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Protocol for Chemical Inducible Expression of GFP


  • 4 groups of induce solution with a concentration gradient of 10^-7, 10^-5, 10^-3, 10^-2;
  • Overnight bacterial culture or bacterial colonies;
  • Phosphate Buffered Solution (PBS).


1. Add 20 μl of the overnight bacteria l culture or pick a colony to 5ml of LB antibiotic medium, Incubate at 37 degree in a shaker till the OD600 value reaches 0.4-0.6.

2. Add 0.5 mL of the fresh bacteria l culture and appropriate volume of inducer solution to prepare induction system with the concentration gradient of 10^-9, 10^-8, 10^-7, 10^-6, 10^-5, 10^-4, 10^-3, 10^-2.

3. Place the induction system at 37 degree for 2 hours.

4. Pellet bacteria l cells by 4 min centrifugation at 4000 rpm, discard the supernatant.

5. Resuspend the pelleted cells in 500 μl of PBS.

6. Transfer 100 uL of bacteria l resuspention into each well of 96-well plate to test the expression of GFP by flow cytometry or Microplate Reader.


If desired, time sequential expression of GFP can also be tested, through verifying the incubating time of induction system at 37 degree.

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