Team:Caltech/Week 13
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==Monday 9/6== | ==Monday 9/6== | ||
+ | *Colony PCR cultures of L95 (HSP-LacI-EYFP) cells imaged under microscope | ||
+ | **Cells are vastly overgrown and unhealthy (cultures visualized after one weekend) | ||
+ | *Fresh cultures of L95 cells grown in preparation for a proper microscope imaging session | ||
==Tuesday 9/7== | ==Tuesday 9/7== | ||
+ | *Digested successful sigma32 (HtpR) BioBrick in preparation for ligation into pSB1C3 chloramphenicol backbone | ||
+ | **Treat the digestion like a backbone digestion | ||
+ | *Ligated the digest into pSB1C3 | ||
+ | *Cleaned up the lab in preparation for mandatory leave of absence | ||
==Wednesday 9/8== | ==Wednesday 9/8== | ||
+ | *Photographed the results of transglutaminase long-term testing (6 week span) | ||
+ | **Results are stunning: TGase + cells, TGase + cell lysate forms a dark grey-colored brick about 7mm diameter (culture tube was approximately 13-14mm inside diameter!) | ||
+ | **Several TGase + acid and TGase + base show very dark, vivid colors unattributable to the acid/base | ||
+ | *Mandatory leave of absence begins | ||
==Thursday 9/9== | ==Thursday 9/9== | ||
- | + | The lab is being used by a class. | |
==Friday 9/10== | ==Friday 9/10== | ||
- | + | The lab is being used by a class. | |
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Latest revision as of 16:02, 17 October 2010
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Week 1 | Week 2 | Week 3 | Week 4 | Week 5 | Week 6 | Week 7 | Week 8 | Week 9 | Week 10 | Week 11 | Week 12 | Week 13 | Week 14 | Week 15
Monday 9/6
Tuesday 9/7
Wednesday 9/8
Thursday 9/9The lab is being used by a class. Friday 9/10The lab is being used by a class.
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