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Create the page "Kanamycin" on this wiki!

Team:Bielefeld-Germany/Project/Protocols
==Determination of minimal inhibitory concentration (MIC) of kanamycin== ...389212 (native ''virA'') in bacteria which are already containing K389101 (kanamycin resistance read out) using [[#Transformation via electroporation |electropo

30 KB (4,241 words) - 02:20, 28 October 2010
Team:Bielefeld-Germany/Project/Theory
...ts. Thus, we wanted to construct our system to lead in the expression of a kanamycin resistance after the induction of the VirA receptor, enabling the quick exc ... expression of aminoglycoside phosphotransferase (APH) that can inactivate kanamycin. The mode of inactivation is the transfer of the y-phosphate from ATP to th

35 KB (5,106 words) - 22:39, 27 October 2010
Team:Alberta/Notebook/BasePlasmids
Our original base plasmids contain a Kanamycin resistance cassette (p1003) bracketed by BsaI or BfuAI or BbsI cut sites. If cut with either BsaI or BfuAI or BbsI, the Kanamycin cassette is release with sticky

8 KB (1,183 words) - 01:09, 28 October 2010
Team:Alberta/Building Parts
...or BfuAI or BbsI cut sites. If cut with either BsaI or BfuAI or BbsI, the Kanamycin cassette is release with sticky ends characteristic of an A or a B BioByte. May 10, 2010 PCRed p1003 (Kanamycin cassette)with primers PrA_p1003+ and PrB'_p1003-

30 KB (4,641 words) - 19:55, 19 August 2010
Team:Harvard/vectors/parts
...d>pORE Open Series 1 (O1)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>pat</td><td><a href="http://openwetware.org/images/3/37/PORE_O1.gb" ...d>pORE Open Series 2 (O2)</td><td>Agrobacterium vector open series</td><td>Kanamycin</td><td>nptII</td><td><a href="http://openwetware.org/images/f/fc/PORE_O2.g

8 KB (1,499 words) - 23:19, 26 October 2010
Team:ULB-Brussels/Homologous
...sequences (fig.2). There are two resistance cassette plasmids, one with a kanamycin resistance marker (RCPKan) and the other with a chloramphenicol resistance <leg>Figure 2 : Resistance cassette kanamycin</leg>

19 KB (2,943 words) - 20:44, 27 October 2010
Team:Kyoto/Notebook1
... to LB plate (Amp+). And We didn't pre-culture B0015 despite its vector is Kanamycin-resistance. So, it was predicted that we will fail the transformation of pS |rowspan="2"|1||Kanamycin||○||rowspan="14"|At 37℃, 08/10 20:00-08/11 9:00

55 KB (7,098 words) - 02:17, 28 October 2010
Team:Bielefeld-Germany/Results/Characterization/K389011
...each plate were divided by the total number of colonies on a plate without kanamycin to calculate the survival rate φ. The ability of the bacterial populati ...ced population) indicate a common tendency. Under rising concentrations of kanamycin (x-axis) the survival rate of each population (y-axis) first decreases slow

4 KB (533 words) - 00:59, 28 October 2010
Team:LMU-Munich/Notebook/Apoptosis
- eGFP Biobrick: BBa_I714891 SDY_eGFP (Kanamycin) - 3 ml LB-Media + 4 µl Kanamycin

92 KB (13,163 words) - 18:04, 27 October 2010
Team:Tokyo Tech/Project/Artificial Cooperation System
...B (Fig3-0-3), production of LuxI is repressed by LasR/3OC12HSL complex and kanamycin resistance gene is activated by LasR/3OC12HSL complex. This means that 3OC1 ...ntration of 3OC12HSL and 3OC6HSL is too low to activate the expressions of kanamycin resistance gene and chloramphenicol resistance gene, respectively. 

13 KB (2,086 words) - 03:59, 28 October 2010
2010.igem.org/Team:Harvard/vectors/notebook
Ligated digested backbones and inserts. Transformed and plated on LB and Kanamycin. <ul><li>Prepared LB plates with 50 ug/ml kanamycin (15 ug of kanamycin per plate)</li>

20 KB (3,317 words) - 18:23, 22 July 2010
Team:Harvard/vectors/notebook
Ligated digested backbones and inserts. Transformed and plated on LB and Kanamycin. <ul><li>Prepared LB plates with 50 ug/ml kanamycin (15 ug of kanamycin per plate)</li>

20 KB (3,354 words) - 03:02, 25 October 2010
Team:Bielefeld-Germany/Results/Used
|style="border-style: solid; border-width: 1px"| Kanamycin resistance gene, <partinfo>K389005</partinfo>: <partinfo>K389005 SpecifiedC ...pectively. It was isolated from the BioBrick <partinfo>P1003</partinfo> (a kanamycin resistance gene with promoter and RBS).

31 KB (4,237 words) - 21:36, 27 October 2010
Team:INSA-Lyon/Project/Stage2/Results
<li>double transformation with ampicillin and kanamycin</li> <li>The lipid part with kanamycin</li>

4 KB (625 words) - 00:36, 28 October 2010
Page79
...ene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02). Plasmid 02 is fantastic base plasmid from which w PCR a Kanamycin resistance cassette fragment from p1003 with primers containing either the

24 KB (3,715 words) - 22:22, 8 July 2010
Team:Alberta/Notebook May
...ene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02). Plasmid 02 is fantastic base plasmid from which w PCR Kanamycin resistance cassette fragment from p1003 containing either the A/B' ends or

31 KB (4,965 words) - 18:29, 4 August 2010
Team:Stanford/Notebook/Lab Work/Week 2
*Transferred 50uL of transformed cells to agar plate with kanamycin. ====Making Kanamycin, Ampicillin Chloramphenicol Plates====

11 KB (1,622 words) - 02:05, 14 September 2010
Team:Bielefeld-Germany/Results/Submitted
===Neomycin / kanamycin resistance=== ...orm. We will use the BioBrick <partinfo>P1003</partinfo> as source for the kanamycin resistance gene.

