Team:Newcastle/18 August 2010

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Contents

Miniprep of filamentous cell part

Aims

The aim of the experiment is to prepare stocks of the plasmid DNA containing our filamentous cell part.

Materials and Protocol

Please refer to protocols mentioned below for materials required:

Results

yneA 1 yneA 2 yneA 3 yneA 4
Concentration of DNA ng/µl 247.3 233.6 456.6 140.0

Table 1: Nanodrop spectrophotometer results. Table represents the amount of plasmid present in µl/ml quantity.

Nanodrop1882010.jpeg Figure 1: Screenshot of yneA tube 1 Nanodrop18820102.jpeg Figure 2: Screenshot of yneA tube 2 Nanodrop18820103.jpeg Figure 3: Screenshot of yneA tube 3 Nanodrop18820104.jpeg Figure 4: Screenshot of yneA tube 4

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Discussion

The concentration of the different tubes range from 140.0 µl/ml to 456.6 µl/ml. The standard value for miniprep extraction is ~150 µg/ml.

Conclusion

High DNA concentration was obtained.

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