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Revision as of 11:43, 19 October 2010 (view source) Mizuki (Talk | contribs) (→4:Transformation) ← Older edit		Revision as of 11:43, 19 October 2010 (view source) Mizuki (Talk | contribs) (→5:Electrophoresis) Newer edit →
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	<Sample><br />		<Sample><br />
	PCR products		PCR products
-	*1 (9/28)	+	*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 1] (9/28)
-	*2 (9/28)	+	*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 2] (9/28)
-	*4 (9/28)	+	*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 4] (9/28)
-	*5 (9/28)	+	*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 5] (9/28)
-	*8 (9/27)	+	*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 8] (9/27)
-	*9 (9/28)	+	*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 9] (9/28)
-	*10(9/28)	+	*[https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Sample_Number/ 10](9/28)
	*vector		*vector

---

Revision as of 11:43, 19 October 2010

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Acknowledgment

Contents 1 2010/09/28 1.1 1：DNA extraction 1.2 2：DNA Ligation 1.3 3:PCR 1.4 4:Transformation 1.5 5:Electrophoresis 1.6 6：DNA extraction 1.7 7：DNA Digestion 1.8 8:PCR

2010/09/28

1：DNA extraction

＜Member＞
mizuki,Takahiro

＜Sample＞
PCR products

• 1(9/16)
• 2(9/16)
• 3(9/17)
• 4(9/16)
• 5(9/16)
• 7(9/16)

＜Protocol＞
See Protocol 4

2：DNA Ligation

＜Member＞
mio

＜Sample＞

• 7-8-10,vector
• 2,vector

(after digestion)

＜Protocol＞
See Protocol 3

3:PCR

<Member>
mizuki,Takahiro

<Sample>(temperature in annealing:℃)

• 1 (72)
• 2 (70)
• 4 (70)
• 5 (70)
• 9 (63,68)
• 10(58)
• vector(72)

<Protocol>
See Protocol 1

4:Transformation

<Member>
nito

<Sample>

• 7-8-10+vector
• 2+vector

<Protocol>
See Protocol 7

5:Electrophoresis

<Member>
nito,Takahiro

<Sample>
PCR products

• 1 (9/28)
• 2 (9/28)
• 4 (9/28)
• 5 (9/28)
• 8 (9/27)
• 9 (9/28)
• 10(9/28)
• vector

<Protocol>
See Protocol 8

<Result>

6：DNA extraction

＜Member＞
Takahiro

＜Sample＞
PCR products

＜Protocol＞
See Protocol 4

7：DNA Digestion

＜Member＞
Mariko

＜Sample, Materials＞
・PCR productions

• 1 (8/31)
• 2 (9/11)
• 4L(8/26)
• 5 (8/31)

・Digest enzyme

• Avr2
• Nhe1
• spe1

＜Protocol＞
See Prptpcpl 9

8:PCR

<Member>
Mariko

<Sample>(temperature in annealing:℃)

• 1 (72)
• 2 (70)
• 3 (69)
• 4 (70)

<Protocol>
See Protocol 1