9 KB (1,365 words) - 02:07, 28 October 2010
Team:Freiburg Bioware/NoteBook/Labjournal/September
Next steps: Picking clones of the trafo plates (containing Kanamycin) and perform the Mini-Prep. The obtaining constructs finally will be fused ...rmed according to standard protocol using BL21 cells and plates containing Kanamycin.

196 KB (29,094 words) - 21:34, 27 October 2010
Team:Michigan/Oil Sands August September
''' Transformation of Flu Operon + Kanamycin Backbone Ligation''' ...incubate longer as the slow growth may be due to the high concentration of Kanamycin (50mg/mL) added to the plates.

34 KB (5,403 words) - 01:36, 27 October 2010
Team:Tokyo Tech/Project/Artificial Cooperation System/modeling
...al Cooperation System. Fourth, in the contrast, when the system worked and Kanamycin existed, the number of cell B increased after temporal decline (Fig.3-4-4). ...ch_kan_sim_result.jpg|300px|left|thumb|Fig3-4-4 when the system worked and Kanamycin existed (work done by Shohei Kitano) ]]

9 KB (1,421 words) - 03:58, 28 October 2010
Team:Tsinghua/project/outline/m1
In donor plasmid A, we insert only one gene, kanamycin resistant gene (Kanr) to test our recombination. At the 5’ end of Kanr, w In donor plasmid B, we insert two genes, kanamycin resistant gene (Kanr) and Chloromycetin resistant gene (Chlr). At the 5’

20 KB (3,173 words) - 16:15, 27 October 2010
Team:Kyoto/Parts
...23-L||[http://partsregistry.org/Part:BBa_pSB1AK3 BBa_pSB1AK3]||Ampicillin, Kanamycin||129||3189 ...Ba_J23101]||2-17-F||[http://partsregistry.org/Part:BBa_pSB3K3 BBa_pSB3K3]||Kanamycin||919||2750

9 KB (1,303 words) - 22:47, 27 October 2010
Team:Michigan/Quorum Sensing
7/17 plates are no good -- they needed kanamycin *broth cultures had kanamycin added a day later -- too late to be cryostored, but they were transfered to

19 KB (2,960 words) - 01:14, 27 October 2010
Team:Kyoto/Protocols
#* Kanamycin: #*; Kanamycin: 0.5g

19 KB (3,027 words) - 02:42, 28 October 2010
Team:Alberta/Notebook July
...ene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02). Plasmid 02 is fantastic base plasmid from which w ...We picked as many colonies as possible and streaked on both LB plates with Kanamycin and Chloramphenicol resistance and LB plates with only Choramphenicol resis

30 KB (4,765 words) - 22:55, 10 August 2010
Team:Alberta/Notebook August
...ene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02). Plasmid 02 is fantastic base plasmid from which w ...We picked as many colonies as possible and streaked on both LB plates with Kanamycin and Chloramphenicol resistance and LB plates with only Choramphenicol resis

26 KB (3,927 words) - 21:02, 10 August 2010
Team:Michigan/Alex's Notebook
7/17 plates are no good -- they needed kanamycin *broth cultures had kanamycin added a day later -- too late to be cryostored, but they were transfered to

20 KB (3,132 words) - 04:36, 4 October 2010
Team:Michigan/Pili August September
2. Added 5 microliters of Kanamycin to each of the 50 mL tubes in step 1. *Marc adds 50uL of kanamycin onto each LB-agar plate and spread evenly with sterile glass beads

12 KB (1,774 words) - 03:05, 27 October 2010
Team:NCTU Formosa/Temperature Control
...n the appropriate media and antibiotic, such as agar plates with 25 µg/ml kanamycin. ...2% casamino acids, 1 mM MgSO4, 2 μM thiamine, 10 mM glucose, and 50 ng/ml Kanamycin antibiotic.) 

22 KB (2,885 words) - 02:18, 28 October 2010
Team:Calgary/3 August 2010
...ot grow of both regular Top10 cells and ccdB resistant cells on ampicillin-kanamycin and ampicillin-chloramphenicol plates.

3 KB (587 words) - 03:01, 27 October 2010
Team:Waterloo
...tor for both the measured part and the standard is pSB3K3 (low copy number kanamycin resistant BioBrick plasmid) with green ﬂuorescent protein gene as reporte I20260 on pSB3K3 inocluated into Kanamycin LB 

152 KB (22,732 words) - 17:38, 23 November 2010
Team:Caltech/Week 2
...e kanamycin test grew bacteria, suggesting that there is an issue with the kanamycin stock, which we will have to remake. ** Set up antibiotic test plates for kanamycin (kan) & tetracycline (tet), just as on [https://2010.igem.org/Team:Caltech/

8 KB (1,258 words) - 19:00, 21 July 2010
Team:TU Delft/6 July 2010 content
* rubB (Kanamycin) * ALDH (Kanamycin)

5 KB (699 words) - 19:23, 2 August 2010
Team:BCCS-Bristol/Wetlab/Notebook
...://partsregistry.org/Part:BBa_E0240 BBa_E0240] transformations, and agar + Kanamycin for [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] transformation. L ...g the [http://partsregistry.org/Part:BBa_J04450 BBa_J04450] biobrick are ''Kanamycin'' resistant, not Ampicillin resistant. Will reattempt overnight culture on

17 KB (2,636 words) - 19:06, 16 August 2010
Team:Alberta/Notebook June
...ene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02). Plasmid 02 is fantastic base plasmid from which w <ul>14μL either A/B' or B/A' Kanamycin resistance cassette (approx. 100ng/μL)</ul>

37 KB (5,929 words) - 18:34, 4 August 2010
Team:Harvard/vectors/vectors
...nfers plant resistance to <a href="http://en.wikipedia.org/wiki/Kanamycin">kanamycin</a>, to be used in transformant selection.</td> ...r, and pORE R3 the smgfp reporter. Both vectors confer plant resistance to kanamycin. </td>

