Team:BIOTEC Dresden/Protocols:Glycerol stock



  • 40% sterile Glycerol stock
  • 1mL overnight culture


  • Chill electroporation cuvettes, DNA samples and tubes on ice.
  • To an empty 2.0mL microfuge tube, add 1mL 40% Glycerol
  • add 1mL of your overnight sample
  • gently vortex to mix
  • store in a freezer box at -80°C degrees
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