Team:Tokyo Tech/Parts

From 2010.igem.org

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{{Tokyo_Tech_Template2}}
{{Tokyo_Tech_Template2}}
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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<div id="super_main_wrapper">
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!align="center"|[[Team:Tokyo_Tech|Home]]
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<div id="SubWrapper"
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!align="center"|[[Team:Tokyo_Tech/Team|Team]]
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<!--  ここから書き始めて下さい-->
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Tokyo_Tech Official Team Profile]
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!align="center"|[[Team:Tokyo_Tech/Project|Project]]
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!align="center"|[[Team:Tokyo_Tech/Parts|Parts Submitted to the Registry]]
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!align="center"|[[Team:Tokyo_Tech/Modeling|Modeling]]
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==New parts which were characterized and worked as designed==
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!align="center"|[[Team:Tokyo_Tech/Notebook|Notebook]]
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'''Apple Reporter'''
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!align="center"|[[Team:Tokyo_Tech/Safety|Safety]]
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{| style="margin: 1em auto 1em auto" width="600px"
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|- style="background-color:#4169e1;"  
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!Name||Part type||Registry Link
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|- style="background-color:#f5fffa;"  
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|MpAAT1||Generator||<partinfo>BBa_K395602</partinfo>
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|- style="background-color:#f5fffa;"  
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|rbs-crtW||Translational unit||<partinfo>BBa_K395705</partinfo>
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|- style="background-color:#f5fffa;"  
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|pBad/araC-crtZW||Generator||<partinfo>BBa_K395706</partinfo>
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|-
|}
|}
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'''Artificial Cooperation System'''
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{| style="margin: 1em auto 1em auto" width="600px"
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|- style="background-color:#4169e1;"
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!Name||Part type||Registry Link
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|- style="background-color:#f5fffa;"
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|(PluxR rep weak-GFP) improved||Reporter||<partinfo>BBa_K395105</partinfo>
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|- style="background-color:#f5fffa;"
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|PluxR act-Cm||signalling||<partinfo>BBa_K395162</partinfo>
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|-
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|}
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'''"Wolfcoli"zation'''
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{| style="margin: 1em auto 1em auto" width="600px"
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|- style="background-color:#4169e1;"
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!Name||Part type||Registry Link
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|- style="background-color:#f5fffa;"
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|PompC(CB)-GFP improved||Reporter||<partinfo>BBa_K395305</partinfo>
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|- style="background-color:#f5fffa;"
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|PompC(CS1)-GFP||Reporter||<partinfo>BBa_K395306</partinfo>
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|-
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|}
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===Index===
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==New parts which were characterized and did not worked as designed==
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'''"Wolfcoli"zation'''
 +
{| style="margin: 1em auto 1em auto" width="600px"
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|- style="background-color:#4169e1;"
 +
!Name||Part type||Registry Link
 +
|- style="background-color:#f5fffa;"
 +
|PompC(C)-GFP ||Reporter||<partinfo>BBa_K395304</partinfo>
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|- style="background-color:#f5fffa;"
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|PompC(WT)-GFP ||Reporter||<partinfo>BBa_K395307</partinfo>
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|-
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|}
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New for iGEM 2010 is the ''groupparts'' tag.  This tag will generate a table with all of the parts that your team adds to your team sandbox.  Note that if you want to document a part you need to document it on the [http://partsregistry.org Registry], not on your team wiki.
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==Existing parts which were characterized through our activity==
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'''Apple Reporter '''
 +
 +
{| style="margin: 1em auto 1em auto" width="600px"
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|- style="background-color:#4169e1;"
 +
!Name||Part type||Registry Link
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|- style="background-color:#f5fffa;"
 +
|crtZ (beta-carotene hydroxylase) coding sequence ||Coding||<partinfo>BBa_I742157</partinfo>
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|- style="background-color:#f5fffa;"
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|crtZ (beta-carotene hydroxylase) with native rbs ||Translational Unit||<partinfo>BBa_I742158</partinfo>
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|-
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|}
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 +
''In order to characterize these parts, following 3 improved parts were constructed.''
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 +
:::::<br>(Name,Parts,registry Number)
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:::::<br>(placIQ-crtZ ,Generator,<partinfo>BBa_K395701</partinfo>)
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:::::<br>(pBad/araC-crtZ ,Generator,<partinfo>BBa_K395702</partinfo>)
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:::::<br>(pBad/araC-crtZ-crtEBIY ,Generator,<partinfo>BBa_K395704</partinfo>)
 +
 +
 +
 +
 +
'''“Wolfcoli”zation  '''
 +
 +
{| style="margin: 1em auto 1em auto" width="600px"
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|- style="background-color:#4169e1;"
 +
!Name||Part type||Registry Link
 +
|- style="background-color:#f5fffa;"
 +
|LacI-Mutant ||Coding||<partinfo>BBa_K082005</partinfo>
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|- style="background-color:#f5fffa;"
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|LacIM1-B0015||Composite||<partinfo>BBa_K082016</partinfo>
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|- style="background-color:#f5fffa;"
 +
|B0034-LacIM1-B0015||Generator||<partinfo>BBa_K082026</partinfo>
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|-
 +
|}
 +
 +
''In order to characterize these parts, following 2 improved parts were constructed.''
 +
 +
:::::<br>(Name,Parts,registry Number)
 +
:::::<br>(Pbad/araC-lacIM1 ,Composite,<partinfo>BBa_K395401</partinfo>)
 +
:::::<br>(Pbad/araC-lacI ,Composite,<partinfo>BBa_K395402</partinfo>)
 +
 +
 +
'''Artificial Cooperation System'''
 +
{| style="margin: 1em auto 1em auto" width="600px"
 +
|- style="background-color:#4169e1;"
 +
!Name||Part type||Registry Link
 +
|- style="background-color:#f5fffa;"
 +
|PluxR rep||Regulatory||<partinfo>BBa_R0061</partinfo>
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|-
 +
|}
 +
''In order to characterize this part, following improved part was constructed.''
 +
:::::<br>(Name,Parts,registry Number)
 +
:::::<br>(GFP reporter repressed by LuxR and 3OC6HSL ,Reporter,<partinfo>BBa_K395101</partinfo>)
 +
 +
{| style="margin: 1em auto 1em auto" width="600px"
 +
|- style="background-color:#4169e1;"
 +
!Name||Part type||Registry Link
 +
|- style="background-color:#f5fffa;"
 +
|PluxR act||Regulatory||<partinfo>BBa_R0062</partinfo>
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|-
 +
|}
 +
 +
''In order to characterize this part, following part was constructed.''
 +
:::::<br>(Name,Parts,registry Number)
 +
:::::<br>(GFP reporter activated by LuxR and 3OC6HSL,Reporter,<partinfo>BBa_K395100</partinfo>)
 +
 +
==All of the parts we registered==
<groupparts>iGEM010 Tokyo_Tech</groupparts>
<groupparts>iGEM010 Tokyo_Tech</groupparts>
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===Further Information===
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 +
<!-- ココより下はいじっちゃやーよ-->
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</div> <!-- end SubWrapper -->
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<div id="news">
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</div>  <!-- end news -->
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</div> <!-- end Super_main_wrapper -->
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</body></html>