4 KB (596 words) - 21:37, 24 October 2010
Team:Bielefeld-Germany/Project/Approach
..., a ''vir'' promoter and a readout or selection gene (luciferase, mRFP and kanamycin resistance, respectively): ...stems for the detection of the chosen substances. By varying the amount of kanamycin we will be able to carefully select some promising clones out of thousands

8 KB (1,195 words) - 03:10, 28 October 2010
Team:Calgary/Notebook/Safety And Protocols
...o;L of SOC (30 minutes for Ampicilin resistant plasmids and 60 minutes for kanamycin resistant plasmids) </li> <li> Add Antibiotic (10 µL Ampicillin, 5 µL Kanamycin, or 3 µL Chloramphenicol)</li>

27 KB (4,452 words) - 10:30, 27 October 2010
Team:Freiburg Bioware/testpage
* Kanamycin: 1 g Kanamycin was dissolved in 20 mL multipore-H2O, sterilized by filtration and filled i Further on two control plates containing ampicillin or kanamycin were prepared. Non-transformed cells were plated on them and also stored ov

20 KB (2,960 words) - 12:06, 6 September 2010
Team:Freiburg Bioware/NoteBook/Labjournal/May
* Kanamycin: 1 g Kanamycin was dissolved in 20 mL multipore-H2O, sterilized by filtration and filled i Further on two control plates containing ampicillin or kanamycin were prepared. Non-transformed cells were plated on them and also stored ov

20 KB (2,941 words) - 16:33, 5 January 2011
Team:Freiburg Bioware/NoteBook/Labjournal/August2
...o the standard protocol (XL1B). The cells were plated on a agar plate with Kanamycin. ...Cells: XL1b (60 µL), DNA amount per sample: 3 µL; cells were plated onto Kanamycin-plates.

160 KB (23,468 words) - 21:22, 27 October 2010
Team:Freiburg Bioware/NoteBook/Labjournal/September2
2 clones were picked of each ligation approach = 18 falcons (Kanamycin). 2 clones were picked of each ligation approach = 18 falcons (Kanamycin). 

135 KB (19,689 words) - 21:35, 27 October 2010
Team:Brown/Notebook/July21
===Test of kanamycin===

2 KB (324 words) - 23:02, 23 September 2010
Team:UC Davis/notebook/week11.html
...into are already kanamycin resistant. Thus, we need to replace I14015’s kanamycin resistant backbone.</li></ul> 

4 KB (659 words) - 05:50, 27 September 2010
Team:Yale/Our Project/Notebook/Week 7
...tocols/transformation">standard transformation procedure </a> and plate on Kanamycin plates. </li> ...ates, but also transformed DH5alpha cells with Q04121 and plated that with kanamycin. </li>

16 KB (2,556 words) - 02:53, 28 October 2010
Team:Harvard/fences/notebook
*made 4 LB+Kanamycin plates 15μL Kanamycin

74 KB (11,548 words) - 02:19, 27 October 2010
Team:Harvard/results
...nfers plant resistance to <a href="http://en.wikipedia.org/wiki/Kanamycin">kanamycin</a>, to be used in transformant selection.</td> ...r, and pORE R3 the smgfp reporter. Both vectors confer plant resistance to kanamycin. </td>

11 KB (1,766 words) - 18:08, 27 October 2010
Team:Paris Liliane Bettencourt/Project/Memo-cell/Results
...cated in pir+ strains. These plasmids also carried the Chloramphenicol and Kanamycin resistance cassettes. 1% and then replicated on a plate with Kanamycin. Colonies which have excised will

7 KB (1,032 words) - 03:53, 28 October 2010
Team:Calgary/1 August 2010
...ction digest and attempting to construct the CpxP promoter into Ampicillin-kanamycin and ampicillin-chloramphenicol plasmids. I also did this construction today ...of the CpxR promoter from an ampicillin resistant plasmid to an ampicillin-kanamycin resistant plasmid. Transformed the AK CpxR into competent cells and plated.

1 KB (177 words) - 04:54, 23 August 2010
Team:KIT-Kyoto/Parts
...info>pSB3k3</partinfo>(<partinfo>BBa_J04450</partinfo>)||Plasmid backbone||Kanamycin||738bp||2750bp||promoter(LacI) +RBS+mRFP+T+T ...info>pSB1AK3</partinfo>(<partinfo>BBa_I732950</partinfo>)||Protein domain||Kanamycin||3230bp||2079bp||RBS+LacZ+T+T

5 KB (718 words) - 03:16, 27 October 2010
Team:Nevada/Notebook
** Made Kanamycin resistant LB plates ** Screened 35S-GFP candidates on chloramphenicol and kanamycin

31 KB (4,781 words) - 21:20, 27 October 2010
Team:UNIPV-Pavia/Material Methods/Protocols
* Kanamycin 50 mg/ml (in water) * Kanamycin 20 ug/ml

21 KB (3,306 words) - 07:11, 26 May 2011
Team:Calgary/12 May 2010
...e, we also plan on making plates with dual-antibiotics like ampicillin and kanamycin combined or ampicillin and chloramphenicol. These antibiotics were chosen b

2 KB (271 words) - 02:48, 23 August 2010
Team:Calgary/3 June 2010
...e Registry plates) and plated all on ampicillin, kanamycin, and ampicillin-kanamycin plates.

597 B (85 words) - 02:51, 23 August 2010
Team:Calgary/8 June 2010
...and Chris's spread plates of Ampicillin - Chloramphenicol and Ampicillin - Kanamycin from the Parts Registry.]] ...g|350px|thumb|Dev, Jeremy, and Chris's spread plates of the Ampicillin and Kanamycin plasmid backbones from the Parts Registry.]]

3 KB (455 words) - 02:57, 23 August 2010
Team:Calgary/17 June 2010
... restarted the construction, transformed it into TOP10 cells and plated on kanamycin for oveneright growth (with Dev's help) and tomorrow I shall start some mor ...FP from the Registry as well as attempting a plasmid switch of J23032 to a kanamycin resistant plasmid.

