Team:TU Delft/Modeling/MFA


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Metabolic Flux Analysis

A metabolic flux analysis (MFA) is an analysis to calculate the theoretical maximal yields for a proposed system of pathways in a micro-organism. Several product pathways were introduced to E. coli along with the hydrocarbon degradation from our BioBricks to see what the maximal theoretical yields on alkanes are compared to glucose.

Are you not familiar with MFAs? Read more about how a metabolic flux analysis works.

For our MFA a Matlab application, Cell Net Analyzer, was used. CNA already contains a standard simplified E. coli metabolic network. This network was slightly changed and hydrocarbon degradation was added to it. The resulting network is the network used for adding additional pathways.

Implemented Pathways

Would you like to more about the details of the implemented pathways? Read the Detailed MFA results page.

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Visualization of pathways