Team:Caltech/Protocols

From 2010.igem.org

(Difference between revisions)
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* [[Team:Caltech/Competent Cells|Competent Cells]]
* [[Team:Caltech/Competent Cells|Competent Cells]]
* [[Team:Caltech/Electroporation|Transformation by Electroporation]]
* [[Team:Caltech/Electroporation|Transformation by Electroporation]]
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* Making stock solutions
 
* [[Team:Caltech/Glycerol Stocks|Freezer (glycerol) stocks]]
* [[Team:Caltech/Glycerol Stocks|Freezer (glycerol) stocks]]
* Plating antibiotics
* Plating antibiotics
* Sequencing primers
* Sequencing primers
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* Making synthase casette
 
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** Sources of sequences
 
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** Optimizing & checking genes
 
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* Chemical/Crosslinking reactions
 
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** Digesting substrate (hydrolysis) + epoxidation (MCPBA)
 
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** crosslinking (AIBN3) + UV initiation
 
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* Inducing lysis via 632nm light initiation
 
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* Making cell/glycerol/agar mixture
 
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** Applying this to plates
 
* Growing cells with PHA (and substrate oil)
* Growing cells with PHA (and substrate oil)
* [[Team:Caltech/Colony PCR|Colony PCR]]
* [[Team:Caltech/Colony PCR|Colony PCR]]
* [[Team:Caltech/Cloning|Two-day Cloning]]
* [[Team:Caltech/Cloning|Two-day Cloning]]
}}
}}

Revision as of 23:01, 24 October 2010


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