3 KB (464 words) - 03:10, 23 August 2010
Team:Purdue/Notebook
We made a 50 µg/mL solution of kanamycin and LB agar broth which we then used to make 21 plates for further E. coli We added 5mL of agar soluntion and 25 micrograms of L-kanamycin to two tubes and then placed one cell colony in each of the tubes. We then

4 KB (674 words) - 15:38, 11 September 2010
Team:RMIT Australia/Notebook
Transformation of RFP in Ampicillin, Kanamycin and Chloroamphenicol backbones. They will act as spare backbones. ...d backbone. It was thought that it was pSB1A3, but in fact it was pSB1AK3. Kanamycin has a ClaI site.

28 KB (3,884 words) - 05:06, 14 October 2010
Team:Michigan/Media Recipes
'''Kanamycin Antibiotic Stock Solution (50 mg/mL)''' *250 mg of kanamycin

4 KB (680 words) - 23:52, 26 October 2010
Team:UNIPV-Pavia/Calendar/June/settimana2
...r plates added with Ampicillin, except for <partinfo>BBa_E2050</partinfo> (Kanamycin). ...promoters. Probably, this spurious transcription is due to the promoter of Kanamycin resistance, that is oriented in the same direction.

8 KB (1,221 words) - 12:12, 22 October 2010
Team:Calgary/6 July 2010
***-5uL Kanamycin Antibiotic ... plasmid switch that was done yesterday of B0034 from Kan to an ampicillin-kanamycin plasmid. There was no growth from the use of the enzymes EcoRI and PstI so

5 KB (895 words) - 03:35, 23 August 2010
Team:TU Munich/Lab
**200 µl plated on a Kanamycin-containing Plate **remaining 18 µl of samples 1, 3, 6, and 7 added to 5 ml LB + kanamycin

176 KB (25,809 words) - 12:04, 22 November 2010
Team:Davidson-MissouriW/Notebook
<li>Ligated “cre construct” into low, medium, and high copy kanamycin resistant vectors <li>Planned kanamycin experiments

14 KB (2,095 words) - 04:33, 26 October 2010
Team:Stanford/Notebook/Lab Work/Week 1
...LB plates (with the appropriate antibiotic added – usually Ampicillin or Kanamycin). Grow overnight. #plate100uL on appropriate plate (kanamycin this time), incubate 12-16 hours before picking colonies

6 KB (979 words) - 22:10, 5 August 2010
Team:Stanford/Notebook/Lab Work/Week 7
Made LB Agar plates (chloramphenicol, kanamycin) Made LB Agar plates (chloramphenicol, kanamycin)

11 KB (1,712 words) - 01:15, 27 October 2010
Team:TU Delft/20 July 2010 content
...g=19_July_2010 yesterday's] transformants onto kanamycin plates. Growth on kanamycin plates would indicate the presence of the pSB1AK2 plasmid and thus that the

8 KB (1,160 words) - 12:44, 13 August 2010
Team:Calgary/27 August 2010
...lin-chloramphenicol plasmids. They were plated on just chloramphenicol and kanamycin plates so as not to induce as much stress on the cells. As well, I helped H

2 KB (278 words) - 04:46, 31 August 2010
Team:Brown/Notebook/July13
...tively thinking that the LB/Agar that was prepared was contaminated with a kanamycin-resistant strain.

2 KB (343 words) - 19:56, 9 August 2010
Team:Nevada/BY-2 (NT1)Transformation Protocol
Add kanamycin (100 mg/ml) 13. Resuspend cells in approximately 5 ml NTC and plate 2.0 ml on 2 NTKC (kanamycin 100 ug/ml carbenicillin 500 ul/ml) plates. When there is no longer lots of

7 KB (1,105 words) - 19:36, 27 October 2010
Team:UCL London/Week 10
... 1xChlorophenical. This time, we successfully transformed the parts in the Kanamycin agar plate. These parts were essential as we planned on fermenting them the

3 KB (494 words) - 20:09, 26 October 2010
Team:sheffield/week8
...att were autoclaving, pouring agar plates and preparing LB. Ampicillin and kanamycin plates were prepared separately and together. The restriction digest perfor ...plated out some cells to see if the transformations were successful on the Kanamycin and Ampicillin plates. At 5pm Tom interviewed Virginia.

3 KB (448 words) - 16:16, 24 October 2010
Team:Tsinghua/Notebook/2 August 2010
We PCR amplified the Kanamycin resistance and Chloramphenicol resistance genes. We also amplified the repl ...petent trans5a cells with these T vectors and placed them on LB media with Kanamycin.

2 KB (204 words) - 17:10, 26 October 2010
Team:Edinburgh/Notebook/Blue light sensor
*K191003 - kanamycin *K191009 – kanamycin

18 KB (2,815 words) - 02:12, 28 October 2010
Team:Freiburg Bioware/NoteBook/Labjournal/August
... that this expression plasmid mustn't digested with NgoMIV and it contains Kanamycin-resistance! | EGFP_C1|| align="right" |Kanamycin || align="right" |

195 KB (27,796 words) - 21:36, 27 October 2010
Team:Groningen/Antibiotics Concentrationas and Organisms
[[Kanamycin]] 50 mg/ml Kanamycin (1000x) Stock

542 B (76 words) - 15:42, 25 October 2010
Team:Panama/8 September 2010
Part # BBa_B1002 Plate #1 welt 4B Plasmid psB1AK3 Amp resistance & kanamycin. 2 plates of kanamycin: control & terminator.

552 B (78 words) - 03:20, 28 October 2010
Team:Brown/Notebook/July17
...ight into 4 mL of fresh LB and 3.57µl kanamycin to make 5 mL culture with Kanamycin at 25 µg/mL and began incubating.

