Team:Paris Liliane Bettencourt/test home

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<br>Our second counter operates on the wholly different principle that the statistical occurrence of a rare event in a large population can be modeled.  Each cell in our population harbors a construct that when stimulated has a small chance of excising a terminator and expressing a resistance gene.  The number of resistant cells is thus an accurate count of the number of input stimuli.
<br>Our second counter operates on the wholly different principle that the statistical occurrence of a rare event in a large population can be modeled.  Each cell in our population harbors a construct that when stimulated has a small chance of excising a terminator and expressing a resistance gene.  The number of resistant cells is thus an accurate count of the number of input stimuli.
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<div align="center">
 
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<a href="https://2010.igem.org/Image:Populationcounter1.jpg" onMouseOut="initial()" onMouseOver="i_change()" >
 
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<img src="https://static.igem.org/mediawiki/2010/thumb/c/cd/Populationcounter1.jpg/600px-Populationcounter1.jpg" border="0" name="image" />
 
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<script language="JavaScript">
 
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var i_width=515;
 
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var i_height=385;
 
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document.image.width = Math.round(i_width/coeff);
 
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document.image.height = Math.round(i_height/coeff);
 
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<br /><br />This counter, like the first one, can also be used in “timer” mode.  Timer functionality is achieved by co-expressing with the antibiotic gene LuxI, under a promoter of our choice.  When the media concentration of AHL exceeds a threshold (in part determined by the promoter we choose) YFG is expressed.  To be able to count correctly, this system requires careful control of the media, so we have designed a microfluidic device to test this system.
 

Revision as of 10:57, 17 September 2010


Abstract


Counting is the action of finding the number of elements in a set. Past attempts at developing counters in cells have mostly attempted to mimic the binary methods that computers use to count.
Our first counter takes a new approach to counting in cells, essentially a mechanical rotary counter implemented on a micro scale. Each time the counter detects an input, it performs an excision and integration directly down-stream of the active site, turning on a reporter and rotating over one "notch" on the counter.
Our second counter operates on the wholly different principle that the statistical occurrence of a rare event in a large population can be modeled. Each cell in our population harbors a construct that when stimulated has a small chance of excising a terminator and expressing a resistance gene. The number of resistant cells is thus an accurate count of the number of input stimuli.

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