Warning: mkdir(): Permission denied in /websites/2010.igem.org/wiki/includes/GlobalFunctions.php on line 2176

Warning: wfMkdirParents: failed to mkdir "/websites/2010.igem.org/wiki/images/thumb/1/1f/Caltech_logo.png" mode 511 in /websites/2010.igem.org/wiki/includes/GlobalFunctions.php on line 2179
Team:Caltech/Week 13 - 2010.igem.org
 

Team:Caltech/Week 13

From 2010.igem.org

(Difference between revisions)
(New page: {{Caltech_iGEM_10| Content= {{:Team:Caltech/WeekTemplate}} ==Monday 9/6== ==Tuesday 9/7== ==Wednesday 9/8== ==Thursday 9/9== ==Friday 9/10==   }})
 
(2 intermediate revisions not shown)
Line 5: Line 5:
==Monday 9/6==
==Monday 9/6==
 +
*Colony PCR cultures of L95 (HSP-LacI-EYFP) cells imaged under microscope
 +
**Cells are vastly overgrown and unhealthy (cultures visualized after one weekend)
 +
*Fresh cultures of L95 cells grown in preparation for a proper microscope imaging session
==Tuesday 9/7==
==Tuesday 9/7==
 +
*Digested successful sigma32 (HtpR) BioBrick in preparation for ligation into pSB1C3 chloramphenicol backbone
 +
**Treat the digestion like a backbone digestion
 +
*Ligated the digest into pSB1C3
 +
*Cleaned up the lab in preparation for mandatory leave of absence
==Wednesday 9/8==
==Wednesday 9/8==
 +
*Photographed the results of transglutaminase long-term testing (6 week span)
 +
**Results are stunning: TGase + cells, TGase + cell lysate forms a dark grey-colored brick about 7mm diameter (culture tube was approximately 13-14mm inside diameter!)
 +
**Several TGase + acid and TGase + base show very dark, vivid colors unattributable to the acid/base
 +
*Mandatory leave of absence begins
==Thursday 9/9==
==Thursday 9/9==
-
 
+
The lab is being used by a class.
==Friday 9/10==
==Friday 9/10==
-
 
+
The lab is being used by a class.
 
 
}}
}}

Latest revision as of 16:02, 17 October 2010


iGEM 2010



Home

People

Project Details

Protocols

Completed Systems

Notebook

Biosafety

Human Impact

References

Support


Caltech logo watermark.png


Monday 9/6

  • Colony PCR cultures of L95 (HSP-LacI-EYFP) cells imaged under microscope
    • Cells are vastly overgrown and unhealthy (cultures visualized after one weekend)
  • Fresh cultures of L95 cells grown in preparation for a proper microscope imaging session

Tuesday 9/7

  • Digested successful sigma32 (HtpR) BioBrick in preparation for ligation into pSB1C3 chloramphenicol backbone
    • Treat the digestion like a backbone digestion
  • Ligated the digest into pSB1C3
  • Cleaned up the lab in preparation for mandatory leave of absence

Wednesday 9/8

  • Photographed the results of transglutaminase long-term testing (6 week span)
    • Results are stunning: TGase + cells, TGase + cell lysate forms a dark grey-colored brick about 7mm diameter (culture tube was approximately 13-14mm inside diameter!)
    • Several TGase + acid and TGase + base show very dark, vivid colors unattributable to the acid/base
  • Mandatory leave of absence begins

Thursday 9/9

The lab is being used by a class.

Friday 9/10

The lab is being used by a class.

 
Error creating thumbnail: Unable to create destination directory
Caltech footer.png


Locations of visitors to this page