Team:Newcastle/20 August 2010

From 2010.igem.org

(Difference between revisions)
(Gel Extraction)
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==Materials and Protocol==
==Materials and Protocol==
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Please refer to [[Team:Newcastle/Gel_extraction|gel extraction]].
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Please refer to [[Team:Newcastle/Gel_extraction|gel extraction]] and [[TeamNewcastleNanoDrop_Spectrophotometer|nanodrop]].
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==Results==
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==Discussion==
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==Conclusion==
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=Transformation of Ligated Products=
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==Aims==
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To produce more colonies of the ligated products of ''yneA'' with pGFPrrnB and pSB1C3.
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==Materials and Protocol==
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Please refer to [[Team:Newcastle/Transformation_of_E._coli|transformation of E. coli]].
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Revision as of 11:07, 20 August 2010

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Contents

Aims

Following yesterday's Phusion PCR, we plan to first perform gel electrophoresis, then gel extraction, and finally nanodrop to record the level of DNA.

Materials and protocol

Please refer to the gel electrophoresis, gel extraction and NanoDrop protocols. Vacuum manifold will be used.


Gel Extraction

Aims

To purify the PCR products of digested yneA, pGFPrrnB and pSB1C3 from yesterday.

Materials and Protocol

Please refer to gel extraction and nanodrop.

Results

Discussion

Conclusion

Transformation of Ligated Products

Aims

To produce more colonies of the ligated products of yneA with pGFPrrnB and pSB1C3.

Materials and Protocol

Please refer to transformation of E. coli.






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