1 KB (219 words) - 19:20, 11 September 2010
Team:TU Delft/Protocols
...are ampicillin (A), chloramphenicol (C), erythromycin (E), gentamycin (G), kanamycin (K), neomycin (N), nalidixic acid (Na), rifampicin (R), spectinomycin (S), |Kanamycin

53 KB (8,186 words) - 22:17, 27 October 2010
Team:UCL London/Antibiotic Concentrations
| Kanamycin | Kanamycin

2 KB (196 words) - 20:48, 24 October 2010
Team:KIT-Kyoto/Parts1
|<partinfo>pSB3k3</partinfo>(<partinfo>BBa_J04450</partinfo>)||Kanamycin||738||2750||promoter(LacI) +RBS+mRFP+T+T |<partinfo>pSB1AK3</partinfo>(<partinfo>BBa_I732950</partinfo>)||Kanamycin||3230||2079||RBS+LacZ+T+T

2 KB (304 words) - 06:15, 16 September 2010
Team:British Columbia/Notebook
Kanamycin can last 2-3 months. I don't know about chloramphenicol and tetracycline (n Kanamycin can last 2-3 months. I don't know about chloramphenicol and tetracycline (n

24 KB (4,253 words) - 02:57, 28 October 2010
Team:Panama/9 September 2010
Kanamycin: 0 colonies. A colony of LB ampicillin plate & a colony of LB kanamycin plate were taken, & placed in liquid medium with antibiotics in agitation a

439 B (60 words) - 00:57, 28 October 2010
Team:MIT bexp
...as added 1:40 to 4mL melted M9 minimal top agar and kept at 50°C while Kanamycin and Ampicillin were added. If the composite biobrick was not K415022 or K41

4 KB (714 words) - 16:53, 27 October 2010
Team:Panama/10 September 2010
Ter K1 ---> Terminator grown in medium with kanamycin. Ter K2 ---> Terminator grown in medium with kanamycin.

2 KB (302 words) - 03:22, 28 October 2010
Team:Nevada/plasmid w terminator sequences
...nfers kanamycin resistance in bacteria carrying this plasmid. 50 μg/mL of kanamycin should be used to select for bacterial transformants containing this plasmi

5 KB (748 words) - 19:33, 27 October 2010
Team:BIOTEC Dresden/Materials
<td>Kanamycin</td> <td>LB Plates (100μg/ml Ampicillin, 15μg/ml Chloramphenicol, 30μg/ml Kanamycin, 10μg/ml Tetracyclin)</td>

4 KB (619 words) - 19:14, 25 October 2010
Team:Paris Liliane Bettencourt/Project/Population counter/Results
... plated on plates containing ampicillin and glucose. We didn't have to put kanamycin into the plates because we don't want recombinations to happen on the plate

11 KB (1,678 words) - 03:55, 28 October 2010
Team:Heidelberg/Notebook/Material
|Kanamycin||50 µg/ml||ddH2O |align="right"| pSB1K3 || 3A assembly cloning || Kanamycin Resistance || [http://partsregistry.org/Part:pSB1K3 Parts Registry] ||

9 KB (1,105 words) - 23:14, 27 October 2010
Team:Groningen/Antibiotics and Concentrationas
[[Kanamycin]] 50 mg/ml Kanamycin (1000x) Stock

542 B (76 words) - 15:44, 25 October 2010
Team:Tsinghua/experiments/results
Left：the size of every part (vector backbone, chloromycetin , and kanamycin): 1k，1.6k，1.1k ...s the figure. The left six lane are belong to a group of Negative Control. Kanamycin is about 700bp, while the recombination rate is 100%

5 KB (902 words) - 17:39, 27 October 2010
Team:Bielefeld-Germany/Results/Characterization/Exemplary results DM
...system]], colonies growing on the agar plates with 100 µg mL-1 kanamycin must either be induceable by one of the tested substances, or had received ... we extracted the 23 promising VirA variants and changed the read out from kanamycin resistance to the expression of quantifiable luciferase procedure was descr

5 KB (751 words) - 02:05, 28 October 2010
Team:Calgary/4 August 2010
...onstruction of CpxR in front of I13507 (RFP composite part). Plated on NEW kanamycin plates.

4 KB (731 words) - 04:55, 23 August 2010
Team:Calgary/10 August 2010
...ted cells. I also set up a colony PCR of 16 colonies of CpxP in Ampicillin-kanamycin and cut with either XbaI and SpeI. Overnight cultures were also made of the

4 KB (799 words) - 03:01, 27 October 2010
Team:Calgary/18 August 2010
...(for cloning purposes) and constructed I0500-I13507. Emily and I also made kanamycin-chloramphenicol and chloramphenicol plates.

2 KB (382 words) - 23:43, 26 October 2010
Team:EPF Lausanne/Project parts
*Kan = Resistance to Kanamycin

7 KB (963 words) - 20:33, 27 October 2010
Team:Calgary/14 May 2010
...es with ampicillin showed large quantities of growth while the plates with kanamycin had no growth whatsoever. We grew them on both plates because we were unsur

786 B (126 words) - 02:49, 23 August 2010
Team:TU Delft/8 June 2010 content
* pSB1K3 (Kanamycin)

4 KB (642 words) - 11:19, 24 August 2010
Team:Calgary/14 June 2010
...0430 (from colonies 3 and 4) to a pSB1K2 plasmid. Each was plated onto two kanamycin plates.

