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Team:Calgary/24 June 2010
From 2010.igem.org
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Today, I restriction digested the construction of E0040+B0015 that was constructed on Monday. This would help identify the identity of the construct. I expected to see bands in the 3000 and 800 range however, bands were found in the | Today, I restriction digested the construction of E0040+B0015 that was constructed on Monday. This would help identify the identity of the construct. I expected to see bands in the 3000 and 800 range however, bands were found in the | ||
4000-3000 range which could be uncut plasmids | 4000-3000 range which could be uncut plasmids | ||
| + | [[Image:06.24.2010.E0040+B0015RestrictionDigest(2).jpg|thumb|400px|Dev's gel of restriction digest of E0040+B0015]] | ||
}} | }} | ||
Revision as of 22:01, 7 July 2010
Thursday June 24, 2010
Emily
Today primers came in for the maltose binding protein. After diluting them, we set up a PCR with a variety of different colonies in order to try to PCR this gene about of the genome of E Coli.
Patrick
The previous day's R0040-E0430 and R0040-E0420 constructs grew colonies, but there was no fluorescence visible. I will leave this for another day just to see. As well, I selected five colonies from each plate of cpxP in pSB1A3. They have been put into an overnight culture for miniprepping tomorrow.
Dev
Today, I restriction digested the construction of E0040+B0015 that was constructed on Monday. This would help identify the identity of the construct. I expected to see bands in the 3000 and 800 range however, bands were found in the 4000-3000 range which could be uncut plasmids
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