Team:Tsinghua/experiments

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              <strong>HOME</strong>TSINGHUA iGEM</span>  
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<h1>Experiment Records</h1>
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              <strong>BACKGROUND</strong>ANTIBODY MANUFACTURE</span>
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              <strong>PROJECT</strong>PLAN & ACTION</span>
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<p>Our Experiments were carried out by the following nine groups which are somewhat independent from each other. Here come the Groups and Their Tasks.</p>
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              <strong>EXPERIMENTS</strong>PROGRESS UP-TO-DATE</span>
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Group 1A：Landing pad (LP) construction and Insertion with Tranditional Att Recomination method(Promoter Inside)<br/>
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Group 1B：Landing pad (LP) construction and Insertion with Innovational Att Recomination method(Promoter Outside)<br/>
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Group 1C：Landing pad (LP) construction and Insertion: Inducement and Recombination
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              <strong>TEAM</strong>ABOUT US</span>
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Group 2A：Donor Plasmid Construction: Two Fragments Construction (Three Fragments Ligation)
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Group 2B：Donor Plasmid Construction: Traditional Four Fragments Construction
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Group 2C：Donor Plasmid Construction: Four Fragments Construction with Dra III
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              <strong>PARTS</strong>STD BIOBRICKS</span>
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Group 2D：Donor Plasmid Construction: Four Fragments Construction with Novel Methods
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Group 3：Helper Plasmid (HP) Insertion, Donor Plasmid Insertion, Recombination Induction & Removal of HP
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Group 4：Screening Strategy
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<h1>Protocols</h1>
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Here are our Experimental Protocols in PDF version. You're advised to view in <a href="http://www.adobe.com/acom/" target=blank>Acrobat</a> or <a href="http://get.adobe.com/reader/" target=blank>Adobe Reader</a> which you can download free from the<a href="http://www.adobe.com" target=blank> Home Website of Adobe® Company</a>, for other viewers we can not guarantee the right format of viewing. Those files are encrypted to keep copyrights, if you want to print them out of copy to your documents, to share with others our ideas and methods, you are welcome to ask us for the unencrypted files definitely for free~
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<h3>Molecular Cloning</h3>
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<p><a href="https://static.igem.org/mediawiki/2010/0/0a/THUProtocol_1-1_Isolatio_of_plasmid_DNA.pdf" target=blank>THUProtocol_1-1_Isolatio_of_plasmid_DNA</a><br>
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</p><p>
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<a href="https://static.igem.org/mediawiki/2010/d/d8/THUProtocol_1-2_DNA_Gel_Extraction.pdf" target=blank>THUProtocol_1-2_DNA_Gel_Extraction</a>
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<a href="https://static.igem.org/mediawiki/2010/5/59/THUProtocol_1-3_Restriction_Enzyme_Digestion.pdf" target=blank>THUProtocol_1-3_Restriction_Enzyme_Digestion</a>
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</p><p>
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<a href="https://static.igem.org/mediawiki/2010/b/b7/THUProtocol_1-4_Ligation_of_DNA_Fragments.pdf" target=blank>THUProtocol_1-4_Ligation_of_DNA_Fragments</a>
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<a href="https://static.igem.org/mediawiki/2010/c/c3/THUProtocol_1-5_PCR.pdf" target=blank>THUProtocol_1-5_PCR</a>
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<a href="https://static.igem.org/mediawiki/2010/c/c0/THUProtocol_1-6_Preparation_of_Competent_Cell_for_Electro_Transformation.pdf" target=blank>THUProtocol_1-6_Preparation_of_Competent_Cell_for_Electro_Transformation</a>
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</p><p>
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<a href="https://static.igem.org/mediawiki/2010/7/77/THUProtocol_1-7_Chemical_Transformation_of_Recombinant_DNA.pdf" target=blank>THUProtocol_1-7_Chemical_Transformation_of_Recombinant_DNA</a>
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</p><p>
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<a href="https://static.igem.org/mediawiki/2010/4/40/THUProtocol_1-8_Electro_Transformation_of_Recombinant_DNA.pdf" target=blank>THUProtocol_1-8_Electro_Transformation_of_Recombinant_DNA</a>
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</p><p>
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<h3>Protein Isolation and Identification</h3>
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<a href="https://static.igem.org/mediawiki/2010/f/f0/THUProtocol_2-1_Protein_Isolation_for_Prokaryotes.pdf" target=blank>THUProtocol_2-1_Protein_Isolation_for_Prokaryotes</a>
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</p><p>
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<a href="https://static.igem.org/mediawiki/2010/0/08/THUProtocol_2-2_Protein_Identification_SDS-PAGE.pdf" target=blank>THUProtocol_2-2_Protein_Identification_SDS-PAGE</a>
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</p><p>
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<a href="https://static.igem.org/mediawiki/2010/c/c3/THUProtocol_1-5_PCR.pdf" target=blank>THUProtocol_1-5_PCR</a>
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Latest revision as of 17:40, 27 October 2010

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Experiment Records

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Our Experiments were carried out by the following nine groups which are somewhat independent from each other. Here come the Groups and Their Tasks.

Group 1A：Landing pad (LP) construction and Insertion with Tranditional Att Recomination method(Promoter Inside)

Group 1B：Landing pad (LP) construction and Insertion with Innovational Att Recomination method(Promoter Outside)

Group 1C：Landing pad (LP) construction and Insertion: Inducement and Recombination

Group 2A：Donor Plasmid Construction: Two Fragments Construction (Three Fragments Ligation)

Group 2B：Donor Plasmid Construction: Traditional Four Fragments Construction

Group 2C：Donor Plasmid Construction: Four Fragments Construction with Dra III

Group 2D：Donor Plasmid Construction: Four Fragments Construction with Novel Methods

Group 3：Helper Plasmid (HP) Insertion, Donor Plasmid Insertion, Recombination Induction & Removal of HP

Group 4：Screening Strategy

Protocols

Here are our Experimental Protocols in PDF version. You're advised to view in Acrobat or Adobe Reader which you can download free from the Home Website of Adobe® Company, for other viewers we can not guarantee the right format of viewing. Those files are encrypted to keep copyrights, if you want to print them out of copy to your documents, to share with others our ideas and methods, you are welcome to ask us for the unencrypted files definitely for free~

Molecular Cloning

THUProtocol_1-1_Isolatio_of_plasmid_DNA

THUProtocol_1-2_DNA_Gel_Extraction

THUProtocol_1-3_Restriction_Enzyme_Digestion

THUProtocol_1-4_Ligation_of_DNA_Fragments

THUProtocol_1-5_PCR

THUProtocol_1-6_Preparation_of_Competent_Cell_for_Electro_Transformation

THUProtocol_1-7_Chemical_Transformation_of_Recombinant_DNA

THUProtocol_1-8_Electro_Transformation_of_Recombinant_DNA

Protein Isolation and Identification

THUProtocol_2-1_Protein_Isolation_for_Prokaryotes

THUProtocol_2-2_Protein_Identification_SDS-PAGE

THUProtocol_1-5_PCR

Retrieved from "http://2010.igem.org/Team:Tsinghua/experiments"