Latest revision as of 22:58, 27 October 2010

iGEM Tokyo Tech 2010 "E.coli with Humanity"


New parts which were characterized and worked as designed

Apple Reporter

NamePart typeRegistry Link
MpAAT1Generator<partinfo>BBa_K395602</partinfo>
rbs-crtWTranslational unit<partinfo>BBa_K395705</partinfo>
pBad/araC-crtZWGenerator<partinfo>BBa_K395706</partinfo>

Artificial Cooperation System

NamePart typeRegistry Link
(PluxR rep weak-GFP) improvedReporter<partinfo>BBa_K395105</partinfo>
PluxR act-Cmsignalling<partinfo>BBa_K395162</partinfo>

"Wolfcoli"zation

NamePart typeRegistry Link
PompC(CB)-GFP improvedReporter<partinfo>BBa_K395305</partinfo>
PompC(CS1)-GFPReporter<partinfo>BBa_K395306</partinfo>

New parts which were characterized and did not worked as designed

"Wolfcoli"zation

NamePart typeRegistry Link
PompC(C)-GFP Reporter<partinfo>BBa_K395304</partinfo>
PompC(WT)-GFP Reporter<partinfo>BBa_K395307</partinfo>

Existing parts which were characterized through our activity

Apple Reporter

NamePart typeRegistry Link
crtZ (beta-carotene hydroxylase) coding sequence Coding<partinfo>BBa_I742157</partinfo>
crtZ (beta-carotene hydroxylase) with native rbs Translational Unit<partinfo>BBa_I742158</partinfo>

In order to characterize these parts, following 3 improved parts were constructed.


(Name,Parts,registry Number)

(placIQ-crtZ ,Generator,<partinfo>BBa_K395701</partinfo>)

(pBad/araC-crtZ ,Generator,<partinfo>BBa_K395702</partinfo>)

(pBad/araC-crtZ-crtEBIY ,Generator,<partinfo>BBa_K395704</partinfo>)



“Wolfcoli”zation

NamePart typeRegistry Link
LacI-Mutant Coding<partinfo>BBa_K082005</partinfo>
LacIM1-B0015Composite<partinfo>BBa_K082016</partinfo>
B0034-LacIM1-B0015Generator<partinfo>BBa_K082026</partinfo>

In order to characterize these parts, following 2 improved parts were constructed.


(Name,Parts,registry Number)

(Pbad/araC-lacIM1 ,Composite,<partinfo>BBa_K395401</partinfo>)

(Pbad/araC-lacI ,Composite,<partinfo>BBa_K395402</partinfo>)


Artificial Cooperation System

NamePart typeRegistry Link
PluxR repRegulatory<partinfo>BBa_R0061</partinfo>

In order to characterize this part, following improved part was constructed.


(Name,Parts,registry Number)

(GFP reporter repressed by LuxR and 3OC6HSL ,Reporter,<partinfo>BBa_K395101</partinfo>)
NamePart typeRegistry Link
PluxR actRegulatory<partinfo>BBa_R0062</partinfo>

In order to characterize this part, following part was constructed.


(Name,Parts,registry Number)

(GFP reporter activated by LuxR and 3OC6HSL,Reporter,<partinfo>BBa_K395100</partinfo>)

All of the parts we registered

<groupparts>iGEM010 Tokyo_Tech</groupparts>


</body></html>