Team:Calgary/1 August 2010

From 2010.igem.org

(Difference between revisions)
 
(3 intermediate revisions not shown)
Line 1: Line 1:
{{CalgaryNotebookTemplate|
{{CalgaryNotebookTemplate|
-
'''Sunday August 1, 2010'''
+
Sunday August 1, 2010|
Who wants to work on weekends? We do!!
Who wants to work on weekends? We do!!
 +
 +
<u>Emily</u>
 +
 +
Heck yes we do!  This evening I made overnight cultures of my hypotically biobricked malE and malE31 in TOPO BLUNT Vector.  I also set up an overnight digest of a construction of I0500-I13504 and I0500-I13507.  I want to construct these circuits as a control for our Arabinose Induction tests.
 +
<u>Chris</u>
<u>Chris</u>

Latest revision as of 04:54, 23 August 2010

Sunday August 1, 2010

Who wants to work on weekends? We do!!

Emily

Heck yes we do! This evening I made overnight cultures of my hypotically biobricked malE and malE31 in TOPO BLUNT Vector. I also set up an overnight digest of a construction of I0500-I13504 and I0500-I13507. I want to construct these circuits as a control for our Arabinose Induction tests.


Chris

Today, I went through PCR purifications of 100 µL of each of the CpxP Promoter PCR Products using a Qiagen kit with the same modified procedure as yesterday. This second purification was done to gain more purified product. Most of the first purification was used up in doing a restriction digest and attempting to construct the CpxP promoter into Ampicillin-kanamycin and ampicillin-chloramphenicol plasmids. I also did this construction today, cutting with the restriction enzymes XbaI and PstI.


Dev

Today I performed a plasmid switch of the CpxR promoter from an ampicillin resistant plasmid to an ampicillin-kanamycin resistant plasmid. Transformed the AK CpxR into competent cells and plated.