Team:Caltech/Week 3

From 2010.igem.org

(Difference between revisions)
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** Ran digests on gel along with 1kb and 100kb molecular ladders.
** Ran digests on gel along with 1kb and 100kb molecular ladders.
* Gel result: No bands were visible other than those of the ladders. Amount of DNA used was most likely too low and thus the ligation product could not be confirmed.
* Gel result: No bands were visible other than those of the ladders. Amount of DNA used was most likely too low and thus the ligation product could not be confirmed.
-
[[Image:CIT6-28.jpg]]
+
[[Image:CIT6-28gel.jpg|thumb]]
* Transformed additional BioBricks from 2010 Spring DNA Distribution into DH5alpha cells
* Transformed additional BioBricks from 2010 Spring DNA Distribution into DH5alpha cells
** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 K274100], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 K274200], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274002 K274002], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274003 K274003], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274004 K274004]
** [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 K274100], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 K274200], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274002 K274002], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274003 K274003], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274004 K274004]

Revision as of 01:17, 29 June 2010


iGEM 2010



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Contents

Monday 6/28

  • Tested the success of the construct we made using digest and gel electrophoresis.
    • Digested ligation product from 6/26 with EcoRI and PstI.
    • Ran digests on gel along with 1kb and 100kb molecular ladders.
  • Gel result: No bands were visible other than those of the ladders. Amount of DNA used was most likely too low and thus the ligation product could not be confirmed.
CIT6-28gel.jpg
  • Transformed additional BioBricks from 2010 Spring DNA Distribution into DH5alpha cells
    • [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274100 K274100], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274200 K274200], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274002 K274002], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274003 K274003], [http://partsregistry.org/wiki/index.php?title=Part:BBa_K274004 K274004]

Tuesday 6/29

Wednesday 6/30

Thursday 7/1

Friday 7/2

Weekend 7/3-4

 
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