BIOTEC Dresden/Notepad/9 August 2010
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(New page: {{Biotec_Dresden/Header}} Gradient PCR using Phusion polymerase on plasmids: 11,27,28 <hr> {{Biotec_Dresden/month}} {{Biotec_Dresden/Bottom}}) |
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- | + | Check gradient PCR with Taq polymerase from 08.08.2010 on agarose gel on plasmids: | |
+ | 23e, 25a, 27e | ||
- | + | Gradient PCR using Fusion polymerase on plasmids: | |
- | + | 23e, 25a, 27e | |
+ | Assembly of the following parts were commenced with restriction digest and ligation: | ||
+ | |||
+ | 6e, 4a, 13e, 9b, 15e, 14f, 20f, 21f | ||
+ | |||
+ | |||
+ | The plasmid backbone used was pSB1C3. Two ligations were performed - one based on the standard protocol and the other samples were left for overnight ligation. The ligated parts were transformed by electroporation method and plated. | ||
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+ | <hr> | ||
+ | [[Category:BIOTEC Dresden/Notepad/August|August]] | ||
{{Biotec_Dresden/month}} | {{Biotec_Dresden/month}} | ||
{{Biotec_Dresden/Bottom}} | {{Biotec_Dresden/Bottom}} |
Latest revision as of 21:53, 27 October 2010
Check gradient PCR with Taq polymerase from 08.08.2010 on agarose gel on plasmids:
23e, 25a, 27e
Gradient PCR using Fusion polymerase on plasmids:
23e, 25a, 27e
Assembly of the following parts were commenced with restriction digest and ligation:
6e, 4a, 13e, 9b, 15e, 14f, 20f, 21f
The plasmid backbone used was pSB1C3. Two ligations were performed - one based on the standard protocol and the other samples were left for overnight ligation. The ligated parts were transformed by electroporation method and plated.
July |
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August |
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September |
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