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Team:BIOTEC Dresden/Protocols:Glycerol stock
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Latest revision as of 21:11, 25 October 2010
Materials
- 40% sterile Glycerol stock
- 1mL overnight culture
Procedure
- Chill electroporation cuvettes, DNA samples and tubes on ice.
- To an empty 2.0mL microfuge tube, add 1mL 40% Glycerol
- add 1mL of your overnight sample
- gently vortex to mix
- store in a freezer box at -80°C degrees
