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Team:Caltech/Glycerol Stocks
From 2010.igem.org
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==Glycerol (Freezer) Stocks== | ==Glycerol (Freezer) Stocks== | ||
===Materials=== | ===Materials=== | ||
| - | * | + | * 0.5mL overnight LB culture |
| + | * 0.5mL 50% glycerol (sterile) | ||
| + | * Cryotube or 1.5mL Eppendorff | ||
| + | * Dry ice/ethanol or LN2 | ||
===Procedure=== | ===Procedure=== | ||
| - | # | + | # Mix overnight cell culture with equal parts 50% glycerol solution in cryotube. |
| + | # Label and add to dry ice/ethanol bath. | ||
| + | # Freeze for 10 minutes. Store at -80°C. | ||
}} | }} | ||
Latest revision as of 23:06, 24 October 2010
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 Glycerol (Freezer) StocksMaterials
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