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Team:British Columbia/Project DspB
From 2010.igem.org
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<h3><b>Introduction</b></h3> | <h3><b>Introduction</b></h3> | ||
| - | <p>Dispersin B (dspB) is an enzyme that degrades biofilms by catalyzing the hydrolysis of poly-ß-(1,6)-linked N-acetylglucosamine bond. These bonds exist as a polymer in the extracellular polymeric substance (EPS) as a polysaccharide adhesin; this adhesin is relevant in biofilm formation and integrity in both <i>Escherichia coli</i> and <i>Staphylococcus epidermidis</i>. According to Lu and Collins (reference paper), dspB effectively cleaves these bonds, thus affecting biofilm formation. Our goal is to isolate dspB from the host | + | <p>Dispersin B (dspB) is an enzyme that degrades biofilms by catalyzing the hydrolysis of poly-ß-(1,6)-linked N-acetylglucosamine bond. These bonds exist as a polymer in the extracellular polymeric substance (EPS) as a polysaccharide adhesin; this adhesin is relevant in biofilm formation and integrity in both <i>Escherichia coli</i> and <i>Staphylococcus epidermidis</i>. According to Lu and Collins (reference paper), dspB effectively cleaves these bonds, thus affecting biofilm formation. Our goal is to isolate dspB from the host <i>Actinobacillus actinomycetemcomitans</i></p> |
<br><h3><b>Approach</b></h3></br> | <br><h3><b>Approach</b></h3></br> | ||
<br><h3><b>Results</b></h3></br> | <br><h3><b>Results</b></h3></br> | ||
| - | + | <center><img src="https://2010.igem.org/Image:DspB_Assay_2.jpg" width=300px></src></center> | |
<br><h3><b>Discussion</b></h3></br> | <br><h3><b>Discussion</b></h3></br> | ||
Revision as of 04:20, 25 October 2010
Introduction
Dispersin B (dspB) is an enzyme that degrades biofilms by catalyzing the hydrolysis of poly-ß-(1,6)-linked N-acetylglucosamine bond. These bonds exist as a polymer in the extracellular polymeric substance (EPS) as a polysaccharide adhesin; this adhesin is relevant in biofilm formation and integrity in both Escherichia coli and Staphylococcus epidermidis. According to Lu and Collins (reference paper), dspB effectively cleaves these bonds, thus affecting biofilm formation. Our goal is to isolate dspB from the host Actinobacillus actinomycetemcomitans
Approach
Results
