Team:Caltech/Glycerol Stocks

From 2010.igem.org

(Difference between revisions)
 
Line 4: Line 4:
==Glycerol (Freezer) Stocks==
==Glycerol (Freezer) Stocks==
===Materials===
===Materials===
-
*  
+
* 0.5mL overnight LB culture
 +
* 0.5mL 50% glycerol (sterile)
 +
* Cryotube or 1.5mL Eppendorff
 +
* Dry ice/ethanol or LN2
===Procedure===
===Procedure===
-
#  
+
# Mix overnight cell culture with equal parts 50% glycerol solution in cryotube.
 +
# Label and add to dry ice/ethanol bath.
 +
# Freeze for 10 minutes. Store at -80°C.
}}
}}

Latest revision as of 23:06, 24 October 2010


iGEM 2010



Home

People

Project Details

Protocols

Completed Systems

Notebook

Biosafety

Human Impact

References

Support


Caltech logo watermark.png

Glycerol (Freezer) Stocks

Materials

  • 0.5mL overnight LB culture
  • 0.5mL 50% glycerol (sterile)
  • Cryotube or 1.5mL Eppendorff
  • Dry ice/ethanol or LN2

Procedure

  1. Mix overnight cell culture with equal parts 50% glycerol solution in cryotube.
  2. Label and add to dry ice/ethanol bath.
  3. Freeze for 10 minutes. Store at -80°C.
Error creating thumbnail: /websites/2010.igem.org/wiki/bin/ulimit4.sh: line 4: convert: command not found
Caltech footer.png


Locations of visitors to this page