladders.
  • Gel result: No bands were visible other than those of the ladders. Amount of DNA used was most likely too low and thus the ligation product could not be confirmed.
Image of a test gel of our third ligation product. Note that the middle two lanes are sadly empty.
  • Transformed additional BioBricks from 2010 Spring DNA Distribution into DH5alpha cells
    • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 K274100], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 K274200], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274002 K274002], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274003 K274003], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274004 K274004]

Tuesday 6/29

Wednesday 6/30

Thursday 7/1

Friday 7/2

Weekend 7/3-4

 
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