Team:Tokyo Tech/Project/wolf coli


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iGEM Tokyo Tech 2010 "E.coli with Humanity"

4 Wolf coli. Overview


Figure3-1. Introduction of “Wolf coli.”

Regulation of Artificial Cooperation System (ACS)lead to alternative behavioral of E. coli.Our team challenged to design “Wolfcoli”, an E coli. that its behavior changes due to the intensity of lightinspired by famous legend of wolfman. When there is intermediate light intensity, two cells doesn’t help each other at only full moon night .  From these behaviors, we call this “Wolfcoli” (Fig. 3-1).

Figure3-2. Overview of “Wolfcoli” system

In order to create the “Wolf coli.”, we introduced " red-light-dependent gene expression network"[1] and "band-detect network"[2] and combined these networks with the Artificial Cooperation System (Fig. 3-2). We conducted experiment on series of OmpC promoter (PompC), and LacIM1 which are crucial parts of our networks.


Figure3-3. The induction of new P’’ompC’’ series in high osmolarity medium at hours. This work is done by Thiprampai THAMAMONGOOD and Taichi NAKAMURA
Figure3-4.Repression efficiency of LacIM1 (BBa_K395401) / LacIWT (BBa_K395402) exposed to arabinose and IPTG. This work is done by Mitsuhiko ODERA

We succeeded in designing 2 new osmoregulative promoters, PompC(CB) (BBa_K395302) and PompC(CS1) (BBa_K395303), which can be utilized in the red-light-dependent gene expression network (Fig. 3-3). We characterized lacIM1 (BBa_K082026), a mutation of LacI (BBa_?????),which is a key component in the band-detect network. In fact, this part was registered by USTC(2008) [3], however it was not well characterized in the BioBrick registry. We found that product of lacIM1 shows weaker repression to Plac than its wild type. (Fig. 3-4)