4 KB (736 words) - 03:05, 23 August 2010
Team:Calgary/22 June 2010
...of R0040 and original E0430 yesterday was on a pSB1A2 plasmid. I plated on kanamycin agar plates. Thus, no growth. (Clever.) I will retry the construction once

1 KB (193 words) - 03:16, 23 August 2010
Team:TU Delft/protocols/buffers stock solutions
|Kanamycin

2 KB (331 words) - 18:45, 12 September 2010
Reagents
Kanamycin - Sigma K4000-5G $59.60

1 KB (204 words) - 00:49, 19 October 2010
Team:Tokyo Tech
Fourth, in the contrast, when the system worked and Kanamycin existed, the number of cell B increased after temporal decline. From the ab

11 KB (1,712 words) - 22:34, 23 November 2010
Team:WashU/Parts
:*The ampicillin and kanamycin high replication plasmid. [http://partsregistry.org/Part:pSB1AT3 pSB1AT3]

970 B (137 words) - 06:09, 27 October 2010
Team:TU Delft/protocols/provided biobricks
...are ampicillin (A), chloramphenicol (C), erythromycin (E), gentamycin (G), kanamycin (K), neomycin (N), nalidixic acid (Na), rifampicin (R), spectinomycin (S),

1 KB (166 words) - 09:19, 12 September 2010
Team:Calgary/9 July 2010
*1 colony of E1010 in its original Kanamycin plasmid

3 KB (463 words) - 03:37, 23 August 2010
Team:Calgary/19 July 2010
Today, I redid the transformation of the CpxP promoter into both ampicillin-kanamycin and ampicillin-chloramphenicol plasmids with standard procedures. The diges

4 KB (694 words) - 04:09, 23 August 2010
Team:Calgary/20 July 2010
... colony PCR of the CpxP promoter that was transformed into both ampicillin-kanamycin and ampicillin-chloramphenicol plasmids. The results of the colony PCR were

4 KB (787 words) - 22:38, 26 August 2010
Team:Calgary/21 July 2010
Today, I did Plasmid preparations of the CpxP promoter in both ampicillin-kanamycin and ampicillin-chloramphenicol plasmids. The colony PCR from both of the Cp

3 KB (554 words) - 02:46, 27 October 2010
Team:Calgary/26 July 2010
...pxP promoters that were put into Ampicillin-chloramphenicol and ampicillin-kanamycin plasmids. The first gel that was run was of the promoters having been diges

4 KB (698 words) - 04:50, 23 August 2010
Team:Warsaw/Stage1/RBSMeas
...e spun down, resuspended in equal amount of PBS supplemented with 500mg/ml kanamycin and measured on BD FacsCalibur flow cytometer.</li>

3 KB (534 words) - 11:04, 26 October 2010
Team:TU Munich/Parts
| Basic plasmid backbone with kanamycin resistance

18 KB (2,646 words) - 19:37, 20 November 2010
Team:Purdue/E. coli transformation
#Added 1.5 μl luciferase plasmid DNA (with kanamycin resistance) to eppendorf tube

812 B (126 words) - 14:28, 13 July 2010
Team:EPF Lausanne/Notebook/week5
...t we obtained then didn't seem to be the wild type (it grew on plates with Kanamycin).

5 KB (774 words) - 19:47, 24 October 2010
Team:UNAM-Genomics Mexico/Notebook
2.Grow up the transformed cells in LB medium with kanamycin antibiotic.

30 KB (4,723 words) - 02:47, 24 November 2010
Team:EPF Lausanne/Notebook/week4
Today we transform competent Asaia with a GFP + kanamycin (resistance) following this [https://static.igem.org/mediawiki/2010/a/ac/EP

6 KB (880 words) - 19:47, 24 October 2010
Team:Valencia/Notebook/July 3week
... colony was cultured in a tube filled with 5 ml of LB medium and 5 µl of kanamycin and kept at 37ºC in an stove for the whole night. We will profit this cul

5 KB (920 words) - 16:47, 24 October 2010
Team:Stanford/NotebookPage/30 June 2010
#plate100uL on appropriate plate (kanamycin this time), incubate 12-16 hours before picking colonies

627 B (104 words) - 20:15, 19 July 2010
Team:Stanford/Notebook/Lab Work/Week 6
...-term and RFP-term- inoculate PCR reaction, then spread on small square of Kanamycin plate

18 KB (2,738 words) - 00:57, 27 October 2010
Team:Brown/Notebook/July8
*Spread 40 µl of 0.1 M IPTG over five Kanamycin plates and one Ampicillin plate.

3 KB (489 words) - 17:29, 21 July 2010
Team:Brown/Notebook/July9
*Kanamycin – 25-50 µg/mL

2 KB (248 words) - 17:33, 21 July 2010
Team:TU Delft/21 July 2010 content
The transformants that were replated on kanamycin plates produced colonies, indicating that hypothesis II was invalid. To tes

11 KB (1,498 words) - 21:07, 16 August 2010
BIOTEC Dresden/Notepad/19 July 2010
Both with kanamycin resistance.

456 B (58 words) - 21:27, 27 October 2010
Team:EPF Lausanne/Project asaia
...d the behaviour of Asaia to 7 other antibiotics like Tetracyclin (Tet) and Kanamycin (Kan) at various concentrations.

6 KB (862 words) - 20:13, 27 October 2010
Team:Edinburgh/Bacterial/Red light sensor
Thus, we retrieved transformants from the kanamycin-resistant version of <a href="http://partsregistry.org/Part:BBa_K098010">BB

19 KB (3,162 words) - 02:27, 28 October 2010
Team:BCCS-Bristol/Wetlab/BioBricks
Vector used: pSB1K3. Kanamycin resistance used as selective marker.

2 KB (337 words) - 09:32, 12 August 2010
Team:Davidson-MissouriW/Sponsors
...thank David Bikard from the 2007 Paris iGEM team for his help with growing kanamycin-resistant plasmids.

3 KB (362 words) - 18:51, 29 July 2010
Team:Bielefeld-Germany/Results
...ut screening system]] with a selective system based on the expression of a kanamycin resistance. We tried to modify the receptor by different modification strat

4 KB (532 words) - 02:19, 28 October 2010
Page80
...ene (the "death" gene) between our prefix and suffix removing the gene for Kanamycin resistance (plasmid 02). Plasmid 02 is fantastic base plasmid from which w

9 KB (1,272 words) - 20:17, 30 July 2010
Team:LMU-Munich/Notebook/Protocols/7 Measurement of competence
** Ampicillin and kanamycin appear to do fine with 1 hour growth

1 KB (202 words) - 13:21, 13 August 2010
Team:Bielefeld-Germany/Results/Characterization
...ar plates with chloramphenicol, ampicillin and different concentrations of kanamycin. Comparison between cells that were induced with acetosyringone with cells

14 KB (1,826 words) - 01:07, 28 October 2010
Team:Tsinghua/Notebook/4 August 2010
...ed competent E. coli cell, trans5a and placed the cells onto LB media with Kanamycin.

