Team:Calgary/1 August 2010

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Revision as of 13:46, 11 August 2010 by Emily Hicks (Talk | contribs)

Sunday August 1, 2010

Who wants to work on weekends? We do!!

Emily

Heck yes we do! This evening I made overnight cultures of my hypotically biobricked malE and malE31 in TOPO BLUNT Vector. I also set up an overnight digest of ......

Chris

Today, I went through PCR purifications of 100 µL of each of the CpxP Promoter PCR Products using a Qiagen kit with the same modified procedure as yesterday. This second purification was done to gain more purified product. Most of the first purification was used up in doing a restriction digest and attempting to construct the CpxP promoter into Ampicillin-kanamycin and ampicillin-chloramphenicol plasmids. I also did this construction today, cutting with the restriction enzymes XbaI and PstI.


Dev

Today I performed a plasmid switch of the CpxR promoter from an ampicillin resistant plasmid to an ampicillin-kanamycin resistant plasmid. Transformed the AK CpxR into competent cells and plated.

No notebook page exists for this date. Sorry!