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Team:BIOTEC Dresden/Materials
From 2010.igem.org
| Plastic and Glass wares | |
|---|---|
| Micro Tubes with Caps (1.5 ml) | Sarstedt |
| PCR Plates (96 well) | Sarstedt |
| Multiply Strips (0,2ml) and Lid Chain | Nerbe Plus |
| Gene Pulser Cuvettes (0.2 cm) | BioRad |
| Falcon tubes | Falcon |
| Combitips | Eppendorf |
| Gloves | Carl Roth |
| Chemicals | |
| Phusion Polymerase | New England Biolabs |
| Taq Polymerase | New England Biolabs |
| T4 DNA Ligase | Promega M 1804 |
| Ultra Pure Water | Sigma Aldrich |
| 10x Taq buffer | New England Biolabs |
| 10x Phusion buffer | New England Biolabs |
| Antibiotics | |
| Chloramphenicol | New England Biolabs |
| Ampicillin | Sigma Aldrich |
| Kanamycin | Sigma Aldrich |
| Tetracyclin | |
| Electrocompetent and Chemicalcompetent Cells: DH5 alpha | |
| Restriction Digest | |
| AgeI | New England Biolabs |
| SpeI | New England Biolabs |
| EcoRI | New England Biolabs |
| BamHI | New England Biolabs |
| NgoMIV | New England Biolabs |
| NEB 2 Buffer | New England Biolabs |
| BSA | New England Biolabs |
| Plating and overnight cultures | |
| LB Plates (100μg/ml Ampicillin, 15μg/ml Chloramphenicol, 30μg/ml Kanamycin, 10μg/ml Tetracyclin) | |
| Bunsen burner | |
| Spreaders | |
| Purification Kits | |
| Qiagen Miniprep kit | Qiagen |
| Qiagen Gel Extraction kit | Qiagen |
| Qiagen PCR purification kit | Qiagen |
| AHL detection | |
| MF membrane, pore size 0.22μm, diam. 47mm | Sigma-Aldrich N8645 |
| Genios Pro Platereader | TECAN |
| Gel Electrophoresis | |
| 6x Gel Loading Dye | New England Biolabs |
| 1Kb Plus DNA Ladder | New England Biolabs/Invitrogen |
| TBE and TAE Buffers | |
| Ultra Agarose | Invitrogen |
