Team:Caltech/Week 5

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* Used remaining cells to inoculate new cultures of each and to extract DNA for a test digest:
* Used remaining cells to inoculate new cultures of each and to extract DNA for a test digest:
** Digested each (ideally identical) sample with EcoRI-HF and PstI, both for 1hr and for 10min.  
** Digested each (ideally identical) sample with EcoRI-HF and PstI, both for 1hr and for 10min.  
-
** The digest appears inconclusive, as a smear is visible from the top to about 3Kb, while little or no DNA is visible in the insert range (~1.5Kb).  
+
** The digest appears inconclusive, as a smear is visible from the top to about 3Kb, while little or no DNA is visible in the insert range (~1.5Kb, see below).  
 +
[[Image:PRlyTgel7-13.jpg|200px|thumb|right|Gel image for the light-lysis test digest. (7/13)]]
** Will run another gel tonight with other bricks to check efficacy of digests and will look forward to sequencing results soon.
** Will run another gel tonight with other bricks to check efficacy of digests and will look forward to sequencing results soon.

Revision as of 00:27, 14 July 2010


iGEM 2010



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Contents

Monday 7/12

  • Group meeting today!
  • Sequencing results were really crappy (quality < 30% confidence), so began 2 5mL LB cultures of the light-lysis construct (picked from the original transformation plate).

Tuesday 7/13

  • Sent off two new samples of the light-lysis construct for sequencing using the VF2 and VR primers. Results should be available tonight or tomorrow.
  • Used remaining cells to inoculate new cultures of each and to extract DNA for a test digest:
    • Digested each (ideally identical) sample with EcoRI-HF and PstI, both for 1hr and for 10min.
    • The digest appears inconclusive, as a smear is visible from the top to about 3Kb, while little or no DNA is visible in the insert range (~1.5Kb, see below).
Gel image for the light-lysis test digest. (7/13)
    • Will run another gel tonight with other bricks to check efficacy of digests and will look forward to sequencing results soon.

Wednesday 7/14

Thursday 7/15

Friday 7/16

Weekend 7/17-18

 
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