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Team:Calgary/10 June 2010
From 2010.igem.org
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| - | '''Thursday June 10, 2010''' | + | {{CalgaryNotebookTemplate|'''Thursday June 10, 2010''' |
Jeremy, Chris, Dev: Today, we spent much of the day researching new parts that we could use. Our TA mentioned the possible part of K145015 (GFP with an LVA tag) that we could use as a reporter, but the sequencing results from the Registry were inconsistent. We made overnight cultures of the parts E0032 and E0040 in pSB1K3 so they could be Miniprepped tomorrow. As well, we began the procedures for making competent cells by making overnight cultures with Top10 cells. Jeremy tried the transformation of I73005 (B-galactosidase gene) for the third time. We are unsure whether it will grow or not because the sequencing was listed as inconsistent on the Registry. | Jeremy, Chris, Dev: Today, we spent much of the day researching new parts that we could use. Our TA mentioned the possible part of K145015 (GFP with an LVA tag) that we could use as a reporter, but the sequencing results from the Registry were inconsistent. We made overnight cultures of the parts E0032 and E0040 in pSB1K3 so they could be Miniprepped tomorrow. As well, we began the procedures for making competent cells by making overnight cultures with Top10 cells. Jeremy tried the transformation of I73005 (B-galactosidase gene) for the third time. We are unsure whether it will grow or not because the sequencing was listed as inconsistent on the Registry. | ||
| - | Alex, Patrick, Raida: The previous day's R0040-E0430 construction was plated onto Amp agar today. It was probably not a good idea to plate it early in the day, since it may develop satellite colonies, but we will see tomorrow. | + | Alex, Patrick, Raida: The previous day's R0040-E0430 construction was plated onto Amp agar today. It was probably not a good idea to plate it early in the day, since it may develop satellite colonies, but we will see tomorrow.}} |
Revision as of 23:31, 21 June 2010
Thursday June 10, 2010
Jeremy, Chris, Dev: Today, we spent much of the day researching new parts that we could use. Our TA mentioned the possible part of K145015 (GFP with an LVA tag) that we could use as a reporter, but the sequencing results from the Registry were inconsistent. We made overnight cultures of the parts E0032 and E0040 in pSB1K3 so they could be Miniprepped tomorrow. As well, we began the procedures for making competent cells by making overnight cultures with Top10 cells. Jeremy tried the transformation of I73005 (B-galactosidase gene) for the third time. We are unsure whether it will grow or not because the sequencing was listed as inconsistent on the Registry.
Alex, Patrick, Raida: The previous day's R0040-E0430 construction was plated onto Amp agar today. It was probably not a good idea to plate it early in the day, since it may develop satellite colonies, but we will see tomorrow.
No notebook page exists for this date. Sorry!
