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Team:Calgary/31 July 2010
From 2010.igem.org
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'''Saturday July 31, 2010''' | '''Saturday July 31, 2010''' | ||
| + | [[Image:07.31.2010-CpxPPurified.jpg|thumb|400px|Chris's gel of the CpxP promoter PCR product purified using a Qiagen kit. Lanes 1 and 2 are Tube 1, Lanes 3 and 4 are Tube 2, Lanes 5 and 6 are Tube 3.]] | ||
We love weekends!! Except when we come in to work... | We love weekends!! Except when we come in to work... | ||
<u>Chris</u> | <u>Chris</u> | ||
| - | Today, I did a PCR purification of the CpxP promoter that was PCR-ed out of the E. coli genome using the Qiagen kit that we have. 100 µL of each PCR tube was purified. Emily and I took a look at the vacuum PCR purification that Henry provided but were unsure of the protocol to use. One modification to the protocol that was used was the addition of 15 minutes waiting time in between the washing and the eluting step of the protocol. This was suggested by another lab as a method to increase DNA concentration in the final product. Another modification to the procedure was that the elution was done in 35 µL of water instead of the PB Buffer. | + | Today, I did a PCR purification of the CpxP promoter that was PCR-ed out of the E. coli genome using the Qiagen kit that we have. 100 µL of each PCR tube was purified. Emily and I took a look at the vacuum PCR purification that Henry provided but were unsure of the protocol to use. One modification to the protocol that was used was the addition of 15 minutes waiting time in between the washing and the eluting step of the protocol. This was suggested by another lab as a method to increase DNA concentration in the final product. Another modification to the procedure was that the elution was done in 35 µL of water instead of the PB Buffer. A gel was run of the purified PCR product and the gel can be seen on the right. The bands are still about the same place which they should be for the CpxP promoter. |
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Revision as of 00:03, 1 August 2010
Saturday July 31, 2010
Chris's gel of the CpxP promoter PCR product purified using a Qiagen kit. Lanes 1 and 2 are Tube 1, Lanes 3 and 4 are Tube 2, Lanes 5 and 6 are Tube 3.
We love weekends!! Except when we come in to work...
Chris
Today, I did a PCR purification of the CpxP promoter that was PCR-ed out of the E. coli genome using the Qiagen kit that we have. 100 µL of each PCR tube was purified. Emily and I took a look at the vacuum PCR purification that Henry provided but were unsure of the protocol to use. One modification to the protocol that was used was the addition of 15 minutes waiting time in between the washing and the eluting step of the protocol. This was suggested by another lab as a method to increase DNA concentration in the final product. Another modification to the procedure was that the elution was done in 35 µL of water instead of the PB Buffer. A gel was run of the purified PCR product and the gel can be seen on the right. The bands are still about the same place which they should be for the CpxP promoter.
No notebook page exists for this date. Sorry!