460 B (68 words) - 06:52, 25 October 2010
Team:Osaka/Protocols
... the culture onto LB with appropriate antibiotics, for example ampicillin, kanamycin,chloramphenicol, etc. Incubate at 37℃ overnight.

7 KB (1,091 words) - 00:52, 28 October 2010
Team:Brown/Notebook/July12
*Prepared IPTG plates with kanamycin

2 KB (351 words) - 19:49, 9 August 2010
Team:DTU-Denmark/Notebook
..., perhaps the cells were not left long enough to recover and hence express kanamycin resistance. Another reason could be the digestions made of pAT09-pAT11, as

88 KB (15,694 words) - 16:13, 27 October 2010
Team:Edinburgh/Project/Future
... Knowing this we have switched strain to K12. We are also now using kanamycin resistance instead of chloramphenicol resistance.

12 KB (2,007 words) - 02:20, 28 October 2010
Team:Sheffield/16 August 2010
...att were autoclaving, pouring agar plates and preparing LB. Ampicillin and kanamycin plates were prepared separately and together. The restriction digest perfor

585 B (92 words) - 10:34, 17 August 2010
Team:Sheffield/17 August 2010
...plated out some cells to see if the transformations were successful on the Kanamycin and Ampicillin plates. At 5pm Tom interviewed Virginia.

622 B (103 words) - 16:01, 18 August 2010
Team:Groningen/Growing Streptomyces
*Kanamycin (Km) 10 mg/ml (4 °C) 20 (μg/ml) (:500)

1,022 B (99 words) - 17:19, 9 September 2010
Team:UCL London/Week 5
Kanamycin backbone for assembly.

4 KB (637 words) - 20:09, 26 October 2010
Team:Cambridge/Notebook/9
...transformations for each construct just in case, we plated them all out on kanamycin plates to select for the DNA 2.0 in house vector [https://www.dna20.com/ind

3 KB (423 words) - 09:15, 9 September 2010
Team:UCL London/Our BioBricks
| Kanamycin Resistance Backbone

6 KB (825 words) - 22:42, 27 October 2010
Team:EPF Lausanne/Notebook/week7
We are still not sure if Asaia "WT" is resistant to kanamycin or not. Maybe we will have to use another resistance in our biobricks, TetR

4 KB (626 words) - 19:48, 24 October 2010
Team:Mexico-UNAM-CINVESTAV/Protocols
... on LB plates (with appropriate antibiotic added – usually Ampicillin or Kanamycin.) Grow overnight.

4 KB (647 words) - 07:59, 27 October 2010
Team:Edinburgh/Results
... to work with this protocol, possibly replacing the cat gene with a kanamycin resistance gene.

23 KB (3,730 words) - 03:59, 28 October 2010
Team:Edinburgh/Notebook/Red light sensor
This weekend the kanamycin resistant version of the construct (K089010 in pSB3K3) was transformed and

19 KB (2,972 words) - 02:11, 28 October 2010
Team:Tsinghua/Notebook/30 August 2010
After transformation, the trans1-T1 cells were spread onto plates with kanamycin.

211 B (32 words) - 14:49, 9 September 2010
Team:Tsinghua/Notebook/4 September 2010
Transformed trans1-T1 and spread on Kanamycin LB plates

563 B (87 words) - 21:12, 26 October 2010
Team:Tsinghua/Notebook/9 September 2010
Spread the bacteria on plates with kanamycin and chloramphenicol. Also spread double resistance bacteria (Bacteria with

333 B (43 words) - 15:13, 9 September 2010
Team:Yale/Our Project/Notebook/Week 8
...on"> standard transformation protocol </a> and a pUC19 control. Plated on kanamycin plates and left in incubator overnight. </li>

14 KB (1,289 words) - 03:50, 28 October 2010
Team:UCL London/NZY+ Broth (per Liter)
Ampicllin, Kanamycin, Tetracycline

2 KB (234 words) - 20:48, 24 October 2010
UTDallas/9 September 2010
*we transformed the ligations from Tuesday. We made 12 kanamycin and carbenicillin plates

2 KB (208 words) - 14:36, 21 September 2010
Team:UC Davis/notebook/week7.html
... plate. Hence, the cells must have kicked out the B0015 plasmid (with the kanamycin antibiotic resistance) because they don’t need it anymore. This way, we

3 KB (426 words) - 05:28, 27 September 2010
Team:Peking/Project/Biosensor/PromoterCharacterization
... two plasmids pSB3K3 and pSB1A3 were grown in LB broth with ampicillin and kanamycin at 37°C was reactivated by diluting the culture in a ratio of 1:100 with f

14 KB (2,289 words) - 03:09, 28 October 2010
Team:Peking/Parts/Characterization
... two plasmids pSB3K3 and pSB1A3 were grown in LB broth with ampicillin and kanamycin at 37°C was reactivated by diluting the culture in a ratio of 1:100 with f

75 KB (11,551 words) - 17:53, 27 October 2010
Team:WashU/Notebook/Biobricks
*12 Kanamycin and 12 tetracycline plates were made

18 KB (2,433 words) - 07:26, 27 October 2010
Construction usu.html
...e then cloned in flanking the BioBrick site, and a resistance gene (either Kanamycin resistance or Chloramphenicol resistance) was cloned in next to the BioBric

7 KB (998 words) - 00:00, 28 October 2010
Team:Cambridge/Notebook/13
I'll check in on those tomorrow. The Top10 cells are plated on kanamycin plates. If the transformation works, we can roll out the Gibson assembly. A

8 KB (1,488 words) - 23:06, 26 October 2010
Team:Harvard/vectors/agrotoplants
...s Celsius, add 150 microgarms/ml carbenicillin and either 50 microgarms/ml kanamycin for nptII selection, or 5mg/Liter <a href="http://en.wikipedia.org

4 KB (738 words) - 23:57, 26 October 2010
Team:Imperial College London/Lab Diaries/XylE team
...ells would grow up. I picked all available colonies 102 150 151 and 260 of kanamycin resistance. Lastly for assays 2x LB 2xM9 cultures were made 5ml +5ul antibi

51 KB (7,558 words) - 03:25, 28 October 2010
Team:Bielefeld-Germany/Project/Model
...px|thumb|center|'''Fig. 3: Screening construct of acetosyringone inducible kanamycin resistance read out system.''']]

