Team:Tsinghua/Notebook/8 October 2010

From 2010.igem.org

Module I, Group 1A

Extract plasmid ptv. Transform into competent with pAH69 through electrotransformation. Spread to 0.03% tetracyclin plates.

Module I, Group 2c

Digest the purified products and plasmid PSB1C13 with EcoR I and Pst I.


Double digestion system

eGFP                 5ul
EcoR I               1ul
Pst I                1ul
FD Buffer            2ul
H2O                  11ul

mCherry              4ul
EcoR I               1ul
Pst I                1ul
FD Buffer            2ul
H2O                  12ul

Kan                  4ul
EcoR I               1ul
Pst I                1ul
FD Buffer            2ul
H2O                  12ul

Incubate at 37 centidegree overnight.