6 KB (927 words) - 21:51, 27 October 2010
Team:HKUST/Project/Materials and Methods
...we used are listed as follow: ampicillin (150μg/ml for E. coli), kanamycin (50μg/ml for E. coli), chloramphenicol (35μg/ml for E. coli<

21 KB (3,038 words) - 15:14, 24 October 2010
Team:Minnesota/Protocols
Kanamycin 30 mg/ml,

25 KB (3,871 words) - 18:56, 27 October 2010
Team:BCCS-Bristol/Wetlab/adding RFP
...llet in 100mL of LB and plate out onto agar containing both Ampicillin and Kanamycin and incubate overnight at 37°C

1 KB (220 words) - 08:14, 18 October 2010
Team:MIT gateway
The resulting ENTRTM-vectors are kanamycin resistant. 

9 KB (1,391 words) - 16:54, 27 October 2010
Team:NCTU Formosa/Cry production
...n the appropriate media and antibiotic, such as agar plates with 25 µg/ml kanamycin.

15 KB (1,962 words) - 02:20, 28 October 2010
Team:UNIPV-Pavia/Project/motivation
...ncoding Chloramphenicol resistance. Other well known selection markers are Kanamycin and Tetracycline resistances.

15 KB (2,285 words) - 16:13, 24 October 2010
Team:UNIPV-Pavia/Project/references
#Bernard P (1995), New ccdB positive-selection cloning vectors with kanamycin or chloramphenicol selectable markers. Gene, Aug 30; 162(1) 159-60.

17 KB (2,176 words) - 07:10, 25 October 2010
Team:Northwestern/Protocol/Reagents
Kanamycin - Sigma K4000-5G $59.60

1 KB (218 words) - 01:23, 23 October 2010
Team:Heidelberg/Notebook/Material Methods
|Kanamycin||50 µg/ml||ddH2O

20 KB (2,928 words) - 02:14, 28 October 2010
Team:Uppsala-SwedenWeek15
...f J1, J2 and J3 assembled with BB_I0460 which contains both Ampicillin and Kanamycin cassettes in its backbone, psB1AK3, and the J- construct contains Chloramph

20 KB (2,958 words) - 19:50, 26 October 2010
Team:Peking/Notebook/Protocols/SDS&W
... 50 ul of the overnight bacterial culture to 50 ml of LB medium-containing Kanamycin (40 g/ml). Cultures were grown at 37 °C until the A600 reached 0.6 and the

2 KB (338 words) - 14:57, 25 October 2010
Team:Paris Liliane Bettencourt/Notebook/2010/07/13/
...splay:block;">-> Transformation didn't work, the product of ligation is Kanamycin resistant NOT Ampicillin resistant !!!

6 KB (911 words) - 20:07, 25 October 2010
Team:Alberta/Notebook/CreatingParts
...s (Ampicillin, Tetracycline and Chlormaphenicol) that are analogous to the kanamycin PCR fragements.

8 KB (1,148 words) - 10:51, 27 October 2010
Team:Alberta/human practices/HighSchool
... E. coli . The following day, there were red colonies present on kanamycin plates, demonstrating that their assembly was indeed successful! The studen

4 KB (681 words) - 16:43, 27 October 2010
Team:INSA-Lyon/Project/Stage3/Theory/curli promoter
<li> The Curli promoter is from the chassis E.Coli PHL1273. It is a kanamycin resistant chassis containing a plasmid with the full-length intergenic regi

5 KB (689 words) - 18:46, 27 October 2010
Team:Alberta/Notebook/protocols/labelling
| Red || Kanamycin

7 KB (1,043 words) - 02:30, 27 October 2010
Team:Baltimore US/OctGroupNotebook
and B0011. 15 ul tetracycline, 25 ul kanamycin 

8 KB (1,325 words) - 03:36, 28 October 2010
Team:Tokyo Tech/Project/Artificial Cooperation System/Cm assay
LB medium was used as liquid culture. Antibiotics (ampicillin (Amp), kanamycin (Kan), and chloramphenicol (Cm)) were dissolved in distillation water and s

6 KB (902 words) - 03:46, 28 October 2010
Team:Alberta/Kit components
| Kanamycin

2 KB (199 words) - 02:31, 28 October 2010
Team:Brown/Project/Light pattern/Workflow
...mory module (with Kan resistance). This, at first, was because of a tricky kanamycin resistance in our competent cell stock! After much time spent resolving thi

7 KB (1,164 words) - 02:36, 28 October 2010
Team:Slovenia/PROJECT/biosynthesis/carotenoids
...rst three genes (Jazz::crtE, Blues::crtB, Zif268::crtI) into a vector with kanamycin resistance (pSB4K5) and the last three (PSBII::crtY, ZnfHivC::crtO and crtZ

12 KB (1,774 words) - 01:13, 28 October 2010
Team:Tsinghua/Notebook/15 October 2010
...r plasmids to the competents. Spread to plates with both spectinomycin and kanamycin. Incubate at 30℃.

245 B (34 words) - 15:49, 27 October 2010
Team:INSA-Lyon/Project/Stage3/Strategy/Theorycurli
 The Curli promoter comes from the chassis E.Coli PHL1273. It is a kanamycin resistant chassis containing a plasmid with the full-length intergenic regi

6 KB (942 words) - 00:51, 28 October 2010